Afatinib (BIBW2992)

製品コードS1011

Afatinib (BIBW2992)化学構造

分子量(MW):485.94

Afatinib (BIBW2992)はEGFR/HER2( EGFR(wt)、EGFR(L858R)、EGFR(L858R/T790M) とHER2を含める)を不可逆に抑制して、無細胞試験でIC50値が0.5 nM,0.4 nM,10 nM 和 14 nMにそれえぞれ分かれることです。

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文献中の引用(37)

カスタマーフィードバック(4)

  • Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Afatinib (BIBW2992)はEGFR/HER2( EGFR(wt)、EGFR(L858R)、EGFR(L858R/T790M) とHER2を含める)を不可逆に抑制して、無細胞試験でIC50値が0.5 nM,0.4 nM,10 nM 和 14 nMにそれえぞれ分かれることです。
ターゲット
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 10 nM 14 nM
体外試験

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 NUn5e2F5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTBwMEC1O|Ih|ryP NWXyb|JrW0GQR1XS
KASUMI-1 Mo\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEC5NVEh|ryP NYjpS3AxW0GQR1XS
HCC2218 MlnCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fhOWlEPTB;MD6wNFk5PyEQvF2= NWfQdG4xW0GQR1XS
KARPAS-45 NX7BfVJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTlTWM2OD1yLkCxOlcyKM7:TR?= NF7te3BUSU6JRWK=
HSC-4 NGjLVJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\0TWM2OD1yLkCxO|Q5KM7:TR?= NWPjW41iW0GQR1XS
CHL-1 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkD6TWM2OD1yLkCxPFM6KM7:TR?= M4\JPXNCVkeHUh?=
KY821 MkfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTYenlKSzVyPUCuNFIxPiEQvF2= NGLjWHJUSU6JRWK=
NCI-N87 MmjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3CZ5hKSzVyPUCuNFI4PzNizszN M4jBT3NCVkeHUh?=
DOK NG[wfFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTBwMEOxPVQh|ryP NYq2U2QyW0GQR1XS
DSH1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33FSmlEPTB;MD6wN|MyPCEQvF2= MoXKV2FPT0WU
MDA-MB-175-VII M3HtWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTBwMEO0NVYh|ryP NXjteHJqW0GQR1XS
NCI-H2170 MoDuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTBwMEO3Nlgh|ryP NEDucWxUSU6JRWK=
BT-474 M3\CUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TTNWlEPTB;MD6wOFk5PiEQvF2= MXTTRW5ITVJ?
EFO-27 MkjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwMEWxPFIh|ryP Mn3IV2FPT0WU
NCI-H720 M{nKbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEC5NWVKSzVyPUCuNFU{OjVizszN NETNcldUSU6JRWK=
PC-14 NX3SXGdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;JUFdkUUN3ME2wMlA2PjZ6IN88US=> MXvTRW5ITVJ?
EC-GI-10 NYjSVnNJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTBwMEW5NVIh|ryP NIXCTINUSU6JRWK=
KYSE-450 MnrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTBwMEW5O|gh|ryP MlmyV2FPT0WU
BPH-1 NIHlc4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPMZZFPUUN3ME2wMlA2QTh6IN88US=> NWTDN|VKW0GQR1XS
NCI-H292 M2\E[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTBwME[wPUDPxE1? MXPTRW5ITVJ?
H9 M2D0XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMEexN|Ih|ryP M3u1NHNCVkeHUh?=
NCI-H526 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXqzWFRqUUN3ME2wMlA4QDJ|IN88US=> MoHQV2FPT0WU
NCI-H1623 M3T5UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwMEiwNlUh|ryP MnS0V2FPT0WU
KYSE-140 NIexV2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLZTWM2OD1yLkC4N|Mh|ryP MkSzV2FPT0WU
Ca9-22 MlX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTBwMEizOVMh|ryP MYnTRW5ITVJ?
AU565 NUDIdo5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\QR2lEPTB;MD6wPFUxPCEQvF2= NECyO3ZUSU6JRWK=
ZR-75-30 M2PQbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7ofGtKSzVyPUCuNFk2PDVizszN NITjRZVUSU6JRWK=
NCI-H1770 MmrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmn5TWM2OD1yLkGwNlgh|ryP M3rkRnNCVkeHUh?=
LB2241-RCC MnTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYflN4JxUUN3ME2wMlExQDh2IN88US=> M2W5TXNCVkeHUh?=
CAL-39 NXHL[JNnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzRS29IUUN3ME2wMlEyPzl{IN88US=> NUC4d4NVW0GQR1XS
UACC-893 MlPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEj2b4xKSzVyPUCuNVE5QDVizszN MWLTRW5ITVJ?
LU-65 M3y1S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zYTmlEPTB;MD6xNlY{OyEQvF2= MWfTRW5ITVJ?
HN M2fvRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrCRYR4UUN3ME2wMlE{ODd5IN88US=> Mnu4V2FPT0WU
TI-73 M3y0XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETTSGtKSzVyPUCuNVQ4OTVizszN NWHiSWpuW0GQR1XS
KU812 M4PnWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4roWWlEPTB;MD6xOVQ2PiEQvF2= NVG5cWFDW0GQR1XS
SW13 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\TVYRKSzVyPUCuNVYzOTZizszN MXPTRW5ITVJ?
BB30-HNC MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMU[1O|Yh|ryP M2\nRnNCVkeHUh?=
HSC-2 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTBwMU[4N|ch|ryP NWTrS5drW0GQR1XS
BV-173 NHLoTpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjYTWM2OD1yLkG3OVk3KM7:TR?= Mlu0V2FPT0WU
CAL-27 NXPSTm9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVz5PI13UUN3ME2wMlE4PjN4IN88US=> M1fIUHNCVkeHUh?=
SBC-1 NXra[Ho1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPWTWM2OD1yLkG4PVU6KM7:TR?= MXPTRW5ITVJ?
CAL-33 NIezS4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXrTWM2OD1yLkKwNVg1KM7:TR?= NF;yS4hUSU6JRWK=
NCI-H209 M1LufGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTBwMkC0OkDPxE1? M2HQVXNCVkeHUh?=
COR-L105 NIO3VHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGH3ZmxKSzVyPUCuNlExQDRizszN MoX6V2FPT0WU
HSC-3 M2LkO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkP2TWM2OD1yLkKxNVk5KM7:TR?= MX\TRW5ITVJ?
NCI-H69 MmT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DSbmlEPTB;MD6yNVQ2OSEQvF2= NYnsRXlCW0GQR1XS
MOLT-13 NHzaU2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrkVZlKSzVyPUCuNlM6PzVizszN MXzTRW5ITVJ?
ML-2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUniOVI4UUN3ME2wMlI1QDB{IN88US=> MUTTRW5ITVJ?
IGROV-1 NVLuV5M2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLaTWM2OD1yLkK1PVUzKM7:TR?= NGnOeYlUSU6JRWK=
TK10 NGPLfVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF6wXohKSzVyPUCuNlY4OzhizszN NYL0[XJXW0GQR1XS
EoL-1-cell NHezd2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LE[GlEPTB;MD6yOlg6OiEQvF2= MUDTRW5ITVJ?
HO-1-N-1 NWjrO4FYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37YXWlEPTB;MD6yO|g2OyEQvF2= M1vKTXNCVkeHUh?=
BHY NIHuSIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\pcYRKSzVyPUCuNlg3PTVizszN MYrTRW5ITVJ?
LU-139 M1\uTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzHTWM2OD1yLkOwOVEh|ryP NELoV2dUSU6JRWK=
23132-87 NIKxUnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnXTWM2OD1yLkOxPVI{KM7:TR?= NYTKPYprW0GQR1XS
ACHN M4PPdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfJRYMzUUN3ME2wMlMyQTl2IN88US=> MlG1V2FPT0WU
DoTc2-4510 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWqxPGVXUUN3ME2wMlMzOjF7IN88US=> M{HYVXNCVkeHUh?=
BB65-RCC MmXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwM{S1PVMh|ryP M3\ydnNCVkeHUh?=
HLE NFO2blJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDYendKSzVyPUCuN|UxPDZizszN NYPXNYNzW0GQR1XS
HCC1419 Mon6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz3TWM2OD1yLkO2OFA5KM7:TR?= M1Kze3NCVkeHUh?=
A388 NV3H[|h6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrSc45KSzVyPUCuN|Y5PTdizszN NXnHWnBRW0GQR1XS
NUGC-3 MkS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\tdW16UUN3ME2wMlM5Ozd|IN88US=> MVLTRW5ITVJ?
KYSE-180 NE\aTGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwM{mwPFkh|ryP M1\LeXNCVkeHUh?=
TE-6 M2TnTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDOeZU4UUN3ME2wMlQxOjR4IN88US=> M4DB[nNCVkeHUh?=
A498 NIKybnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnqTWM2OD1yLkSwOlc1KM7:TR?= M4XGOHNCVkeHUh?=
NCI-H1648 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwNESxPFIh|ryP NELaeGpUSU6JRWK=
DU-145 NVXBdYZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXX5V|BzUUN3ME2wMlQ1QDN|IN88US=> NVvTPJdjW0GQR1XS
CW-2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXuW2p7UUN3ME2wMlQ3QTF2IN88US=> Mn;aV2FPT0WU
A431 M3fycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofpTWM2OD1yLkS4NlM{KM7:TR?= M3vHVHNCVkeHUh?=
SAS M3:2c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPVZm8xUUN3ME2wMlQ5Ozl6IN88US=> NIDNN4tUSU6JRWK=
NCI-H2126 M1;jdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrweVdZUUN3ME2wMlUyQTJ4IN88US=> MlqxV2FPT0WU
NCI-H358 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nJc2lEPTB;MD61N|IyKM7:TR?= MUPTRW5ITVJ?
FADU M2e1[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXETWM2OD1yLkW0OFA1KM7:TR?= NWjx[3FrW0GQR1XS
ACN NYL0XohlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;aNGlEPTB;MD61OVA6PCEQvF2= M2nhSXNCVkeHUh?=
HCC70 Mnj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTtTWM2OD1yLkW3OlYyKM7:TR?= MlnVV2FPT0WU
NCI-H1838 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\PUYpJUUN3ME2wMlYxOjl7IN88US=> Mlz5V2FPT0WU
CAL-54 Ml7wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwNkG2NlEh|ryP NGq5d|FUSU6JRWK=
NCI-H1975 M1nodWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rZOWlEPTB;MD62NVczQCEQvF2= Mk\JV2FPT0WU
COR-L88 MkfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIryfGFKSzVyPUCuOlQyOTNizszN NUXTUHdMW0GQR1XS
CTV-1 MlLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTBwNkSzPVIh|ryP MXnTRW5ITVJ?
EW-18 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrpS3ZKSzVyPUCuOlg3QThizszN MV;TRW5ITVJ?
HCC1806 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\oTWM2OD1yLk[5OlY3KM7:TR?= M4\SdnNCVkeHUh?=
HuP-T4 NUiz[mZ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\0T3dJUUN3ME2wMlY6PzZizszN MUjTRW5ITVJ?
MDA-MB-361 Mn3pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTBwN{K2OlIh|ryP NWf6bmV5W0GQR1XS
NCI-H1573 M1n5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnRfXdVUUN3ME2wMlc{PjNizszN NF70eFlUSU6JRWK=
KU-19-19 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV70VVVHUUN3ME2wMlc4PDZ2IN88US=> NWXwOXMzW0GQR1XS
COLO-678 NInRPXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;WTWM2OD1yLkiwPFM1KM7:TR?= M{T3R3NCVkeHUh?=
C-33-A NHG2T|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XET2lEPTB;MD64NVI6PyEQvF2= MlXPV2FPT0WU
Calu-3 M1r1WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DJXWlEPTB;MD64NVQ{PCEQvF2= Mn;GV2FPT0WU
AGS Mn7JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\P[2lEPTB;MD64N|Y1QCEQvF2= M{fEbHNCVkeHUh?=
SCC-25 NILteoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTBwOESxO|Eh|ryP NV7FRlVIW0GQR1XS
JVM-2 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwOEiyPVkh|ryP MmT3V2FPT0WU
HCE-T NHjqeHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\1ZmV1UUN3ME2wMlg5PDJ6IN88US=> M37wTHNCVkeHUh?=
ES4 NGKzU4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTBwOUe4N|Yh|ryP MnHtV2FPT0WU
EKVX NXzGSlJYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIG3S5dKSzVyPUGuNFExPjRizszN NV;h[FB6W0GQR1XS
D-542MG MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1GzVWlEPTB;MT6wNVA3PCEQvF2= MX;TRW5ITVJ?
SW1116 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTFwMEGyPFch|ryP MYXTRW5ITVJ?
5637 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXT1VlNQUUN3ME2xMlAyPTFizszN NE\RTm9USU6JRWK=
HT-1376 M2DUXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXSTWM2OD1zLkCxOVE{KM7:TR?= NWn4eIlYW0GQR1XS
NB69 M2C1R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7KeJVKSzVyPUGuNFI6PDVizszN M{TsT3NCVkeHUh?=
YKG-1 M361b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTFwMEW0NVkh|ryP M4nNVnNCVkeHUh?=
EW-3 Mn;KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7qTWM2OD1zLkC3PFg{KM7:TR?= NXTpcYY1W0GQR1XS
GCIY M2jDU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7GR3EzUUN3ME2xMlA5OTB2IN88US=> MkDFV2FPT0WU
SK-MES-1 NGeyXpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mof3TWM2OD1zLkGxOFg4KM7:TR?= M{THfXNCVkeHUh?=
PANC-08-13 NX\JOVVxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XEdmlEPTB;MT6xOlQyPiEQvF2= M4XBenNCVkeHUh?=
SK-NEP-1 M3XsPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTFwMUe1NVMh|ryP Mnz6V2FPT0WU
SW954 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvWTWM2OD1zLkKwNlE1KM7:TR?= NGnKOIdUSU6JRWK=
NCI-SNU-5 NHHIOXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TNR2lEPTB;MT6yN|kh|ryP NYewfY1kW0GQR1XS
SW48 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vZRmlEPTB;MT6yOVQ{OiEQvF2= MnTLV2FPT0WU
HT-3 NV\GVXo{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEGxe25KSzVyPUGuNlg4PTNizszN NFj4V4NUSU6JRWK=
ETK-1 Ml;zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoP0TWM2OD1zLkO3OFU3KM7:TR?= MYnTRW5ITVJ?
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NCI-H630 NH3mb|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLyVmZKSzVyPUGuOFU2QDJizszN MVvTRW5ITVJ?
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ME-180 NUXRT3lVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnv5TWM2OD1zLk[xNFk3KM7:TR?= NGXzeWpUSU6JRWK=
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G-401 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HYe2lEPTB;MT62O|QyOyEQvF2= NHfzSHZUSU6JRWK=
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Detroit562 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHKTWM2OD1zLkewNVUzKM7:TR?= NFmwdlBUSU6JRWK=
OE19 NFfUUIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfrdmJWUUN3ME2xMlcyPTF{IN88US=> M3PHdHNCVkeHUh?=
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TE-12 NHW4R4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULkWYZ[UUN3ME2xMlgxPDF2IN88US=> MkPMV2FPT0WU
NCI-H1666 NULxPHJ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DPZWlEPTB;MT64NFk1QCEQvF2= Ml:0V2FPT0WU
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CAL-12T MmrkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPpTWM2OD1zLki4PVQ2KM7:TR?= NVvpZXd2W0GQR1XS
TE-8 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml6xTWM2OD1zLkmxN|c4KM7:TR?= MnflV2FPT0WU
KYSE-270 M4HF[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnqSm5wUUN3ME2yMlAzPzN{IN88US=> NYGxW|lpW0GQR1XS
GMS-10 Mk\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXTbm5nUUN3ME2yMlA{PTF5IN88US=> MYfTRW5ITVJ?
A2780 M4\5Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XBOmlEPTB;Mj6wOlI6PCEQvF2= NHm3NJFUSU6JRWK=
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NCI-H1048 MnTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7VTWM2OD1{LkS5NVU5KM7:TR?= Mn35V2FPT0WU
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TE-10 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUf1O|g{UUN3ME2yMlY5ODd6IN88US=> M3X4R3NCVkeHUh?=
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LB1047-RCC MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTJwN{S4PFgh|ryP NGHIS2ZUSU6JRWK=
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HT-29 M2nXcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmT1TWM2OD1|LkC3N|A6KM7:TR?= NXTZXllTW0GQR1XS
RPMI-7951 MlTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnrTWM2OD1|LkC3OVkh|ryP NUjSWnk6W0GQR1XS
NBsusSR NV;Be|ZST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jSVWlEPTB;Mz6xPFI2PCEQvF2= MlPhV2FPT0WU
NKM-1 NHLXc3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLDNnZmUUN3ME2zMlE5PzR2IN88US=> NHLw[phUSU6JRWK=
Capan-2 NWLjdFR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTNwNE[4O|Uh|ryP NIHRSm5USU6JRWK=
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OAW-42 Mo\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTNwNUCwNlYh|ryP MXzTRW5ITVJ?
SKG-IIIa MmCxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTaTWM2OD1|LkWyNlM4KM7:TR?= Ml;lV2FPT0WU
no-11 NYrNUmFsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnVc|ZRUUN3ME2zMlU{PTZ2IN88US=> NYPmRpV[W0GQR1XS
NY NYG5R|VMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3TV4t4UUN3ME2zMlU{PjV3IN88US=> NWnj[owzW0GQR1XS
HEC-1 NU\0XI5OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7odolKSzVyPUOuOVg6PSEQvF2= MoHiV2FPT0WU
NCI-H441 MkTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HyN2lEPTB;Mz61PVg1OSEQvF2= M4XXW3NCVkeHUh?=
NCI-H2009 NY[4XZZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHG1[lJKSzVyPUOuOlEzOTZizszN NVHmdIdUW0GQR1XS
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NCI-SNU-1 M17RTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXz3[|B6UUN3ME2zMlY6PzN3IN88US=> NFrUSJVUSU6JRWK=
BT-20 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4K0d2lEPTB;Mz62PVc6PSEQvF2= Ml\SV2FPT0WU
LS-123 M{jCeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfuNG1KSzVyPUOuO|QzQDRizszN MV;TRW5ITVJ?
8305C Mo\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fOZWlEPTB;Mz63OVkyOSEQvF2= NFLrNnlUSU6JRWK=
OCUB-M MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3QSWNuUUN3ME2zMlc3OTh5IN88US=> MmHRV2FPT0WU
KOSC-2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TaUWlEPTB;Mz64N|Q2OSEQvF2= MV;TRW5ITVJ?
NCI-H1693 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL6WHRRUUN3ME2zMlg1OThzIN88US=> MV7TRW5ITVJ?
TE-9 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTNwOESzPFIh|ryP MWLTRW5ITVJ?
MV-4-11 NF\2V5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4Lve2lEPTB;Mz64O|k6PCEQvF2= NHPObJlUSU6JRWK=
GAMG MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjRWHhLUUN3ME2zMlkyOzF2IN88US=> MXTTRW5ITVJ?
KNS-62 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vJVGlEPTB;Mz65OFc{OSEQvF2= MlXmV2FPT0WU
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U-87-MG NGi1bo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\ReGlEPTB;ND6xNFQ5OSEQvF2= NWDLWFJ{W0GQR1XS
RPMI-8226 NUDBcXBJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkm5TWM2OD12LkGxOlMyKM7:TR?= NITwVnVUSU6JRWK=
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HT-1197 NYjCc|FnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPFZ2dKSzVyPUSuOVI3PjlizszN NVK0TJh6W0GQR1XS
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HL-60 M3LQSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTRwOE[2NVEh|ryP M3j6[3NCVkeHUh?=
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MEG-01 M{TP[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoS1TWM2OD12Lkm5OFA{KM7:TR?= NGq5fIdUSU6JRWK=
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HC-1 M{P5PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vFfWlEPTB;NT61NVg{QSEQvF2= NGPTSmJUSU6JRWK=
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KINGS-1 Mn7MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1f3WWlEPTB;NT62NFk1PCEQvF2= Mnz1V2FPT0WU
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NCI-H2291 MoPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTVwOUi5OFMh|ryP NIHtV2xUSU6JRWK=
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SW756 MlrOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;ScGt6UUN3ME22MlkyOTd4IN88US=> NH\DcJpUSU6JRWK=
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NCI-H28 M1fBNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzlTWM2OD15LkG2OFA2KM7:TR?= M1T4[XNCVkeHUh?=
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COLO-800 MmK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTdwNEG0OFYh|ryP M4nDbXNCVkeHUh?=
DB NXzvcHNGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrz[VU1UUN3ME23MlYyODB3IN88US=> MnPxV2FPT0WU
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NH-12 M1PEO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PYfmlEPTB;Nz63NlgxQCEQvF2= M2G4OXNCVkeHUh?=
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GT3TKB MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\RNmNDUUN3ME24MlY4QTZ2IN88US=> MUTTRW5ITVJ?
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BALL-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3f6VmlEPTB;OT6yNVkxPyEQvF2= NV7VNJBYW0GQR1XS
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HuCCT1 NHviTFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NESxdVlKSzVyPUGwMlYxOzNizszN MVPTRW5ITVJ?
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CAL-72 M3v1c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1L2cmlEPTB;MUCuPVM6OSEQvF2= M4\qSnNCVkeHUh?=
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MC-IXC NYPFcYVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXW1[5VpUUN3ME2xNU43PzJ6IN88US=> MljWV2FPT0WU
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NOS-1 M2\Qbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXVcXY1UUN3ME2xNU44OTB7IN88US=> NFXk[mZUSU6JRWK=
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NCI-H596 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfqTWM2OD1zMj6wN|Y6KM7:TR?= Mon2V2FPT0WU
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HCC1937 NHLsXnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrv[mM5UUN3ME2xNk44PTZ2IN88US=> MlvNV2FPT0WU
SW837 M2Xqe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX[xZ3JQUUN3ME2xNk46ODJ6IN88US=> MlnHV2FPT0WU
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NCI-H2030 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXe1cZJYUUN3ME2xOE43PDR6IN88US=> M{HmdnNCVkeHUh?=
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EM-2 Ml20S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TPWGlEPTB;MUSuPFU4PiEQvF2= NVPWV4NSW0GQR1XS
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A2058 M{\hRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkSzTWM2OD1zNT6wOFA6KM7:TR?= MlOwV2FPT0WU
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MFE-280 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPiVGRKSzVyPUG2MlA1QDhizszN M2XoTnNCVkeHUh?=
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HH M2flO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPMTWM2OD1zNj61NVYzKM7:TR?= NGfycHRUSU6JRWK=
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NCI-H2405 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrNTWM2OD1zNz6xPVc1KM7:TR?= MWTTRW5ITVJ?
U-2-OS M3ryZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfldYJYUUN3ME2xO{42OzB5IN88US=> Mlr5V2FPT0WU
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MOLT-16 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHEXVFKSzVyPUG3Mlg6OzRizszN NGSwdHpUSU6JRWK=
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D-283MED M2j4XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYS3WWduUUN3ME2xPE4xODh6IN88US=> Ml\PV2FPT0WU
RCC10RGB NGizbWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTEdm9WUUN3ME2xPE4xPTV7IN88US=> NWO1NXhsW0GQR1XS
NCI-H1437 NWriUnFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHUflZSUUN3ME2xPE4yPjRzIN88US=> NGTiUGtUSU6JRWK=
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A704 M1L2Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33FVGlEPTB;MUiuOFc2OyEQvF2= M4C4dXNCVkeHUh?=
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SCH MmjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTF6Lk[0OVYh|ryP Mn3HV2FPT0WU
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Mewo MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HJU2lEPTB;M{SuNVM1QSEQvF2= MV;TRW5ITVJ?
22RV1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTQNGpzUUN3ME2zOE4yPzV2IN88US=> MW\TRW5ITVJ?
KM-H2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXjWI9KSzVyPUO0MlE4PzlizszN NUfFVJkyW0GQR1XS
NCI-H2122 NY\PcGoxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\pSXZNUUN3ME2zOE4{PDdzIN88US=> NXrWXY1DW0GQR1XS
ABC-1 NVTrNGlkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXr0VXZzUUN3ME2zOE4{Pjl|IN88US=> NHe5R5RUSU6JRWK=
NCI-H1355 MlHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXVTWM2OD1|ND6zPFg6KM7:TR?= Ml;iV2FPT0WU
NOMO-1 M3LjXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnu5TWM2OD1|ND61O|A{KM7:TR?= NUHie2d5W0GQR1XS
MZ7-mel NWL6bpN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUm2dmZbUUN3ME2zOE43PDh5IN88US=> MYPTRW5ITVJ?
NCI-H810 NVvPdphXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTN2LkeyO|gh|ryP MlKzV2FPT0WU
D-392MG M1HW[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTN3LkC1OlUh|ryP Mkn2V2FPT0WU
LB771-HNC MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rV[mlEPTB;M{WuNVY2QCEQvF2= MmXtV2FPT0WU
COLO-679 NIraOGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvZXI5KSzVyPUO1MlM5QCEQvF2= NHzlV4xUSU6JRWK=
SK-PN-DW MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nWb2lEPTB;M{WuOFM3QSEQvF2= M3\yXnNCVkeHUh?=
VA-ES-BJ M3KxUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\GeWlEPTB;M{WuOFY1QSEQvF2= M4TlOnNCVkeHUh?=
KNS-42 NUizcJFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{j5dGlEPTB;M{WuOVgzPiEQvF2= NYjn[HJ5W0GQR1XS
RVH-421 NUDTRlQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;6TWM2OD1|NT63OlQ2KM7:TR?= M3fSfnNCVkeHUh?=
P30-OHK MnPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTN3Lki3OVIh|ryP M4ToPXNCVkeHUh?=
G-402 MlfYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUf0blI5UUN3ME2zOU46QDZ5IN88US=> M1LUSnNCVkeHUh?=
S-117 MoLaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTN4LkOwNFgh|ryP Moq3V2FPT0WU
RXF393 NF64WJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfKTWM2OD1|Nj6zO|g2KM7:TR?= MnPLV2FPT0WU
LCLC-103H MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTN4Lke4NFMh|ryP M{WwdXNCVkeHUh?=
U-118-MG M4THfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjCS4hKSzVyPUO2Mlg5PDdizszN NUHsbVlNW0GQR1XS
8-MG-BA M{Tj[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTN4Lkm0PFMh|ryP M2KxU3NCVkeHUh?=
MFH-ino MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTN5LkOzO|Eh|ryP MnHRV2FPT0WU
NCI-H23 NGjuUXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3pTWM2OD1|Nz60NVk3KM7:TR?= MUfTRW5ITVJ?
NCI-H446 M2\WeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfJVJJKSzVyPUO3MlQ5PjJizszN MojTV2FPT0WU
LU-99A M1\vbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3oOYFKSzVyPUO3MlU4OzFizszN NEGyeHVUSU6JRWK=
KP-N-YN M3fZZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEG5d4tKSzVyPUO3MlYyQTVizszN NWDLZ29uW0GQR1XS
SCC-9 M3SzXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT2SGtXUUN3ME2zO{44QDN6IN88US=> Mn7qV2FPT0WU
EFM-19 MnfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTPTWM2OD1|Nz63PVY4KM7:TR?= NXS4Xpd1W0GQR1XS
A427 MofkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIW0VmlKSzVyPUO3MlkyODdizszN NEn6dpNUSU6JRWK=
LB831-BLC M1SwUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIi0OIFKSzVyPUO4MlYxOzlizszN M2L0fnNCVkeHUh?=
HOS NGTKXItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHz4PJNKSzVyPUO5MlYzPzNizszN NGqybI5USU6JRWK=
LAN-6 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XhTGlEPTB;NECuNFEyOiEQvF2= MUXTRW5ITVJ?
U251 NWTXNVZ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnDZ2hjUUN3ME20NE41ODV2IN88US=> M3\oTnNCVkeHUh?=
SNU-387 MoTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fBV2lEPTB;NECuOFU2OyEQvF2= MVnTRW5ITVJ?
DMS-79 NU\CPXNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3GcpBKSzVyPUSwMlU5QDlizszN MYnTRW5ITVJ?
SK-LU-1 NYHVblZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPuTWM2OD12MD62OVY5KM7:TR?= NV\mboxKW0GQR1XS
HT MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETNSmRKSzVyPUSwMlY3OzlizszN MnfiV2FPT0WU
KP-N-YS MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTRyLk[3Olgh|ryP NHTsVVRUSU6JRWK=
DMS-114 M1vkT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\2NXU5UUN3ME20NE45Ozd2IN88US=> MWXTRW5ITVJ?
HUTU-80 MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ryVWlEPTB;NEGuNFU3QSEQvF2= NYDRZpl7W0GQR1XS
ONS-76 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlu0TWM2OD12MT60O|A6KM7:TR?= NW\UfZFJW0GQR1XS
A673 MkjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULOe|VWUUN3ME20NU42OTV5IN88US=> MUPTRW5ITVJ?
HPAF-II NIjnbmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHJRW9lUUN3ME20NU43PzB7IN88US=> NWjZWI5pW0GQR1XS
UACC-257 MmPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLETWM2OD12MT63N|Y6KM7:TR?= M4DBfHNCVkeHUh?=
EW-22 MlzZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnWzTWM2OD12MT64Olk4KM7:TR?= M3O3NnNCVkeHUh?=
NCI-H1755 M1\M[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ri[mlEPTB;NEGuPFczPSEQvF2= M4nGU3NCVkeHUh?=
KYSE-150 M2mxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXITWM2OD12Mj6xO|gzKM7:TR?= NGXq[WVUSU6JRWK=
CGTH-W-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTR{LkS5OVUh|ryP MmfuV2FPT0WU
NB13 MoLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzLdndQUUN3ME20Nk43OjF5IN88US=> M4LTNHNCVkeHUh?=
SW872 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonuTWM2OD12Mj62N|MyKM7:TR?= M1X2b3NCVkeHUh?=
KP-4 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW5ZpN2UUN3ME20Nk45QDN{IN88US=> NFGzZWNUSU6JRWK=
HCC38 NXH4cpVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTR{Lkm2NVgh|ryP MoTGV2FPT0WU
BB49-HNC NYL6VG5bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jvVWlEPTB;NEOuNlM2PiEQvF2= NFjVZlBUSU6JRWK=
CAL-62 NETjSJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF[0VYNKSzVyPUSzMlQxOjhizszN MX\TRW5ITVJ?
NCI-H2029 NFzYXWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzvTWM2OD12Mz61OVgyKM7:TR?= NEHJZlRUSU6JRWK=
M14 NILIU5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTR2LkGzN|Ih|ryP NFrVSFdUSU6JRWK=
COLO-680N NV7zO5B6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIL2eGNKSzVyPUS0MlM{QSEQvF2= MnfaV2FPT0WU
Hs-578-T NGrXfXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3nTWM2OD12NT6wNlk1KM7:TR?= M3PxdnNCVkeHUh?=
MSTO-211H NVfofGlpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfTSnBKSzVyPUS1Mlk1QDJizszN NGLHbnhUSU6JRWK=
EW-1 NEiwdmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjpPZNKSzVyPUS1Mlk3QDlizszN MoDhV2FPT0WU
SW948 NHHNOZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTR4LkS0OlIh|ryP NHrocmdUSU6JRWK=
NCI-H460 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vIUmlEPTB;NE[uOVI{KM7:TR?= MXzTRW5ITVJ?
CAS-1 Mk\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTR4Lke3NFEh|ryP NFn2ZWpUSU6JRWK=
TGBC11TKB MnLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTR5LkG3O|ch|ryP NVfnfIxZW0GQR1XS
HD-MY-Z NELGOJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\5T|hKSzVyPUS4Mlc5PiEQvF2= NYja[VI{W0GQR1XS
NCI-H226 NWfLSolpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3qTWM2OD12OT63O|g2KM7:TR?= MlnqV2FPT0WU
PA-1 NVTSPVd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTR7LkizN|Uh|ryP MnLDV2FPT0WU
LB2518-MEL M3LjXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTR7Lki3NlMh|ryP NFrhV2NUSU6JRWK=
A101D Ml;ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILuWIdKSzVyPUS5Mlk1OjVizszN M1nLe3NCVkeHUh?=
NCI-H2087 MlrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVS1W|NTUUN3ME20PU46Pjh{IN88US=> NHHuT3BUSU6JRWK=

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

+ 展開

In vitro kinase activity assays:

The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.
細胞試験:

[1]

+ 展開
  • 細胞株: NSCLC cells
  • 濃度: 0-10 μM
  • 反応時間: 1 hour
  • 実験の流れ:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.


    (参考用のみ)
動物試験:

[1]

+ 展開
  • 動物モデル: Athymic NMRI-nu/nu female mice
  • 製剤: In 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • 投薬量: 20 mg/kg
  • 投与方法: Oral administration
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water slightly soluble or insoluble
体内 順序で溶剤を入れること:
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
10mg/mL

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 485.94
化学式

C24H25ClFN5O3

CAS No. 439081-18-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02795156 Recruiting Non-small Cell Lung Carcinoma|Urothelial Carcinoma|Gastrointestinal Carcinoma, Non-colon|Upper Aerodigestive Tract Carcinoma SCRI Development Innovations, LLC|Foundation Medicine|Boehringer Ingelheim|Bayer September 28, 2016 Phase 2
NCT03054038 Not yet recruiting Non-Small Cell Lung Carcinoma Vanderbilt-Ingram Cancer Center|National Cancer Institute (NCI) February 2017 Phase 1
NCT02979977 Not yet recruiting Squamous Cell Cancers of the Head and Neck Aarti Bhatia|National Comprehensive Cancer Network|Boehringer Ingelheim|Yale University December 2016 Phase 2
NCT02876081 Not yet recruiting SMALL CELL LUNG CARCINOMA Groupe Hospitalier Paris Saint Joseph December 2016 Phase 2
NCT02183883 Recruiting Non-small Cell Lung Cancer University College, London|Boehringer Ingelheim December 2016 Phase 2
NCT02423525 Recruiting Brain Cancer Santosh Kesari|Boehringer Ingelheim|John Wayne Cancer Institute October 2016 Phase 1

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • 回答:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

EGFR信号経路図

EGFR Inhibitors with Unique Features

相関EGFR製品

Tags: Afatinib (BIBW2992)を買う | Afatinib (BIBW2992) ic50 | Afatinib (BIBW2992)供給者 | Afatinib (BIBW2992)を購入する | Afatinib (BIBW2992)費用 | Afatinib (BIBW2992)生産者 | オーダーAfatinib (BIBW2992) | Afatinib (BIBW2992)化学構造 | Afatinib (BIBW2992)分子量 | Afatinib (BIBW2992)代理店
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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID