Acetylcysteine (N-acetylcysteine)

別名:N-acetylcysteine

Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions are unstable and should be fresh-prepared.

Acetylcysteine (N-acetylcysteine)化学構造

CAS No. 616-91-1

サイズ 価格(税別) 在庫状況
JPY 12000 国内在庫あり
JPY 17900 国内在庫あり
JPY 26100 国内在庫あり

代表番号: 045-509-1970|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(171)

製品安全説明書

現在のバッチを見る: 純度: 99.97%
99.97

Acetylcysteine (N-acetylcysteine)と併用されることが多い化合物

Ciprofloxacin


Acetylcysteine and Ciprofloxacin therapy is used as an alternative therapeutic option for Partial Biliary Obstruction.

Ozdil B, et al. J Clin Pharmacol. 2010 Dec;50(12):1414-9.

Tigecycline


Acetylcysteine and Tigecycline significantly decrease all viable biofilm-associated bacteria.

Aslam S, et al. Antimicrob Agents Chemother. 2007 Apr; 51(4): 1556–1558.

Prostaglandin E2 (PGE2)


Acetylcysteine and Prostaglandin E2 synergistically reduce apoptosis, improve liver enzymes, and prevent death.

North TE, et al. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17315-20.

Colistin


Acetylcysteine and Colistin exhibit in vitro synergism against Stenotrophomonas maltophilia.

Ciacci N, et al. Antibiotics (Basel). 2019 Jul 25;8(3):101.

Acetylcysteine (N-acetylcysteine)関連製品

シグナル伝達経路

TNF-alpha阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
HUVEC cells Function assay 2μM,4μM,8μM 90min HUVECs that were exposed with serum of P. falciparum and treated with NAC 2 µM,4 µM,8 µM. Supernatant from culture and lysed cells culture were measured for H2O2, GSH and MDA levels. 21602763
HUVEC cells Function assay 10μM 2h HUVECs were pretreated with 10 μM NAC for 2 hours, and then treated with 0.5 μM PCB 118. presentce of NAC for 48 hours. 28592194
NHBE Cytotoxicity assay 1 mM 18 hrs Cytoprotective activity in NHBE cells assessed as inhibition of tBHP-induced GSH depletion at 1 mM preincubated for 18 hrs followed by tBHP addition for 4 hrs by thiostar dye based fluorescence assay 27031670
HUVEC Antioxidant assay 5 mmol/L 4 hrs Antioxidant activity against H2O2-induced lipid accumulation in HUVEC cells assessed as reduction in MDA level at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs 22841280
HUVEC Antioxidant assay 5 mmol/L 4 hrs Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs 22841280
HUVEC Cytotoxicity assay 5 mmol/L 4 hrs Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay 22841280
HUVEC Cytotoxicity assay 5 mmol/L 4 hrs Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as LDH release at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by LDH assay 22841280
SH-SY5Y Neuroprotective assay 5 mM 4 hrs Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as upregulation of Bax expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis 23403085
SH-SY5Y Neuroprotective assay 5 mM 4 hrs Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as downregulation of Bcl2 expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis 23403085
HT22 Neuroprotective assay 5 mM 24 hrs Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as increase in cell viability at 5 mM after 24 hrs by MTT assay 29122481
HT22 Neuroprotective assay 50 uM 10 to 12 hrs Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as reduction in apoptotic cells at 50 uM after 10 to 12 hrs by Annexin V-alexa 488/propidium iodide staining based flow cytometry 29122481
HL-7702 Hepatoprotective assay 10 uM 24 hrs Hepatoprotective activity against APAP-induced cell injury in human HL-7702 cells assessed as increase in survival rate at 10 uM pre-incubated for 24 hrs before APAP addition and measured 6 hrs post APAP challenge by MTT assay 28729056
PC12 Cytotoxicity assay 24 hrs Inhibition of 6-hydroxydopamine induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level 17158454
BL21 (DE3) Function assay 30 mins Inhibition of hexahistidine-tagged IMP-7 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using fluorocillin as substrate incubated for 30 mins by TECAN fluorescent plate reader analysis, IC50 = 20.7 μM. 25815530
PC12 Cytotoxicity assay 24 hrs Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level 17158454
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明 Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions are unstable and should be fresh-prepared.
Targets
NF-κB [8] Ferroptosis [9] ROS [6] TNF-α [7]
In Vitro
In vitro

N-acetylcysteine inhibits activation of c-Jun N-terminal kinase, p38 MAP kinase and redox-sensitive activating protein-1 and nuclear factor kappa B transcription factor activities regulating expression of numerous genes. N-acetylcysteine can also prevent apoptosis and promote cell survival by activating extracellular signal-regulated kinase pathway, a concept useful for treating certain degenerative diseases. N-acetylcysteine directly modifies the activity of several proteins by its reducing activity. [1]

N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents death of PC12 cells and sympathetic neurons. [2]

N-acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells. [3]

N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it was suggested that NAC interferes with redox-sensitive steps in the NGF mechanism. [4]

細胞実験 細胞株 MCF cells
濃度 5 mM
反応時間 24 h
実験の流れ

Cells were treated with indicated concentration of drug for 24 h.

In Vivo
In Vivo

N-acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight. [5]

動物実験 動物モデル Male SAMP8 mice
投与量 100 mg/kg
投与経路 s.c.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06112834 Not yet recruiting
Botulism
California Department of Public Health
June 2024 Phase 2
NCT04520139 Not yet recruiting
Ovarian Cancer|Cognitive Impairment
University of California Irvine|Jarrow Formulas Inc
December 2023 Phase 1|Phase 2
NCT06039280 Not yet recruiting
Hepatocellular Carcinoma
Institute of Liver and Biliary Sciences India
September 15 2023 Not Applicable

化学情報

分子量 163.19 化学式

C5H9NO3S

CAS No. 616-91-1 SDF Download Acetylcysteine (N-acetylcysteine) SDFをダウンロードする
Smiles CC(=O)NC(CS)C(=O)O
保管 3 years -20°C powder 溶液状態は不安定なので使用直前に調整してください。少量づつ分包して保管し、都度使い切る事が推奨されます。

In vitro
Batch:

DMSO : 32 mg/mL ( (196.09 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 32 mg/mL

Ethanol : 32 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください
Tags: Acetylcysteine (N-acetylcysteine)を買う | Acetylcysteine (N-acetylcysteine) ic50 | Acetylcysteine (N-acetylcysteine)供給者 | Acetylcysteine (N-acetylcysteine)を購入する | Acetylcysteine (N-acetylcysteine)費用 | Acetylcysteine (N-acetylcysteine)生産者 | オーダーAcetylcysteine (N-acetylcysteine) | Acetylcysteine (N-acetylcysteine)化学構造 | Acetylcysteine (N-acetylcysteine)分子量 | Acetylcysteine (N-acetylcysteine)代理店