Y-27632 2HCl

製品コードS1049

Y-27632 2HCl化学構造

分子量(MW):320.26

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

サイズ 価格(税別)  
JPY 25896.00
JPY 11620.00
JPY 19920.00
JPY 28220.00
JPY 94620.00

文献中の使用例(50)

カスタマーフィードバック(6)

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

  • Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

製品安全説明書

ROCK阻害剤の選択性比較

生物活性

製品説明 Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
ターゲット
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外試験

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells M1XuVGZ2dmO2aX;uJGF{e2G7 M2HCc|ExKM7:TR?= NUXCTFhpOiCq M4jXWGROW09? MUfJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz MmnMPVY1PzZ3NB?=
N1E-115 NWW0c45zTnWwY4Tpc44hSXO|YYm= NVznTG4yOTBizszN M4fvRlIhcA>? MWjEUXNQ NF;Jd5FKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ M13J[|k3PDd4NUS=
HeLa M2nDWWZ2dmO2aX;uJGF{e2G7 NFeyZocyOCEQvF2= MVyzNEBucW5? MV3Jcohq[mm2czD0bIUh\m:{bXH0bY9vKG:oIIP0doV{eyCoaXLldpMh[W6mIITo[UBie3OnbXLsfUBw\iC4aX7jeYxqdi2lb370ZYlvcW6pIH\vZ4FtKGGmaHXzbY9vew>? M1HCZVk3PjhyN{K=
CCL39 MXjGeY5kfGmxbjDBd5NigQ>? MXOzNEDPxE1? NFTCV4w{OCCvaX6= MofnR49ueGyndHXsfUBi[m:uaYPo[ZMh[WO2aY\heIlwdiCxZjDOZU1JKGW6Y3jhcodmeiCQSFWxJIJ6KGmwdHXndolvew>? MmHCPVY6OzN6Mh?=
Mesothelial cells from rat mesentery NFfkOHVKdn[jc3n2[UBCe3OjeR?= NEnGVmE{OCEQvF2= M3\XdlIxKGh? NH71T5hDdG:la4OgbY53[XOrdnWgZYN1cX[rdIm= NV:wfXFJQTl|MEi3Ni=>
NIH3T3 M1KybGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYGxNEDPxE1? NHrEfJYyQCCm NXvnRWxqTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NEP2WnQyODB{MUO4Oi=>
Dbl-d MorNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGro[2QyOCEQvF2= NWXLXGE3OThiZB?= M3HaZXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NYC3VoFJOTByMkGzPFY>
Dbl-e M4r4SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW[xNEDPxE1? NVTwPFBWOThiZB?= NGDrRWhOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NXzKNWVEOTByMkGzPFY>
mNET1-d MnnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\rNVAh|ryP NG\TfHIyQCCm MmfhV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NHjtbpYyODB{MUO4Oi=>
mNET1-e NY[zRmt4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fG[VExKM7:TR?= Mn\INVgh\A>? M{XkeHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MUGxNFAzOTN6Nh?=
Ras-2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XHU|ExKM7:TR?= NWHlSohZOThiZB?= MkXOV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> M1niU|ExODJzM{i2
Ras-4 M4f2bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mon6NVAh|ryP Mn3rNVgh\A>? NILGVY5UfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MlrvNVAxOjF|OE[=
Src-1 NYjSO2ZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWSxNEDPxE1? NFjCUm0yQCCm MYrEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MX[xNFAzOTN6Nh?=
Src-4 NULvdnRrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTaNVAh|ryP MXKxPEBl NUHaeY84TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M2PRVVExODJzM{i2
NIH3T3 M4XibGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjp[GpXOTBizszN M13jPFE5KGR? M1HUOWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MkO1NVAxOjF|OE[=
Src-1 NFjuVYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVyxNEDPxE1? M2rMSFE5KGR? MUjEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To M17HSFExODJzM{i2
Src-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PZSlExKM7:TR?= NX[wdmkzOThiZB?= MlSySI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NXPQPZBHOTByMkGzPFY>
SW620 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1r3WVExKM7:TR?= NVPLdFd3OThiZB?= NWnaTXRoTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NX7u[ohqOTByMkGzPFY>
HCT15 NUfXXYFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3mWVdiOTBizszN MVuxPEBl NIrXcFFFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MlnHNVAxOjF|OE[=
HCT116 NYjwdlVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXEb4IyOCEQvF2= M3XyO|E5KGR? NV7CNHdIW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? MYGxNFAzOTN6Nh?=
LS174T MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\lNVAh|ryP NXfoW3FqOThiZB?= M1TjT21w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? MVqxNFAzOTN6Nh?=
Neonatal rat ventricular myocytes MVHGeY5kfGmxbjDBd5NigQ>? NXrUPJVwOTBizszN NGHQWZU1QCCq NXnFWHh{UW6qaXLpeJMhTVRvMT3pcoR2[2WmIHnuZ5Jm[XOnczDpckBxem:2ZXnuJJN6dnSqZYPpd{wh[2WubDDzbZpmKGGwZDDtfY9ncWK{aXzsZZIhd3KpYX7pfoF1cW:w NUPGXWpvOTB|OE[2NVM>
Stellate Cell NF70fXVHfW6ldHnvckBCe3OjeR?= M{CzZ|I2KM7:TR?= M3rVXFE2KG2rbh?= MmDiTY5pcWKrdIOg[o9zdWG2aX;uJI9nKEZvYXP0bY4he3S{ZYPzJIZq[mW{czDhcoQheGixc4Doc5J6dGG2aX;uJI9nKG27b4PpckBtcWeqdDDjbIFqdg>? NFzoV|YyODZyMES5Oi=>
Rat Vascular Smooth Muscle Cells M{XrUWZ2dmO2aX;uJGF{e2G7 NH\wWpoyOCEQvF2= MWeyJIg> MmHXTY5pcWKrdIOgZY5ocW:2ZX7zbY4hUUlvaX7keYNm\CCqeYDldpRzd3CqeR?= NHfWemcyODZ2MkOxOy=>
PC3 MUPGeY5kfGmxbjDBd5NigQ>? MXuyOUDPxE1? NIHiW|gyKGh? NELiTWVKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? M4Tu[FExPzJyNEex
PC3 M2nNcG1q\3KjdHnvckBCe3OjeR?= NULje2Z1OjVizszN M3H1N|EhcA>? MUTJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= NFi3PFkyODd{MES3NS=>
PC3 M4LUeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\ke5YzPSEQvF2= NVnScJczOTdiaB?= NHnhVHNFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MmHWNVA4OjB2N{G=
LNCaP NXTOPHRwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGC2eVUzPSEQvF2= MYOxO{Bp NWXhVmZpTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MYmxNFczODR5MR?=
Rat hepatic stellate cells MYDGeY5kfGmxbjDBd5NigQ>? MlvVN|Ah|ryP NUDyc5RGPDhiaB?= MlHTSIlucW6rc3jld{B1cGVicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb|ItKGGwZDDk[YNz\WG|ZYOgcoV4KESQQTDzfY51cGW|aYO= MorsNVA5PDV4NkO=
Pancreatic acinar cells MlLPSpVv[3Srb36gRZN{[Xl? M{n0UVExyqEQvF2= MX63NEBucW5? MmrkVI91\W62aXH0[ZMhS0ONLYP0bY12dGG2ZXSgdIFv[3KnYYTpZ{Bmdnq7bXWgd4VkemW2aX;u Mmi4NVI4PDVyOEC=
C2C12 M33vWmZ2dmO2aX;uJGF{e2G7 NHjEPFIyOMLizszN NHS3S4Q3KGh? MYTQdoV3\W62czD0bIUhe2W{aX7lJJBpd3OyaH;yfYxifGmxbjDv[kBKWlNvMTDpcoR2[2WmIHL5JIlve3WuaX6gZY5lN2:{IGTOSk3PuQ>? NIPQb3AyPjJ4N{GyOC=>
PC 12 NXzqSI53TnWwY4Tpc44hSXO|YYm= MXyxNOKh|ryP MXGyOEBp NYHncIpWSXS2ZX71ZZRmeyClYYTlZ4hwdGGvaX7lJIJqd3O7boTo[ZNqew>? NXPRR3RqOTZ{MUm0NlQ>
Cynomolgus monkey embryonic stem cells M2SzOGN6fG:2b4jpZ{BCe3OjeR?= MWGyNEDDvU1? NHP4bFAzPCCq MmDDVJJwdW:2ZYOgZ5lGWyClZXzsJJN2en[rdnHs NV\jcIM4OTh7NEC4OVU>
TSGH 8301 MljCUYloemG2aX;uJGF{e2G7 MluzNlAhyrWP MX2xJIg> MkPBTY5kemWjc3XzJINmdGxibXnndoF1cW:w NIexfYUyQTh7NkS3OS=>
Swiss3T3 NGDZTIhEd2yxbomt[o9zdWmwZzDBd5NigQ>? NVXMfXpwOTBiwsXN M{\5SVE{KGR? MmfvTY5kemWjc3XzJJBzd3O2YYTlJINmdGxiY3;sc456NW[xcn3pcoch[WO2aY\peJk> MVqyNVQ3PDlyMh?=
HT22 MlG3R5l1d3SxeHnjJGF{e2G7 MkHBNVAhyrWP NXK4dGJlOTNiaB?= M4i1PXBzd3SnY4TzJIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgcoV2em:wYXyg[IVifGh? NFPGe5ozOjhzMEizOS=>
Salivary gland stem cells NEjPSYZHfW6ldHnvckBCe3OjeR?= NGPoNJAyOCEEtV2= NFHvclg4KGR? M2DXdHJm\HWlZYOgV2dUSyC|ZX7ld4NmdmOn NYLWXG1WOjV6MES1OlA>

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

体内試験 Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

お薦めの試験操作(参考用のみ)

動物試験:[1] [7]
+ 展開
  • 動物モデル: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • 製剤: Dissolved in DMSO, and diluted in saline (Rat); 0.9% NaCl (Mice)
  • 投薬量: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • 投与方法: Orally (Rat); i.p. (Mice)
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 64 mg/mL (199.83 mM) warming
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
saline
混合させたのち直ちに使用することを推奨します。
10mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 320.26
化学式

C14H21N3O.2HCl

CAS No. 129830-38-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 質問2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID