Z-YVAD-FMK

製品コードS8507 バッチS850702

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₃₁H₃₉FN₄O₉
	分子量	630.66	CAS No.	210344-97-1
Solubility (25°C)*	体外	DMSO	100 mg/mL (158.56 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.
in vitro	Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. Z-YVAD-FMK remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.^[1]
in vivo	Z-YVAD-FMK is a cell-permeable caspase-6 inhibitor.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	BV2 cells
	濃度	5 μM
	反応時間	1 h
	実験の流れ	BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.
動物実験	動物モデル	C57BL/6J mice
	投薬量	50 μM
	投与方法	i.v.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats [ Transl Psychiatry, 2024, 14(1):166]	PubMed: 38538614
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.]	PubMed: 37646198
Mannose-doped metal-organic frameworks induce tumor cell pyroptosis via the PERK pathway [ J Nanobiotechnology, 2023, 21(1):426]	PubMed: 37968665
Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages [ Cells, 2022, 11(10)1630]	PubMed: 35626667
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis [ Mediators Inflamm, 2022, 2022:8007078]	PubMed: 35497095
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis [ Mediators Inflamm, 2022, 2022:8007078]	PubMed: 35497095
Gain-of-function hot spot mutant p53R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma [ Transl Oncol, 2021, 14(1):100982]	PubMed: 33395748
Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12/15-LOX signaling [ Biomed Pharmacother, 2020, 127:110151]	PubMed: 32559840
Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury [ Biol Pharm Bull, 2020, 43(10):1481-1489]	PubMed: 32999158

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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