Bafilomycin A1(Baf-A1)

製品コードS1413

Bafilomycin A1(Baf-A1)化学構造

分子量(MW):622.83

Bafilomycin A1(Baf-A1)は、空胞の H+-ATPase阻害剤で、 IC50 が 0.44 nMです。

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JPY 34362.00 あり
JPY 100762.00 あり

カスタマーフィードバック(3)

  • Expressions of LC3-I and LC3-II in protein were detected by Western blotting. Bafilomycin A1 significantly inhibited the effect of PNU282987 on LC3-II/I ratio in LPS-stimulated BV2 microglia (n = 6 per group).

    Front Immunol, 2017, 8:553. Bafilomycin A1(Baf-A1) purchased from Selleck.

    High glucose levels inhibit autophagic flux in cultured neonatal rat cardiomyocytes. c, Representative immunofluorescent NRCs expressing mRFP-GFP-LC3. *P < 0.05 vs. the autophagosomes in the corresponding group without bafilomycin A1 treatment; # P < 0.05 vs. the autolysosomes in the corresponding group without bafilomycin A1 treatment. Experiments were repeated four times. G5.5, glucose 5.5 mmol/L; G25, glucose 25 mmol/L; Ang II, angiotensin II; Baf, bafilomycin A1

    Cardiovasc Diabetol, 2016, 15(1):136.. Bafilomycin A1(Baf-A1) purchased from Selleck.

  • (B) U87-MG stable cell lines were serum starved for 24 h, then treated with EGF (50 ng/mL) along with DMSO (10 μM) or bafilomycin A1 (Baf-A1) (1 μM) or MG132 (10 μM) for 6 h. The lysates were subjected to western blot with the indicated antibodies. Densitometry quantification of T-EGFR/Tubulin protein levels was shown

    FEBS Lett, 2016, 590(9):1345-53. Bafilomycin A1(Baf-A1) purchased from Selleck.

質量管理及び製品安全説明書

Proton Pump阻害剤の選択性比較

生物活性

製品説明 Bafilomycin A1(Baf-A1)は、空胞の H+-ATPase阻害剤で、 IC50 が 0.44 nMです。
ターゲット
H+-ATPase [1]
(Cell-free assay)
0.44 nM
体外試験

Bafilomycin A1 is a toxic macrolide antibiotic derived from Streptomyces griseus. Bafilomycin A1 inhibits the short circuit current induced by the outer mantle epithelium (OME). The IC50 and maximum inhibition dose of Bafilomycin A1 are 0.17 μM and 0.5 μM, respectively. [2] In addition, Bilomycin A1 inhibits the acid influx with an IC50 value of 0.4 nM. Bafilomycin A1 inhibits the acidification dose-dependently resulting in a lower quenching, and thus a higher fluorescence. [3] Bafilomycin A1 prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. [4] Bafilomycin A1 also affects the transport of endocytosed material from early to late endocytic compartments. Bafilomycin not only dissipates the low endosomal pH but also blocks transport from early to late endosomes in HeLa cells. [5] Bafilomycin A1 at doses of 0.1-1 μM completely inhibits the acidification of lysosomes revealed by the incubation with acridine orange in BNL CL.2 and A431 cells. [6] When Bafilomycin A1 is added to Hanks' balanced salt solution, endogenous protein degradation is strongly inhibited and numerous autophagosomes accumulated in H-4-II-E cells. Bafilomycin A1 also prevents the appearance of endocytosed HRP in autophagic vacuoles. [7]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells NXL1U4RiTnWwY4Tpc44h[XO|YYm= NWn6e3dZPCCq MojUTY5pcWKrdHnvckBw\iC{YYDhcZlkcW5vaX7keYNm\CCjdYTvdIhi\3liaX6gbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO|aX7nJGVITlBvTFOzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIFXHSnAhdGW4ZXzzJIF1KDFyMDDuUUBi\nSncjC0JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIILlcIF1cX[nIITvJINwdnS{b3y= MUCyNFAzQDF|NB?=
human HeLa cells NUjhS2p3TnWwY4Tpc44h[XO|YYm= MnrrOFAxKG6P NHPQZYtKdmS3Y4Tpc44hd2ZiYYX0c5Bp[We7IHnuJIh2dWGwIFjlUIEh[2WubIOg[ZhxemW|c3nu[{BGT0[SLVzDN{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDMR|MuOiCuZY\lcEBifCB2MECgcm0> Ml;ZNVg{QTF7NEm=
mouse RAW264.7 cells MV\BdI9xfG:|aYOgZZN{[Xl? MVqxNFAhdk1? MXKxOkBp MlWzTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCuYYTlJIFxd3C2b4TpZ{Bk\WyuczDheEAyODBibl2gZYZ1\XJiMU[gbJJ{KHW|aX7nJIFvdmW6aX6gWk1xem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJ6KG[ub4egZ5l1d22ndIL5 M4X6TFE6OzB5M{W5
RAW 264.7 cells MVfGeY5kfGmxbjDhd5NigQ>? M1\NOlExOCCwTR?= MV;BcpRqdWmlcn;ibYFtKGGldHn2bZR6KGGpYXnud5QhW2GubX;u[Yxt[SCnboTldolk[SCWeYDobY12emm3bTCxOFAzQCCrbn\lZ5Rm\CCrbjDSRXchOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJIlv\mWldHXkJINmdGy|IHH0JFExOCCwTR?= MlvPNVk{ODd|NUm=
human H4 cells M4nwfGZ2dmO2aX;uJIF{e2G7 NXGyTlg6OC52IN88US=> M{ezRVI1KGh? M1XCc2lv\HWldHnvckBw\iCuaXfoeEBkcGGrbjCzMWdHWCCuZY\lcEBqdiCqdX3hckBJPCClZXzsd{BifCByLkSgeW0h[W[2ZYKgNlQhcHK|IHL5JIhq\2hidHjyc5VocHC3dDDmcJVwemW|Y3XuZ4UhdWmlcn;zZ49xgSC{ZXzheIl3\SC2bzDjc451em:u NUO0b4xNOThyMkS1PFQ>

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Bafilomycin A1 (1 μM and 0.1 μM) completely inhibits the resorptive activity of cultured osteoclasts. [8] Bafilomycin A1 dose-dependently inhibits the rate of Na+ uptake in young tilapia with a Ki of 0.16 μM. [9]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[2]

+ 展開

ATPase enzyme activity assays:

The ATPase enzyme assay medium contains 6 mM MgSO4, 50 mM HEPES (pH 7.4), 200 mM Na2SO3 (V-ATPase activator), 0.5 mM sodium ortho-vanadate (P-ATPase inhibitor), 0.5 mM sodium azide (F-ATPase inhibitor) and 3 mM Na2ATP. This medium (1.0 mL), with or without the addition of the V-type ATPase inhibitor bafilomycin A1, is incubated with the filtered homogenate (0.1 mL) for 60 minutes at 23–25 °C. The reaction is stopped by the addition of 1 mL of TCA 3%. Spectrometric blanks are prepared as for the enzyme assay with the exception that the tissue sample is added after the acid. Phosphate analysis is accomplished by adding 2 mL of 1-butanol and 0.2 mL molybdate solution (5 g ammonium molybdate, 22 mL H2SO4 to 100 mL). After vortexing for 15 seconds the solution is neutralised with 0.5 mL citrate solution (100 g/500 mL, pH 7.0) and again vortexed for 15 seconds. The solution is then centrifuged (2000 × g; 3 minutes) to separate the butanol phase and the absorbance of this phase is read at 400 nm. Standards of orthophosphate are prepared (0.1 μM–2.0 μM) and treated in the same way as the enzyme activity assays. Enzyme activity is expressed in μmol of orthophosphate liberated per hour and per milligram of protein. V-ATPase activity is considered to be the difference between the total ATPase activity measured in the presence of Na2SO3, sodium orthovanadate and sodium azide and the ATPase activity measured in the presence of these reagents and of the specific V-ATPases inhibitor Bafilomycin A1.
細胞試験:

[4]

+ 展開
  • 細胞株: HeLa cells
  • 濃度: ~20 nM
  • 反応時間: 20 hours
  • 実験の流れ:

    H. pylori bacteria extract is treated with inhibitors, before addition to HeLa cells, as follows: DCCD 10 mM for 1 hour at 30 ºC; NBD-CI 100 μM for 1 hour at 30 ºC and the reaction is blocked with glycine 10 mM final concentration; NEM 275 μM for 1 hour at 30 ºC and the reaction is blocked by addition of β-mercaptoethanol 275 mM; Mg-ATP 14 μM for 1 hour at 0 ºC; 100 μM KNO3 and 14 μM Mg-ATP for 1 hour at 30 μM; NaCO3 100 μM, pH 11 for 1 hour at 0 ºC. The bacterial extract is then added to cell with a 40-fold dilution at a final concentration of 0.65 mg/mL. Controls are HeLa cells incubated with untreated bacterial extracts and cells treated with inhibitor Bafilomycin A1 under the same conditions as bacterial extracts, at the same concentrations or after a 40-fold dilution. The vacuotating activity of the bacterial extracts is assayed.


    (参考用のみ)
動物試験:

[9]

+ 展開
  • 動物モデル: Young (approximately 9 days old) Mozambique tilapia, Oreochromis mossambicus
  • 製剤: Aquarium water containing 0.05 % dimethylsulphoxide (DMSO)
  • 投薬量: 10 μM
  • 投与方法: --
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 0.1 mg/mL (0.16 mM)
Ethanol 0.1 mg/mL (0.16 mM)
Water Insoluble

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 622.83
化学式

C35H58O9

CAS No. 88899-55-2
保管
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    How to dissolve it?

  • 回答:

    S1413 Bafilomycin A1(Baf-A1) is soluble in DMSO at 0.1 mg/ml. Please do not use alcohols as solvent, because this compound will degrade in alcohols.

Proton Pump信号経路図

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID