Z-YVAD-FMK

製品コードS8507 バッチS850702

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₃₁H₃₉FN₄O₉

分子量

630.66

CAS No.

210344-97-1

Solubility (25°C)*

体外

DMSO

100 mg/mL (158.56 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Z-YVAD-FMKは、抗炎症作用と抗腫瘍作用を持つ、細胞透過性の不可逆的なカスパーゼ-1特異的阻害剤です。
in vitro	Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. This compound remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.
in vivo	Z-YVAD-FMK is a cell-permeable caspase-6 inhibitor.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	BV2 cells
	濃度	5 μM
	反応時間	1 h
	実験の流れ	BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.
動物実験	動物モデル	C57BL/6J mice
	投薬量	50 μM
	投与方法	i.v.

参考

https://pubmed.ncbi.nlm.nih.gov/31374144/
https://pubmed.ncbi.nlm.nih.gov/29545090/
https://pubmed.ncbi.nlm.nih.gov/32999158/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages [ Cells, May 12 2022, 1630]	PubMed: 35626667
Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation [ Cell Reports Medicine, July 18 2023, 101098]	PubMed: 37390829
Progesterone Enhances Sensitivity of Ovarian Cancer Cells to SN38 Through Inhibition of Topoisomerase I and Inducing Ferroptosis [ Cancer Reports, April 2025, e70202]	PubMed: 40270435
Liver Sinusoidal Endothelial Cells Promote Metabolic Dysfunction-associated Steatohepatitis Progression via Interleukin-1R1-mediated Chemokine Production Induced by Macrophage-derived Interleukin-1β [ Cellular and Molecular Gastroenterology and Hepatology, 2025, 101698]	PubMed: 41344438
PRRSV-2 nsp2 Ignites NLRP3 inflammasome through IKKβ-dependent dispersed trans-Golgi network translocation [ PLoS Pathog, 2025, 21(1):e1012915]	PubMed: 39869629
Progesterone Enhances Sensitivity of Ovarian Cancer Cells to SN38 Through Inhibition of Topoisomerase I and Inducing Ferroptosis [ Cancer Rep (Hoboken), 2025, 8(4):e70202]	PubMed: 40270435
Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats [ Transl Psychiatry, 2024, 14(1):166]	PubMed: 38538614
3-Acetyldeoxynivalenol induces pyroptosis in leydig cells via METTL3-mediated N6-methyladenosine modification of NLRP3 [ Ecotoxicol Environ Saf, 2024, 290:117549]	PubMed: 39701865
Role of the ROS/NLRP3/caspase-1 pathway in NiSO4-induced cellular pyroptosis and apoptosis in H9c2 cells [ Toxicology, 2024, 509:153989]	PubMed: 39515573
Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation [ Cell Reports Medicine, 2023, 101098]	PubMed: nan

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。