Y-27632 2HCl

製品コードS1049

Y-27632 2HCl化学構造

分子量(MW):320.26

Y-27632 2HClは一種の選択性的なROCK1 (p160ROCK)阻害剤で、無細胞試験でKi値が140 nMですが、ROCK1に作用する選択性は他のキナーゼ(PKC、cAMP依頼性タンパク質キナーゼ、,MLCKとPAKを含める)に作用する選択性より200倍以上が高くなります。

サイズ 価格(税別) 在庫  
JPY 25896.00 あり
JPY 11620.00 あり
JPY 19920.00 あり
JPY 28220.00 あり
JPY 94620.00 あり

文献中の使用例(46)

カスタマーフィードバック(5)

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

製品安全説明書

ROCK阻害剤の選択性比較

生物活性

製品説明 Y-27632 2HClは一種の選択性的なROCK1 (p160ROCK)阻害剤で、無細胞試験でKi値が140 nMですが、ROCK1に作用する選択性は他のキナーゼ(PKC、cAMP依頼性タンパク質キナーゼ、,MLCKとPAKを含める)に作用する選択性より200倍以上が高くなります。
ターゲット
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外試験

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells NIm0fXlHfW6ldHnvckBCe3OjeR?= NV7YNoNGOTBizszN M1fVTFIhcA>? NVzl[GlqTE2VTx?= NUm3[JV1UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? MmPrPVY1PzZ3NB?=
N1E-115 NULGc4x3TnWwY4Tpc44hSXO|YYm= NGr3boIyOCEQvF2= NGrrO2UzKGh? NV[0dXVOTE2VTx?= NGi4eopKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ M{L0Olk3PDd4NUS=
HeLa M4Sz[mZ2dmO2aX;uJGF{e2G7 M2TsRlExKM7:TR?= MWWzNEBucW5? MWLJcohq[mm2czD0bIUh\m:{bXH0bY9vKG:oIIP0doV{eyCoaXLldpMh[W6mIITo[UBie3OnbXLsfUBw\iC4aX7jeYxqdi2lb370ZYlvcW6pIH\vZ4FtKGGmaHXzbY9vew>? MV25OlY5ODd{
CCL39 MWLGeY5kfGmxbjDBd5NigQ>? MW[zNEDPxE1? NE\lW4E{OCCvaX6= NVvJfId5S2:vcHzleIVtgSCjYn;sbZNp\XNiYXP0bZZifGmxbjDv[kBP[S2KIHX4Z4hidmencjDOTGUyKGK7IHnueIVoemmwcx?= MXu5Olk{Ozh{
Mesothelial cells from rat mesentery MXLJcpZie2m4ZTDBd5NigQ>? NEDqWWQ{OCEQvF2= MV[yNEBp NF22coJDdG:la4OgbY53[XOrdnWgZYN1cX[rdIm= MkT4PVk{ODh5Mh?=
NIH3T3 M2mwWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLpNVAh|ryP MkXyNVgh\A>? NUfzbYlwTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M2fQdlExODJzM{i2
Dbl-d MlrwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjuRZMyOCEQvF2= NEnDWpYyQCCm NWXIWJBOW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NXvqN4lJOTByMkGzPFY>
Dbl-e MkX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUSxNEDPxE1? MUexPEBl NHju[WNOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NHGz[YcyODB{MUO4Oi=>
mNET1-d MoPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFu5TmcyOCEQvF2= MV6xPEBl NIHyd4NUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MYKxNFAzOTN6Nh?=
mNET1-e M3nGNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXTNVAh|ryP M3fIOlE5KGR? MXLTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NHPVeXcyODB{MUO4Oi=>
Ras-2 NV\T[|ZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{X2S|ExKM7:TR?= MYexPEBl MVfTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> M3KxOlExODJzM{i2
Ras-4 M1vqNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV[xNEDPxE1? NYHRZZpVOThiZB?= M{PUV3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M{jBXlExODJzM{i2
Src-1 NYTEPJROT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGH5PXEyOCEQvF2= NW\YV492OThiZB?= M13LUWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NHeyO4syODB{MUO4Oi=>
Src-4 Mn\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnX3NVAh|ryP NEnkV4QyQCCm NH\YZWVFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MUexNFAzOTN6Nh?=
NIH3T3 NUnlWHBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPsVmJkOTBizszN NXrjV3RMOThiZB?= MWDEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MoLqNVAxOjF|OE[=
Src-1 NUL4dYE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXvUox4OTBizszN NHfv[5oyQCCm MkDWSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NFjFO5UyODB{MUO4Oi=>
Src-2 NYfrTFV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvLNVAh|ryP MX[xPEBl M2PjOGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MVexNFAzOTN6Nh?=
SW620 M17IN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWKxNEDPxE1? NYO3SVhqOThiZB?= M{\LXGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NXTReHdnOTByMkGzPFY>
HCT15 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\NOlExKM7:TR?= MnX1NVgh\A>? MmXwSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NGHDcpoyODB{MUO4Oi=>
HCT116 M3;USmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[xNEDPxE1? NF71T2QyQCCm M4XzXXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MX2xNFAzOTN6Nh?=
LS174T NX\oO3JHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTQOGlSOTBizszN NIjKcmMyQCCm NF3t[2lOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq MYKxNFAzOTN6Nh?=
Neonatal rat ventricular myocytes MX\GeY5kfGmxbjDBd5NigQ>? M{jYWFExKM7:TR?= MUW0PEBp NUixPINkUW6qaXLpeJMhTVRvMT3pcoR2[2WmIHnuZ5Jm[XOnczDpckBxem:2ZXnuJJN6dnSqZYPpd{wh[2WubDDzbZpmKGGwZDDtfY9ncWK{aXzsZZIhd3KpYX7pfoF1cW:w NVzte4hEOTB|OE[2NVM>
Stellate Cell NX\4d2w{TnWwY4Tpc44hSXO|YYm= M4\rVVI2KM7:TR?= M2PCSFE2KG2rbh?= NELkfGVKdmirYnn0d{Bnd3KvYYTpc44hd2ZiRj3hZ5RqdiC|dILld5Mh\mmkZYLzJIFv\CCyaH;zdIhwenmuYYTpc44hd2ZibYnvd4lvKGyrZ3j0JINp[Wmw M{LYPFExPjByNEm2
Rat Vascular Smooth Muscle Cells NVvudoNvTnWwY4Tpc44hSXO|YYm= NFO3WYwyOCEQvF2= MomyNkBp NXLWPVNZUW6qaXLpeJMh[W6paX;0[Y5{cW5iSVmtbY5lfWOnZDDofZBmenS{b4DofS=> M3v3XlExPjR{M{G3
PC3 M1fPc2Z2dmO2aX;uJGF{e2G7 M3rTXFI2KM7:TR?= MoD4NUBp Mlz6TY5lfWOnczDtc5JxcG:ub3fpZ4FtKGOqYX7n[ZM> NVPVcmJiOTB5MkC0O|E>
PC3 M1Kycm1q\3KjdHnvckBCe3OjeR?= MnK1NlUh|ryP MkfsNUBp MoO2TY5pcWKrdIOgeIhmKEKPRlKtR20h[W6mIITo[UBGT0Zvc4TpcZVt[XSnZDDtbYdz[XSrb36= M1zUN|ExPzJyNEex
PC3 NEf4NWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkH4NlUh|ryP M{nINVE4KGh? NU\sVXZmTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M3rCblExPzJyNEex
LNCaP NUfueVMxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4X0PFI2KM7:TR?= M3HZbFE4KGh? M{Hzc2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M4Hr[lExPzJyNEex
Rat hepatic stellate cells NHHTS4pHfW6ldHnvckBCe3OjeR?= MnrhN|Ah|ryP MkK2OFghcA>? NXq4bm5XTGmvaX7pd4hmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzczJuIHHu[EBl\WO{ZXHz[ZMhdmW5IFTORUB{gW62aHXzbZM> NGrtO5kyODh2NU[2Ny=>
Pancreatic acinar cells NGrkfXNHfW6ldHnvckBCe3OjeR?= M1HWSVExyqEQvF2= M2PEblcxKG2rbh?= NGfRV2ZRd3SnboTpZZRmeyCFQ1utd5RqdXWuYYTl[EBx[W6lcnXheIlkKGWweont[UB{\WO{ZYTpc44> NFWzc2QyOjd2NUC4NC=>
C2C12 NW\FPXJQTnWwY4Tpc44hSXO|YYm= NFG1U5AyOMLizszN M{\kZ|YhcA>? NUTvUZpJWHKndnXueJMhfGinIIPldolv\SCyaH;zdIhwenmuYYTpc44hd2ZiSWLTMVEhcW6mdXPl[EBjgSCrboP1cIlvKGGwZD;vdkBVVkZvzsG= NWLjcFg2OTZ{NkexNlQ>
PC 12 MmnOSpVv[3Srb36gRZN{[Xl? NGTyXGUyOMLizszN M{PJXVI1KGh? MXLBeJRmdnWjdHXzJINifGWlaH;sZY1qdmViYnnvd5lvfGinc3nz M{LjTlE3OjF7NEK0
Cynomolgus monkey embryonic stem cells M2fqe2N6fG:2b4jpZ{BCe3OjeR?= MVqyNEDDvU1? NEWybmIzPCCq M2LubnBzd22xdHXzJIN6TVNiY3XscEB{fXK4aY\hcC=> M4HjPVE5QTRyOEW1
TSGH 8301 MXPNbYdz[XSrb36gRZN{[Xl? M1HLb|IxKML3TR?= MX[xJIg> MmPETY5kemWjc3XzJINmdGxibXnndoF1cW:w MYSxPVg6PjR5NR?=
Swiss3T3 MUfDc4xwdnlvZn;ycYlv\yCDc4PhfS=> MUGxNEDDvU1? MnrlNVMh\A>? M3nuU2lv[3KnYYPld{Bxem:|dHH0[UBk\WyuIHPvcI9vgS2ob4LtbY5oKGGldHn2bZR6 NX;CN|l5OjF2NkS5NFI>
HT22 M1Lk[GN6fG:2b4jpZ{BCe3OjeR?= M3;sR|ExKML3TR?= NEfnRowyOyCq MVvQdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIH7leZJwdmGuIHTlZZRp NGnu[oszOjhzMEizOS=>
Salivary gland stem cells MlvSSpVv[3Srb36gRZN{[Xl? MVGxNEDDvU1? NYXPXHBXPyCm MV3S[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? MXOyOVgxPDV4MB?=

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

お薦めの試験操作(参考用のみ)

動物試験:[1] [7]
+ 展開
  • 動物モデル: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • 製剤: Dissolved in DMSO, and diluted in saline (Rat); 0.9% NaCl (Mice)
  • 投薬量: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • 投与方法: Orally (Rat); i.p. (Mice)
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 64 mg/mL (199.83 mM) warming
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 順序で溶剤を入れること:
saline
10mg/mL

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 320.26
化学式

C14H21N3O.2HCl

CAS No. 129830-38-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 問題2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK信号経路図

ROCK Inhibitors with Unique Features

相関ROCK製品

Tags: Y-27632 2HClを買う | Y-27632 2HCl ic50 | Y-27632 2HCl供給者 | Y-27632 2HClを購入する | Y-27632 2HCl費用 | Y-27632 2HCl生産者 | オーダーY-27632 2HCl | Y-27632 2HCl化学構造 | Y-27632 2HCl分子量 | Y-27632 2HCl代理店
×
細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID