Epigenetics Compound Library

目录号 L1900


サイズ 価格(税別)  
100uL/well (10mM solution) JPY 814396.00
2x100uL/well (10mM solution) JPY 1428596.00


Epigenetics Compound Library内容


  • Differential effects of HDAC inhibitors on histone and tubulin acetylation. Immunofluorescence analysis of histone H3 (K9ac/K14ac) and tubulin acetylation in HeLa cells treated for 4 h with vehicle, SAHA (10 μM), tacedinaline (50 μM), PCI-24781 (20 μM. (a) Mapping of histone acetylation in K562 cells treated with HDAC inhibitors by LC-MS/MS. Cells were treated with TSA (10 μM), SAHA (5 μM), PCI-24781 (2 μM), tacedinaline (50 μM) for 6 h. Histones were extracted from cells and acetylated peptides were quantified after isobaric tagging.

    Nat Biotechnol 2011 29, 255-265. Vorinostat (SAHA, MK0683) purchased from Selleck

    Targeting PI3K, a common downstream effector of RTKs, with a selective inhibitor (GDC0941) sensitizes SOX10 knockdown cells to vemurafenib. shRNAs targeting SOX10 were introduced into A375 cells by lentiviral transduction. pLKO.1 empty vector served as a control vector (Ctrl). Cells were seeded in 6-well plates at the same density in the presence or absence of drug(s) at the indicated concentration. Cells were cultured for 2 weeks in the absence of vemurafenib or 4 weeks in the presence of vemurafenib before fixing and staining.

    Nature 2014 508(7494), 118-22. Barasertib (AZD1152-HQPA) purchased from Selleck

  • Activity of vorinostat in Jurkat and Peer T-ALL cell lines in an MTT cell viability assay. Cells were treated with increasing drug concentrations for 72 h. The data are plotted as the mean % of DMSO-treated control cells against the corresponding drug concentration. The error bars are the standard error. For each drug and cell line, 3-4 independent experiments were performed with 6 replicates at each drug concentration.

    Nature 2011 471, 235-9. Vorinostat (SAHA, MK0683) purchased from Selleck

    (G) Nocodazole-arrested HeLa cells were treated with VX-680 and MG132 and stained for CENP-E (Green), pT422 (Red) and DNA (Blue). (H) pT422 fluorescence intensity was normalized to the total CENP-E fluorescence. Plots show the mean of > 15 cells per condition from two independent experiments.

    Cell 2010 142, 444–455. Tozasertib (VX-680, MK-0457) purchased from Selleck

  • Immunoblot analysis of PARylation after treatment with various PARP inhibitors.The asterisk indicates a nonspecific band.

    Nat Methods 2013 10(10), 981-4. Iniparib (BSI-201) purchased from Selleck

    Inhibition of Aurka kinase activity by MLN8237 impairs expression of pluripotency genes in CCE cells as measured by qRT-PCR. All values shown are mean ?SEM for n=3. The level of phosphorylated H3(S10) (p-H3(S10)), an Aurka phosphorylation target site, is decreased in MLN8237-treated samples.

    Cell Stem Cell 2012 11, 179-94. Alisertib (MLN8237) purchased from Selleck

  • Effects of CP-690550 on NKTCL cell lines. NK-S1, KHYG-1 cells were treated with CP-690550 for 48 hours, and the effect on STAT5 phosphorylation was evaluated by Western blotting.

    Cancer Discov 2012 2(7), 591-7. Tofacitinib (CP-690550,Tasocitinib) purchased from Selleck

    IL-6- supported INA-6 cells were treated with the JAK inhibitors ruxolitinib (Rux; 10 nM) or CYT387 (CYT; 50 nM) for 1 hour and assessed for inhibition of STAT3 phosphorylation by immunoblotting.

    J Clin Invest 2014 10.1172/JCI69094. Momelotinib (CYT387) purchased from Selleck

  • Primary MKPs were treated with the Aurora B inhibitor AZD-1152, and then stimulated with 20 ng/ml TPO for 5 d. Cell morphology was analyzed by Giemsa staining (Bar, 20 祄; red arrows denote mature MKs; n = 6).

    J Exp Med 2014 10.1084/jem.20141123. Barasertib (AZD1152-HQPA) purchased from Selleck

    Control and MEC17 KD macrophages (RAW264.7) were treated with TBSA or DMSO for 12 hours followed by LPS treatment for indicated time. p38 phosphorylation were determined by immuno-blotting.

    Nat Commun 2014 5, 3479. Tubastatin A HCl purchased from Selleck


• 生物活性を持っている181種類の小分子調節剤(阻害剤と活性剤)の集めはepigenetics研究と関連実験に使用して、多種な構造と異なる機能を含めます。
• 可利用道具として、ファーマコジェノミクスや他の生物学応用で化学ジェノミクスと後成学のターゲットを識別します。
• このライブラリーは、HDACs、SIRTs、Lysine methylation enzyme、HATs、DnmtsとSIRTs活性剤を含む後成的な酵素の抑制剤を含みます。
• 構造多様で、効果が目立って、細胞浸透可。
• 十分に詳しい構造説明、,IC50値とお客様からフィードバックした資料があります。
• NMRとHPLC技術で製品の高純度を保証します。 


調合: 181種類の小分子調節剤(阻害剤と活性剤)は予めDMSO溶液に溶解する
96穴板: 96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode
安定性: in DMSO
出荷方式: ブルーアイス物流
包装: 不活性ガス(Inert gas)

Epigenetics Compound Library製品説明

HTS パートナ