Bafilomycin A1 (Baf-A1)

バフィロマイシン A1 (Bafilomycin A1(Baf-A1)) は液胞 H+-ATPase 阻害剤で、IC50 は 0.44 nM です。 バフィロマイシン A1 は、オートファジー (autophagy) を阻害する一方で、アポトーシス (apoptosis) を誘導することがわかっています。

Bafilomycin A1 (Baf-A1)化学構造

CAS No. 88899-55-2

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Bafilomycin A1 (Baf-A1)と併用されることが多い化合物

3-MA (3-Methyladenine)


Bafilomycin A1 and 3-Methyladenine (3-MA) combination plus irradiation leads to more pronounced and prolonged DNA damage in glioma and breast cancer cells.


Tameire F, et al. Semin Cancer Biol. 2015 Aug;33:3-15.

TMZ(Temozolomide)


Bafilomycin A1 and Temozolomide enhance cytotoxicity and induce autophagic death signaling in glioblastoma multiforme treatment.


Jawhari S, et al. Cell Death Dis. 2016 Oct 27;7(10):e2434.

Chloroquine


Bafilomycin A1 (Baf-A1) and Chloroquine combination inhibit autophagy in acute myeloid leukemia (AML) by blocking autophagosome formation.


Hwang DY, et al. J Exp Clin Cancer Res. 2020 May 11;39(1):85.

Hydroxychloroquine (HCQ) Sulfate


Bafilomycin A1 (Baf-A1) and Hydroxychloroquine (HCQ) Sulfate combination inhibit autophagy by blocking autophagosome formation in the treatment of acute myeloid leukemia (AML).


Hwang DY, et al. J Exp Clin Cancer Res. 2020 May 11;39(1):85.

Vorinostat (SAHA)


Bafilomycin A1 and Vorinostat (SAHA) might give a therapeutic advantage for the treatment of malignancies and missense mutations.


Foggetti G, et al. Biosci Rep. 2019 Feb 19;39(2):BSR20181345.

Bafilomycin A1 (Baf-A1)関連製品

シグナル伝達経路

Proton Pump阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
mouse RAW264.7 cells Apoptosis assay 100 nM 16 h Induction of apoptosis in mouse RAW264.7 cells assessed as late apoptotic cells at 100 nM after 16 hrs using annexin V-propidium iodide staining by flow cytometry 19307359
human H4 cells Function assay 0.4 μM 24 h Induction of light chain 3-GFP level in human H4 cells at 0.4 uM after 24 hrs by high throughput fluorescence microscopy relative to control 18024584
RAW 264.7 cells Bactericidal activity assay 100 nM 16 h Bactericidal activity against Salmonella enterica Typhimurium 14028 infected in RAW 264.7 cells assessed as decrease in bacterial growth yield at 100 nM after 16 hrs postinfection by flow cytometry in presence of 10 ug/ml of intracellular replication inhi 19307359
RAW 264.7 cells Antimicrobial activity assay 100 nM 30 mins Antimicrobial activity against Salmonella enterica Typhimurium 14028 infected in RAW 264.7 cells assessed as inhibition of bacterial replication at 100 nM treated 30 mins before infection measured after 2 to 16 hrs postinfection by flow cytometry 19307359
human HeLa cells Function assay 400 nM Induction of autophagy in human HeLa cells expressing EGFP-LC3 assessed as increase in LC3-2 level at 400 nM 18391949
RAW 264.7 cells Function assay 100 nM Antimicrobial activity against Salmonella enterica Typhimurium 14028 infected in RAW 264.7 cells assessed as increased nitric oxide production in infected cells at 100 nM 19307359
RAW 264.7 cells Antimicrobial activity assay 100 nM Antimicrobial activity against Salmonella enterica Typhimurium 14028 infected in RAW 264.7 cells assessed as increased nitric oxide production in infected cells at 100 nM 19307359
human MCF7 cells Function assay 4 h Inhibition of rapamycin-induced autophagy in human MCF7 cells expressing EGFP-LC3 assessed as decrease in EGFP levels at 100 nM after 4 hrs by Western blotting relative to control 20028134
human Huh7.5.1 cells Antiviral activity 3 h Antiviral activity against HCV genotype 2a JFH-1 in human Huh7.5.1 cells assessed as reduction of viral entry up to 3 hrs by luciferase assay 26396683
rat 3Y1 cells Function assay Induction of morphological changes in rat 3Y1 cells assessed as elongation of cells 29701963
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生物活性

製品説明 バフィロマイシン A1 (Bafilomycin A1(Baf-A1)) は液胞 H+-ATPase 阻害剤で、IC50 は 0.44 nM です。 バフィロマイシン A1 は、オートファジー (autophagy) を阻害する一方で、アポトーシス (apoptosis) を誘導することがわかっています。
Targets
H+-ATPase [1]
(Cell-free assay)
0.44 nM
In Vitro
In vitro Bafilomycin A1 is a toxic macrolide antibiotic derived from Streptomyces griseus. Bafilomycin A1 inhibits the short circuit current induced by the outer mantle epithelium (OME). The IC50 and maximum inhibition dose of Bafilomycin A1 are 0.17 μM and 0.5 μM, respectively. [2] In addition, Bilomycin A1 inhibits the acid influx with an IC50 value of 0.4 nM. Bafilomycin A1 inhibits the acidification dose-dependently resulting in a lower quenching, and thus a higher fluorescence. [3] Bafilomycin A1 prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. [4] Bafilomycin A1 also affects the transport of endocytosed material from early to late endocytic compartments. Bafilomycin not only dissipates the low endosomal pH but also blocks transport from early to late endosomes in HeLa cells. [5] Bafilomycin A1 at doses of 0.1-1 μM completely inhibits the acidification of lysosomes revealed by the incubation with acridine orange in BNL CL.2 and A431 cells. [6] When Bafilomycin A1 is added to Hanks' balanced salt solution, endogenous protein degradation is strongly inhibited and numerous autophagosomes accumulated in H-4-II-E cells. Bafilomycin A1 also prevents the appearance of endocytosed HRP in autophagic vacuoles. [7]
Kinase Assay ATPase enzyme activity assays
The ATPase enzyme assay medium contains 6 mM MgSO4, 50 mM HEPES (pH 7.4), 200 mM Na2SO3 (V-ATPase activator), 0.5 mM sodium ortho-vanadate (P-ATPase inhibitor), 0.5 mM sodium azide (F-ATPase inhibitor) and 3 mM Na2ATP. This medium (1.0 mL), with or without the addition of the V-type ATPase inhibitor bafilomycin A1, is incubated with the filtered homogenate (0.1 mL) for 60 minutes at 23–25 °C. The reaction is stopped by the addition of 1 mL of TCA 3%. Spectrometric blanks are prepared as for the enzyme assay with the exception that the tissue sample is added after the acid. Phosphate analysis is accomplished by adding 2 mL of 1-butanol and 0.2 mL molybdate solution (5 g ammonium molybdate, 22 mL H2SO4 to 100 mL). After vortexing for 15 seconds the solution is neutralised with 0.5 mL citrate solution (100 g/500 mL, pH 7.0) and again vortexed for 15 seconds. The solution is then centrifuged (2000 × g; 3 minutes) to separate the butanol phase and the absorbance of this phase is read at 400 nm. Standards of orthophosphate are prepared (0.1 μM–2.0 μM) and treated in the same way as the enzyme activity assays. Enzyme activity is expressed in μmol of orthophosphate liberated per hour and per milligram of protein. V-ATPase activity is considered to be the difference between the total ATPase activity measured in the presence of Na2SO3, sodium orthovanadate and sodium azide and the ATPase activity measured in the presence of these reagents and of the specific V-ATPases inhibitor Bafilomycin A1.
細胞実験 細胞株 HeLa cells
濃度 ~20 nM
反応時間 20 hours
実験の流れ

H. pylori bacteria extract is treated with inhibitors, before addition to HeLa cells, as follows: DCCD 10 mM for 1 hour at 30 ºC; NBD-CI 100 μM for 1 hour at 30 ºC and the reaction is blocked with glycine 10 mM final concentration; NEM 275 μM for 1 hour at 30 ºC and the reaction is blocked by addition of β-mercaptoethanol 275 mM; Mg-ATP 14 μM for 1 hour at 0 ºC; 100 μM KNO3 and 14 μM Mg-ATP for 1 hour at 30 μM; NaCO3 100 μM, pH 11 for 1 hour at 0 ºC. The bacterial extract is then added to cell with a 40-fold dilution at a final concentration of 0.65 mg/mL. Controls are HeLa cells incubated with untreated bacterial extracts and cells treated with inhibitor Bafilomycin A1 under the same conditions as bacterial extracts, at the same concentrations or after a 40-fold dilution. The vacuotating activity of the bacterial extracts is assayed.

実験結果図 Methods Biomarkers 結果図 PMID
Western blot Ret / EGFR / p75 NGFR 21559479
Growth inhibition assay Cell viability 20534000
Immunofluorescence AIF LC3 25512644
ELISA TNF-alpha 26240140
In Vivo
In Vivo Bafilomycin A1 (1 μM and 0.1 μM) completely inhibits the resorptive activity of cultured osteoclasts. [8] Bafilomycin A1 dose-dependently inhibits the rate of Na+ uptake in young tilapia with a Ki of 0.16 μM. [9]
動物実験 動物モデル Young (approximately 9 days old) Mozambique tilapia, Oreochromis mossambicus
投与量 10 μM
投与経路 --

化学情報

分子量 622.83 化学式

C35H58O9

CAS No. 88899-55-2 SDF Download Bafilomycin A1 (Baf-A1) SDFをダウンロードする
Smiles CC1CC(=CC=CC(C(OC(=O)C(=CC(=CC(C1O)C)C)OC)C(C)C(C(C)C2(CC(C(C(O2)C(C)C)C)O)O)O)OC)C
保管

In vitro
Batch:

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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よくある質問(FAQ)

質問1:
How to dissolve it?

回答
S1413 Bafilomycin A1(Baf-A1) is soluble in DMSO at 6 mg/ml. Please do not use alcohols as solvent, because this compound will degrade in alcohols.

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