Erastin

製品コードS7242

Erastin化学構造

分子量(MW):547.04

Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.

サイズ 価格(税別)  
JPY 24402.00
JPY 127820.00

カスタマーフィードバック(8)

  • Indicated HCC cells were treated with sorafenib (5 μM) and erastin (10 μM) with or without cell death inhibitors (ferrostatin-1, 1 μM; liprostatin-1, 100 nM; ZVAD-FMK, 10 μM; necrosulfonamide, 0.5 μM) for 24 hours, and cell viability was assayed (n=3, *P < 0.05 versus sorafenib or erastin treatment group).

    Hepatology, 2016, 64(2):488-500.. Erastin purchased from Selleck.

    Western blot analysis indicated protein expression in PDAC cells following treatment with erastin (2.5-40 μmol/L) for 24 hours (n=3, * P < 0.05 vs. untreated group)

    Cancer Res, 2017, 77(8):2064-2077. Erastin purchased from Selleck.

  • Erastin induces HSF1-dependent HSPB1 expression in human cancer cells. Indicated human cancer cells were treated with erastin (HeLa, 0.5 μM; U2OS, 5 μM; LNCaP, 5 μM) for 24 h and the protein expressions of indicated HSPs were assayed by western blot.

    Oncogene, 2015, 10.1038/onc.2015.32. Erastin purchased from Selleck.

    HeLa cells were treated with erastin (0.5 μM) for 24 h with or without Gö 6893 (0.5 μM) and calphostin C (0.1 μM), and HSPB1 phosphorylation at Ser 15 was assayed using western blot.

    Oncogene, 2015, 10.1038/onc.2015.32. Erastin purchased from Selleck.

  • F. Dramatically augmentation of the EF24 cytotoxicity to gastric cancer cells by GSH depletion. G. EF24 and erastin in combination dramatically activated ER-stress pathway.

    Oncotarget, 2016, 7(14):18050-64.. Erastin purchased from Selleck.

    Erastin exerts cytotoxic, but not cytostatic, effects to cultured colorectal cancer cells. Colorectal cancer cells (HT-29, DLD-1 and Caco-2 lines) or NCM460 colon epithelial cells were treated with vehicle control (0.1% DMSO, “Ctrl”) or indicated concentrations of erastin for applied time, cell survival was tested by MTT assay (A and E) and colony formation assay (C); The percentage of trypan blue positive (“dead” cells) was recorded (B); Cell proliferation was tested by BrdU incorporation assay (D and F). For each assay, n = 5. The data presented were mean ± SD. Experiments were repeated three times with similar results obtained. * p < 0.05 vs. group of “Ctrl”.

    PLoS One, 2016, 11(5):e0154605.. Erastin purchased from Selleck.

  • Baicalein suppresses erastin-induced GPX4 degradation. (A) PANC1 and BxPc3 cells were treated with erastin (20 μM) in the absence or presence of baicalein (10 μM) for 24 h. The indicated protein levels were assayed using western blot. (B) In parallel, the relative intensity of the western blot band of GPX4 was quantified using ImageJ densitometry software (n = 3, *, p < 0.05).

    Biochem Biophys Res Commun, 2016, 473(4):775-80.. Erastin purchased from Selleck.

    FANCD2 suppresses erastin-induced ferroptosis in BMSCs. (A) FANCD2+/+ and FANCD2 −/− BMSCs were treated with erastin (0.625–2.5 μM) for 24 h and cell viability was assayed (n = 3, *P < 0.05). (B) Interference contrast images of FANCD2+/+ and FANCD2 −/− BMSCs with or without erastin (1.25 μM) treatment for 24 h (C) FANCD2+/+ and FANCD2 −/− BMSCs were treated with erastin (1.25 μM) in the absence or presence of ferroptosis inhibitor (ferrostatin-1, 500 nM; liprostatin-1, 200 nM; β-mercaptoethanol, 50 μM; N-acetylcysteine, 100 mM) or autophagy inhibitor (chloroquine, 10 μM; 3-methyladenine, 5 mM) for 24 h. Cell viability was assayed (n = 3, *P < 0.05 versus erastin treatment group). (D) Western blot analysis of LC3-I and LC3-II expression in FANCD2+/+ and FANCD2 −/− BMSCs following erastin (1.25 μM) treatment for 24 h. (E) In parallel, the LC3-II/I ratio quantified by densitometry analysis of bands.

    Biochem Biophys Res Commun, 2016, 480(3):443-449.. Erastin purchased from Selleck.

製品安全説明書

Ferroptosis阻害剤の選択性比較

生物活性

製品説明 Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS.
ターゲット
Ferroptosis [1]
体外試験

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2] Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3] In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect of cisplatin in WT EGFR cells. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CCF-STTG1 cells NIrWO5NHfW6ldHnvckBie3OjeR?= MVjJcohq[mm2aX;uJI9nKFildDDpckBpfW2jbjDDR2YuW1SWR{GgZ4VtdHNiYYPz[ZN{\WRiYYOg[4x2fGGvYYTlJJJmdGWjc3WgZYZ1\XJiMjDodpMh[nliZnz1c5JwdWW2comsJGlEPTB;MD6yJO69VS5? NVHaSopGOjZ{M{GxOVY>
human HeLa cells NETOcHBHfW6ldHnvckBie3OjeR?= MojFTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSHXMZUBk\WyuczygSWM2OD1yLk[g{txONg>? NVm2WHFpOTd3Nki3OFg>
human BJ cells MWPGeY5kfGmxbjDhd5NigQ>? MlXFTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQlqgZ4VtdHNiZYjwdoV{e2mwZzDUSXJVNCCOVDygV3Qh[W6mIGLBV{BIOTKYIH31eIFvfCCpZX7ld{Bk\WyuczDpckBxemW|ZX7j[UBw\iCSRD25PFA2QSCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44hdWW2aH;kMEBKSzVyPUCuPUDPxE1w NFjIW5kyPzV4OEe0PC=>
human HT1080 cells NH63eZpHfW6ldHnvckBie3OjeR?= M1nVb2lv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFjUNVA5OCClZXzsd{BqdiCycnXz[Y5k\SCxZjDQSE06QDB3OTDifUB1enmyYX6gZox2\SCneHPseZNqd25ibXX0bI9lNCCLQ{WwQVEh|ryPLh?= MYSxO|U3QDd2OB?=
human SVR cells NH;teWRHfW6ldHnvckBie3OjeR?= NF7o[4xKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDTWnIh[2WubIOsJGVEPTB;Mj61JO69VS5? MkDWNVc2Pjh5NEi=
human MES-SA cells NFHtb5hHfW6ldHnvckBie3OjeR?= MlTRTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iTVXTMXNCKGOnbHzzMEBGSzVyPUOg{txONg>? NVjT[VRoOTd3Nki3OFg>
human SKUT cells MmPRSpVv[3Srb36gZZN{[Xl? NEL6OYxKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDTT3VVKGOnbHzzMEBGSzVyPUSg{txONg>? M1SzclE4PTZ6N{S4
human Calu1 cells Ml3uSpVv[3Srb36gZZN{[Xl? M2nJXWlvcGmkaYTpc44hd2ZiaIXtZY4hS2GudUGgZ4VtdHNiZYjwdoV{e2mwZzDLVmFUKHerdHigZYN1cX[jdHnu[{BufXSjdHnvcpMh[nlidIL5dIFvKGKudXWg[ZhkdHW|aX;uJIF{e2G7LDDJR|UxRTRizszNMi=> MmDqNVc2Pjh5NEi=
human LNCaP cells M3:wc2Z2dmO2aX;uJIF{e2G7 MUPJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCOTlPhVEBk\WyuczygSWM2OD14IN88UU4> Mnu0NVc2Pjh5NEi=
human U2OS cells NFOzVmRHfW6ldHnvckBie3OjeR?= MnTFTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iVULPV{Bk\WyuczygSWM2OD14IN88UU4> NEXoNmsyPzV4OEe0PC=>
human TC32 cells MYPGeY5kfGmxbjDhd5NigQ>? MWfJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDoeY1idiCWQ{OyJINmdGy| NHWxdGoyPzV4OEe0PC=>
human SK-N-MC cells MmP4SpVv[3Srb36gZZN{[Xl? MnnzTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iU1utUk1OSyClZXzsd{whTUN3ME2xNEDPxE1w MYixO|U3QDd2OB?=
human U937 cells MVHGeY5kfGmxbjDhd5NigQ>? NHnSZXhKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDVPVM4KGOnbHzzMEBGSzVyPUGwJO69VS5? M1LyflE4PTZ6N{S4
human TC71 cells MmfCSpVv[3Srb36gZZN{[Xl? NHL0O5RKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDUR|cyKGOnbHzzMEBGSzVyPUGwJO69VS5? Mkn6NVc2Pjh5NEi=
human BJ cells MVXGeY5kfGmxbjDhd5NigQ>? MYrJcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCrbjDUSXJVKGW6cILld5NqdmdiaIXtZY4hSkpiY3XscJMtKEWFNUC9NVAh|ryPLh?= MYixO|U3QDd2OB?=
human EWS502 cells M1;qS2Z2dmO2aX;uJIF{e2G7 M374V2lv\HWldHnvckBw\iClZXzsJIRm[XSqIHnuJIh2dWGwIFXXV|UxOiClZXzsd{whTUN3ME2xNEDPxE1w NUPNZZA4OTd3Nki3OFg>
human Hs51.T cells NH7sV|JHfW6ldHnvckBie3OjeR?= Mmf6TY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSIO1NU5VKGOnbHzzMEBGSzVyPUGyJO69VS5? NXnqUW5lOTd3Nki3OFg>
human Hs925.T cells NX6ySJd6TnWwY4Tpc44h[XO|YYm= MojYTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iSIO5NlUvXCClZXzsd{whTUN3ME2xO{DPxE1w M2r1c|E4PTZ6N{S4
human HOS cells NFvjZ|RHfW6ldHnvckBie3OjeR?= NVLxfmFpUW6mdXP0bY9vKG:oIHPlcIwh\GWjdHigbY4hcHWvYX6gTG9UKGOnbHzzJEwhTUN3ME2xO{DPxE1w Mke2NVc2Pjh5NEi=
human MX2 cells M4DkN2Z2dmO2aX;uJIF{e2G7 NIrXcGZKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDNXFIh[2WubIOsJGVEPTB;MUig{txONg>? NGTYRYIyPzV4OEe0PC=>
human A673 cells NWTGU5BCTnWwY4Tpc44h[XO|YYm= NHr3d5ZKdmS3Y4Tpc44hd2ZiY3XscEBl\WG2aDDpckBpfW2jbjDBOlc{KGOnbHzzMEBGSzVyPUOwJO69VS5? MXuxO|U3QDd2OB?=
human BJ cells NGr4e3VHfW6ldHnvckBie3OjeR?= MlTwTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQlqgZ4VtdHNiZYjwdoV{e2mwZzDUSXJVNCCOVDygV3Qh[W6mIGLBV{BIOTKYIH31eIFvfCCpZX7ld{BqdiCycnXz[Y5k\SCxZjDVNFEzPiCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44hdWW2aH;kMEBKSzVyPUOxMlIh|ryPLh?= NWf3c5d4OTd3Nki3OFg>
human BJ cells MnTzSpVv[3Srb36gZZN{[Xl? MkK2PUDPxE1? MmqzTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gbJVu[W5iQlqgZ4VtdHNiZYjwdoV{e2mwZzDUSXJVNCCOVDygV3Qh[W6mIGLBV{BIOTKYIH31eIFvfCCpZX7ld{Bk\WyuczDheEA6KHWPLh?= MlHWNVc2Pjh5NEi=
human BJ cells NYj3SI03TnWwY4Tpc44h[XO|YYm= M3LBZVQvPiEQvF2= MU\JcoNz\WG|ZTDpckBqdnS{YXPlcIx2dGG{IH;4bYRifGm4ZTDzdIVkcWW|IHnuJIh2dWGwIFLKJINmdGy|IHX4dJJme3OrbnegWGVTXCxiTGSsJHNVKGGwZDDSRXMhTzF{VjDteZRidnRiZ3Xu[ZMh[2WubIOgZZQhPC54IIXN NF;1RVgyPzV4OEe0PC=>
human BJeLR cells MmryR5l1d3SxeHnjxsBie3OjeR?= M1XnT|ExKM7:TR?= NEDITXcyOiCq MlLuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmpmVFJiY3XscJMh\XiycnXzd4lv\yCUQWOgS|EzXiCvdYThcpQh[XRiMUCgeW0h[XRiMUKgbJJ{KGK7IITyfZBidiCkbIXlJJN1[WmwaX7n MlPONlI5OzJ|MkG=
human BJeH cells MXPGeY5kfGmxbjDhd5NigQ>? M1roTVYhcA>? NFLrZ45KdmS3Y4Tpc44hd2ZicnXhZ5RqfmVib4j5[4VvKHOyZXPp[ZMheHKxZIXjeIlwdiCrbjDoeY1idiCESnXIJINmdGy|IHX4dJJme3Orbnege4lt\CC2eYDlJHJCWyCjZoTldkA3KGi{czDifUBFS0ZvYnHz[YQh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz NH[zU5YzOjh|MkOyNS=>

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

お薦めの試験操作(参考用のみ)

細胞試験: [1]
+ 展開
  • 細胞株: BJ-TERT/LT/ST/RASV12 cells
  • 濃度: 5 or 10 μg/mL
  • 反応時間: 6-11 hours
  • 実験の流れ: BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 19 mg/mL (34.73 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+corn oil
混合させたのち直ちに使用することを推奨します。
2.5mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 547.04
化学式

C30H31ClN4O4

CAS No. 571203-78-6
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須
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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID