Z-LEHD-FMK TFA

製品コードS7313 バッチS731305

印刷

化学情報

Synonyms

Caspase-9 Inhibitor

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₄H₄₄F₄N₆O₁₂

分子量

804.74

CAS No.

524746-03-0

Solubility (25°C)*

体外

DMSO

100 mg/mL (124.26 mM)

Ethanol

100 mg/mL (124.26 mM)

Water

50 mg/mL (62.13 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a specific, irreversible Caspase-9 inhibitor.
in vitro	Z-LEHD-FMK is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival^[2].
in vivo	Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HCT116 and SW480 cells
	濃度	20 μM
	反応時間	2 h
	実験の流れ	HCT116 and SW480 cells (as indicated) were pretreated with the inhibitors Z-IETD-FMK (caspase 8 inhibitor), Z-LEHD-FMK (caspase 9 inhibitor), Z-VDAVD-FMK (caspase 2 inhibitor), and Z-AEVD-FMK (caspase 10 inhibitor; 20 μm each) for 2 h, after which TRAIL (50 ng/ml) was added. After 4 h (or 16 h for caspase 3 activation) of TRAIL exposure, extracts were collected and analyzed by Western blotting for the expression of the different proteins.
動物実験	動物モデル	Male Wistar albino rats
	投薬量	0.8 μM/kg
	投与方法	i.v.

参考

https://pubmed.ncbi.nlm.nih.gov/11103780/
https://pubmed.ncbi.nlm.nih.gov/26850530/
https://pubmed.ncbi.nlm.nih.gov/15796358/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia [ Nat Commun, 2025, 16(1):918]	PubMed: 39843445
Caspase-9 activates β-catenin signaling to promote pulmonary fibrosis [ J Transl Med, 2025, 23(1):986]	PubMed: 40898190
Hnf4α integrates AIF and caspase 3/9 signaling to restrict single and coinfecting pathogens in teleosts [ PLoS Pathog, 2025, 21(9):e1013491]	PubMed: 40920830
Interferon alpha promotes caspase-8 dependent ultraviolet light-mediated keratinocyte apoptosis via interferon regulatory factor 1 [ Front Immunol, 2024, 15:1384606]	PubMed: 38660315
Receptor-interacting protein kinase 1 confers autophagic promotion of gasdermin E-mediated pyroptosis in aristolochic acid-induced acute kidney injury [ Ecotoxicol Environ Saf, 2024, 284:116944]	PubMed: 39208575
Pseudorabies virus gM and its homologous proteins in herpesviruses induce mitochondria-related apoptosis involved in viral pathogenicity [ PLoS Pathog, 2024, 20(4):e1012146]	PubMed: 38669242
Novel Diclofenac-NO Donor With High Affinity for Human Serum Albumin Induces Endoplasmic Reticulum Stress-mediated Cell Death in Human Pancreatic Cancer Cells [ Anticancer Res, 2024, 44(9):3793-3798]	PubMed: 39197907
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.]	PubMed: 37646198
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423]	PubMed: 37438979
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423]	PubMed: 37438979

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。