Gefitinib (ZD1839)

製品コードS1025

Gefitinib (ZD1839)化学構造

分子量(MW):446.90

Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。

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カスタマーフィードバック(12)

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。
特性 A potent EGFR tyrosine kinase inhibitor.
ターゲット
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外試験

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGG5OGdKSzVyPUCuNFU1KM7:TR?= M1O5VFE3PTF4NEez
NIH3T3 NVL4N4E6TnWwY4Tpc44hSXO|YYm= MnPmNE4zKM7:TR?= Mn7QNkBp MkLNSG1UVw>? M{HyOWlvcGmkaYTpc44hd2ZiRVfGJJN1cW23bHH0[YQhcHWvYX6g[ZJjSjFiYYX0c5Bpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUS0JO69VQ>? NGW1VnIyPjR6MEK4OC=>
COS7 MoHqSpVv[3Srb36gRZN{[Xl? MVO5OkDPxE1? NXTD[HFwUW6qaXLpeIlwdiCxZjDoeY1idiCMQVuyJHY3OTeIIH31eIFvfCCneIDy[ZN{\WR? Ml\VNVcyPzh5MkK=
A549 MXHDfZRwfG:6aXOgRZN{[Xl? NITNR5M6PiCq NEn5WXlFVVOR MmH4TWM2OD1zMz61PUDPxE1? M{e5cFE5OzF|OEC3
K562 NGrvU3FEgXSxdH;4bYMhSXO|YYm= NXfNeFE4QTZiaB?= NUiyS2FmTE2VTx?= NWW2OFIyUUN3ME25MlM3KM7:TR?= MVKxPFMyOzhyNx?=
MDA-MB-231 NIe4VHJEgXSxdH;4bYMhSXO|YYm= M4jQN|k3KGh? MnvZSG1UVw>? M13NUGlEPTB;M{[uOlEh|ryP MVmxPFMyOzhyNx?=
MOLT4 NILTfJNEgXSxdH;4bYMhSXO|YYm= MWO5OkBp NYLlOJJiTE2VTx?= M4e5XmlEPTB;MUWuNFIh|ryP M1;mR|E5OzF|OEC3
MCF7 M2XiRWN6fG:2b4jpZ{BCe3OjeR?= NFL6V2E2OCEQvF2= NFrnc2I4OiCq MXLJR|UxRTF{LkC1JO69VQ>? M{H3dlE5PzdzOEG5
BT474 MlfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYq3NkBp NWXueplmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFQ4PCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFVmJjOiC5aYToJGVEPTBib3[gNE4{KM7:TR?= NFO4WGwyQTB{OESyOC=>
HN5 M1jUOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWK3NkBp MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiQNTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghTUN3MDDv[kAxNjBzIN88US=> MXyxPVAzQDR{NB?=
BA/F3 NIPLSVNEgXSxdH;4bYMhSXO|YYm= M2LMelczKGh? MnvZR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> M1LTdVE6OjN7MkK5
NIH3T3 M4PPZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PIZlczKGh? M3O2UGdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUC9|VEOgZ4VtdHNiZYjwdoV{e2mwZzDldmIzKGenbnWge4l1cCCLQ{WwJI9nKDBwNUSg{txO MX:xPVY3PTN5Nx?=
MCF7 MX NIXUR5pHfW6ldHnvckBCe3OjeR?= MWezNEBucW5? MkP6TY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EB4cXSqIFnDOVAhd2ZiMT6xJO69VQ>? M4LqdFE6QTN{OU[w
A431 MlH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;ZNVAh|ryP MlvTO|IhcA>? NUfqT3BPT0l3ME2wMlUyPCEQvF2= MljlNVk6Pjl2NkW=
A431 MlLJT4lv[XOnIFHzd4F6 MYiwMlEh|ryP NVG3WJV5OSCq NH3wUItKdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v MonNNVk6Pjl2NkW=
A431 NEm5cJlMcW6jc3WgRZN{[Xl? M3;rXFExOCEQvF2= MoDlNVAhdWmw M2nROnBzd2yxbnfl[EBqdmirYnn0c5J6KGGldHn2bZR6KG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v MmG5NVk6Pjl2NkW=
Sf9 NGPmclVMcW6jc3WgRZN{[Xl? NIK0UJgyOCCvaX6= NXzXcoY4TE2VTx?= M2\Pc2lvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= MYWyNFA2PjR{NR?=
NCI-H1975 MnTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnXdphFPSEQvF2= Mn73O|IhcA>? MWfJR|UxRThwMk[g{txO NX7wWWxjOjBzNUG2O|A>
NCI-H1975 MYHLbY5ie2ViQYPzZZk> NVnmPHVxOTBizszN MULJcohq[mm2aX;uJI9nKEWJRmKgZZV1d3Cqb4PwbI9zgWyjdHnvci=> Mo\QNlAyPTF4N{C=
NCI-H1975 M3y3d2Z2dmO2aX;uJGF{e2G7 MofHNVAh|ryP MVjJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> M4TCUlIxOTVzNkew
NCI-H1975 M4rse2Z2dmO2aX;uJGF{e2G7 NInrV3Y2KM7:TR?= NIDKXm01QCCq NH7USJdKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiY3HzdIF{\S1| MmfONlAyPTF4N{C=
A375 NVPoeFEyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;CRWk1QCCq M{L3WWlEPTB;MkCuOVUh|ryP NUHyeXdEOjB|MES1N|c>
SMMC7721 NWrhSnp1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUC0PEBp NYDYOmdwUUN3ME2zN{4yOiEQvF2= MnfoNlA{ODR3M{e=
DU145 NGLyfmREgXSxdH;4bYMhSXO|YYm= M{LGOlUh\A>? NUXyWWcxTE2VTx?= NYXrS2ZvUUN3ME20Nk4yPyEQvF2= MUGyNFQ3PjV3NR?=
MIAPaCa2 NF25TI1EgXSxdH;4bYMhSXO|YYm= NEXXUXE2KGR? MlW0SG1UVw>? MWrJR|UxRTVyLk[yJO69VQ>? M2G4VFIxPDZ4NUW1
PANC1 M332eWN6fG:2b4jpZ{BCe3OjeR?= MYm1JIQ> Mn;MSG1UVw>? M3WzVmlEPTB;NECuOFIh|ryP MoXwNlA1PjZ3NUW=
PC9 NXnlZ5NGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfFNmlWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGT0[ULXTl[olkcWWwdDDoeY1idiCSQ{mgZ4VtdHNid3n0bEBGSzVyIH;mJFAvODF5IN88US=> MlXaNlEzODh6MEK=
PC9 NEfQWmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWi1NZAzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> NEnqWYIzOTJyOEiwNi=>
PC3 NE\lNI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfvZZVpOTBizszN NFfO[|E4OiCq NUTsXXFFTE2VTx?= MWPJR|UxRTdwNDFOwG0> NWq3b5dDOjF|NUO1OFY>
MDA-MB-468 M3PDWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofENVAxKM7:TR?= NX7VOZhtPDhiaB?= M{jQUWROW09? M{i5dGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 NWL2eVlTOjF2Mkm2N|I>
Plasmodium falciparum M332cpFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w MlHFNlE5OTdyNEW=
NCI-H1993 NFOyPIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M123eVczKGh? NVjYPHF[TE2VTx?= M3u5VmlEPTB;MD6xJO69VQ>? M13JV|IyQTF4NEOz
NCI-H292 M{HU[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXi3NkBp MXfEUXNQ M1HtNWlEPTB;MD6wNkDPxE1? M2rSeFIyQTF4NEOz
A431 NXzESFVJU2mwYYPlJGF{e2G7 NV7UOmVsOiCq NWTlPJR4TE2VTx?= NFTDV4lKdmirYnn0bY9vKG:oIFXHSnIheGixc4Doc5J6dGG2aX;uJIlvKEWJRj3zeIlufWyjdHXkJIh2dWGwIFG0N|Ehf2m2aDDJR|UxKG:oIECuNFAyKM7:TR?= MYeyNlExOTF|Mh?=
MCF7 MnXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH2ycnoyODEEoN88[{9uVA>? NXHqfVZ2PDhiaB?= M4XRdGROW09? MnXsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN NX64eVZsOjJzMUmxN|A>
BT474 Ml[xR5l1d3SxeHnjJGF{e2G7 M{jnVlExKM7:TR?= MVO0PEBp MnrLR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= NUHTdFF{OjJzM{izNFg>
MCF7 NYH0WWoxS3m2b4TvfIlkKEG|c3H5 NYHYVpNuOTBizszN MWG0PEBp MYTDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKE2FRkegZ4VtdHNib4\ldk1mgHC{ZYPzbY5oKEW{YlKyJINmdGy| MVSyNlE{QDNyOB?=
HCC827 M3vwdWtqdmG|ZTDBd5NigQ>? NHXB[3oxNjF{NTFOwG0> NELtTVIzPCCq MX;EUXNQ NFTCTW5KdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? NHLSUWgzOjN|OUO0Ni=>
HCC827 NIPhXmNMcW6jc3WgRZN{[Xl? NFjKUWwxNjF{NTFOwG0> NUjRRldpOjRiaB?= NV7DW5lrTE2VTx?= M{PFU2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCHcnugdIhwe3Cqb4L5cIF1cW:w NEHM[XMzOjN|OUO0Ni=>
HCC827 M3OxWmtqdmG|ZTDBd5NigQ>? M{m5[FAvOTJ3IN88US=> NEP4OngzPCCq MYjEUXNQ MVrJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhSWu2IIDoc5NxcG:{eXzheIlwdg>? MlnjNlI{Ozl|NEK=
A431 M3H3emtqdmG|ZTDBd5NigQ>? M3HkOVExOCEQvF2= NFHZSWE{OCCvaX6= NFy0eoZKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN M2H3[FIzPjl6N{iy
H460 MlqzR5l1d3SxeHnjJGF{e2G7 MWK3NkBp MnnCTWM2OD13LkW5JO69VQ>? Mn3rNlI4Ojd2NEm=
HT-29 NFTX[VVEgXSxdH;4bYMhSXO|YYm= NHP4R|c4OiCq NXz1coE6UUN3ME2zMlM3KM7:TR?= MY[yNlczPzR2OR?=
SGC7901 M4\zXWN6fG:2b4jpZ{BCe3OjeR?= NF;HcGQ4OiCq NGrrZlhKSzVyPUGwMlI3KM7:TR?= MWeyNlczPzR2OR?=
A498 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjyd|ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= NF63SGYzOjhzOEi0PC=>
Calu3 NYTpU4dIS3m2b4TvfIlkKEG|c3H5 MkH2NVAh|rypL33M NYPCU|d[PzJiaB?= M{\GdWlEPTB;MT62JO69VQ>? NW\MWXZrOjNzMU[xOlg>
FADU NWTuWVJ5S3m2b4TvfIlkKEG|c3H5 NHTsN4EyOCEQvHevcWw> MmL6O|IhcA>? NVH5blVHUUN3ME2wMlA2KM7:TR?= MojWNlMyOTZzNki=
HCC827 MoXBR5l1d3SxeHnjJGF{e2G7 M1LZNVExKM7:Zz;tUC=> MmTjO|IhcA>? MX7JR|UxRTBwMECxOUDPxE1? MUKyN|EyPjF4OB?=
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IGROV-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTJwM{GyN|ch|ryP M{DnVnNCVkeHUh?=
JVM-3 MmLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPoTWM2OD1{LkO0OlQ{KM7:TR?= MVfTRW5ITVJ?
NCI-H2170 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFG2V5lKSzVyPUKuN|Q4QDNizszN MVzTRW5ITVJ?
EW-18 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\MWo9EUUN3ME2yMlM2PDdzIN88US=> NYK2WmkyW0GQR1XS
KU812 NYrMTW9bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nC[GlEPTB;Mj6zOlc4QSEQvF2= NF3PdpFUSU6JRWK=
NY NE[0b4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXnZmZ2UUN3ME2yMlM4OjdizszN MonPV2FPT0WU
HTC-C3 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTJwNEKyOlch|ryP NX22VJd2W0GQR1XS
CFPAC-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTJwNEO4OlQh|ryP NXfzU2pVW0GQR1XS
HH MknIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;i[Y9KSzVyPUKuOFk5PTRizszN M{nUNXNCVkeHUh?=
MKN7 M2DNNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILZfIlKSzVyPUKuOlI4PDhizszN M1;m[XNCVkeHUh?=
TE-8 NFnLdZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTJwNkO2OVMh|ryP NGS4doNUSU6JRWK=
MC-IXC MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;0VYtKSzVyPUKuOlQ1PTNizszN M2TnfHNCVkeHUh?=
DMS-79 NIftd2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTJwNkmyPFQh|ryP MkjyV2FPT0WU
Detroit562 NIrvdXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfkZW9KSzVyPUKuOlk5PDVizszN MXLTRW5ITVJ?
HuP-T4 MnvDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rXO2lEPTB;Mj63NlgyQCEQvF2= NFjSUJhUSU6JRWK=
QIMR-WIL MlTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVL6NotIUUN3ME2yMlc2QTV{IN88US=> M33CNHNCVkeHUh?=
BB65-RCC Moi1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJwOEC3NVMh|ryP MXjTRW5ITVJ?
PC-3 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TRSGlEPTB;Mj64N|MyPyEQvF2= M1PzVXNCVkeHUh?=
OVCAR-3 Mlv4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTJwOEW1PVQh|ryP NVHjUFdWW0GQR1XS
KYSE-510 NGfZPI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;6VWlEPTB;Mj64Olc5PiEQvF2= NEXxdndUSU6JRWK=
HC-1 NV3odHVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnPTWM2OD1{Lkm0NFEzKM7:TR?= MmnQV2FPT0WU
NCI-H1693 NVLKUoNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfBVJY{UUN3ME2yMlk2QDl5IN88US=> NGDs[opUSU6JRWK=
DU-4475 M1zpNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrRTWdKSzVyPUKuPVkxPTJizszN MnO4V2FPT0WU
COLO-680N MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnYTWM2OD1|LkCwOFk2KM7:TR?= NVGxRnB2W0GQR1XS
MN-60 Mn6zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDrTWM2OD1|LkC0PVc1KM7:TR?= MV3TRW5ITVJ?
BCPAP M{LCbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE[2bVlKSzVyPUOuNFgzQTVizszN M4m0[nNCVkeHUh?=
647-V M4rDfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTNwMU[yNkDPxE1? MnnEV2FPT0WU
HT-1376 NH;UXZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrSbXNKSzVyPUOuNlA2ODZizszN NUDTOGZ3W0GQR1XS
NCI-H1793 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPuXVduUUN3ME2zMlIyODl6IN88US=> MkPMV2FPT0WU
SW1463 M3yzdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4H0WGlEPTB;Mz6yOlE6OiEQvF2= MW\TRW5ITVJ?
NCI-H727 NGL0RWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PmdGlEPTB;Mz6zPFU5PCEQvF2= NVH0W4lVW0GQR1XS
LB771-HNC Mm\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIiwPVNKSzVyPUOuN|kyOjZizszN M1\EenNCVkeHUh?=
D-542MG Mm\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlu4TWM2OD1|LkS0NlIzKM7:TR?= M1S0NHNCVkeHUh?=
NTERA-S-cl-D1 Mn\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v3NmlEPTB;Mz60OFk2PyEQvF2= M3LYdnNCVkeHUh?=
C8166 MmLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1WzOGlEPTB;Mz60OlUzOSEQvF2= NVvnXIxjW0GQR1XS
639-V NIPxTY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTNwNUe3PVIh|ryP Mlm2V2FPT0WU
TE-1 NGfjXlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInWXYVKSzVyPUOuOVkyPjlizszN NGrjO4lUSU6JRWK=
OAW-28 NFnKeFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjCWnN[UUN3ME2zMlU6PDJ4IN88US=> MVnTRW5ITVJ?
SK-LU-1 NWXqXVhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDGdWljUUN3ME2zMlYxPjR3IN88US=> M1nqfXNCVkeHUh?=
CaR-1 M2jINWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTNwNkGwOFgh|ryP MmGzV2FPT0WU
A2780 M2\WbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGq5fYlKSzVyPUOuOlQxQDlizszN M3Ww[XNCVkeHUh?=
C2BBe1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTNwNkS0NFch|ryP NFTxXFhUSU6JRWK=
SK-MES-1 MnLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{W4RmlEPTB;Mz62OFYyPCEQvF2= MYPTRW5ITVJ?
PANC-10-05 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTNwN{KwO|Ih|ryP NY[2[WRUW0GQR1XS
J-RT3-T3-5 NX7XT5lkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTNwN{W0NFYh|ryP M1HtNXNCVkeHUh?=
ES4 M1nu[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzUW5lKSzVyPUOuPFIzQDZizszN NXrOc2tTW0GQR1XS
NCI-H28 NULieGpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFn4V3ZKSzVyPUOuPFI6PjFizszN NHHSXphUSU6JRWK=
NCI-H1155 NXzjdYd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF30[JVKSzVyPUOuPFQyPTlizszN NVKzZ4s2W0GQR1XS
KURAMOCHI MlG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPE[mdJUUN3ME2zMlg3ODV4IN88US=> MnrPV2FPT0WU
NCI-H69 NVLZRnRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDRWpc{UUN3ME2zMlkxOTd6IN88US=> NWTZVYZiW0GQR1XS
IST-MES1 M4T0[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTMSYw2UUN3ME2zMlk1ODJ7IN88US=> M1:yXXNCVkeHUh?=
NH-12 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4T3PGlEPTB;Mz65OFE5PyEQvF2= M13R[HNCVkeHUh?=
JVM-2 M37Cd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTNwOUS3OVIh|ryP MX7TRW5ITVJ?
EW-16 Ml;pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnCSGoxUUN3ME2zMlk2PTd5IN88US=> Mmi3V2FPT0WU
NCI-H441 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTNwOUewNVQh|ryP NHjqdpdUSU6JRWK=
RCC10RGB NV;EUnE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moi3TWM2OD12LkCxNlQ3KM7:TR?= NFr5WJRUSU6JRWK=
TCCSUP MnjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTRwMUGwOUDPxE1? MUfTRW5ITVJ?
HL-60 NXXzW4NuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTRwMUKyOFEh|ryP NXmxS5M6W0GQR1XS
EW-11 MlXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjuO2NwUUN3ME20MlE{OjZzIN88US=> NIPCUmxUSU6JRWK=
KARPAS-299 NVvjWHdOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTHdFVKSzVyPUSuN|ExOTNizszN NEPENI5USU6JRWK=
SNU-C2B M3q3Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPveVdKSzVyPUSuN|Q3QDZizszN NXexRlh[W0GQR1XS
LU-139 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2D6TWlEPTB;ND6zO|I1PyEQvF2= NFrNN|JUSU6JRWK=
HCC1937 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rMW2lEPTB;ND6zO|k6OyEQvF2= NX\HfoN6W0GQR1XS
GR-ST MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnKPGZKSzVyPUSuOFMyQCEQvF2= NH23T2FUSU6JRWK=
SK-OV-3 Mnr5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnP5TWM2OD12LkS2NFQzKM7:TR?= NY\zPIx1W0GQR1XS
A172 NGjGUnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvCTWM2OD12LkW1OVIyKM7:TR?= MoD0V2FPT0WU
NB10 M2XXZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTRwNUm3PFEh|ryP MXLTRW5ITVJ?
P30-OHK NVPSOGdpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;YS2lEPTB;ND64NVkzQSEQvF2= MU\TRW5ITVJ?
KYSE-520 NFLaZ|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17USWlEPTB;ND64PFAyQCEQvF2= M2nDcXNCVkeHUh?=
KNS-62 NU\0SJlET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofETWM2OD12LkmwPFA4KM7:TR?= MWPTRW5ITVJ?
ES1 M{jkcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TVd2lEPTB;ND65OlA{PSEQvF2= MnPCV2FPT0WU
KOSC-2 NXzPPFkzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XHdmlEPTB;ND65PVk2QSEQvF2= M4LrPHNCVkeHUh?=
TE-9 NXexVlVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVT0ZmxsUUN3ME21MlAyPTJ3IN88US=> MUnTRW5ITVJ?
NCI-H1838 NH36S5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjIRWxvUUN3ME21MlAzODd4IN88US=> MYfTRW5ITVJ?
KP-N-YN NUXGVYFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTVwMUK1PVEh|ryP M3H4RXNCVkeHUh?=
ALL-PO M3\wfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\TO2lEPTB;NT6yNlU5PyEQvF2= MWXTRW5ITVJ?
NOMO-1 MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTVwMkOxO|Yh|ryP NF;3d|JUSU6JRWK=
CGTH-W-1 M4D0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT6RoRMUUN3ME21MlM5PTh2IN88US=> MVnTRW5ITVJ?
ATN-1 NGC1OlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jET2lEPTB;NT60NVE5PCEQvF2= MVfTRW5ITVJ?
AGS MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1[0R2lEPTB;NT60PVYyQCEQvF2= MXnTRW5ITVJ?
SW13 M2L4Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fyN2lEPTB;NT61N|I3PSEQvF2= MWXTRW5ITVJ?
SW1990 NF3STZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnwcFhKSzVyPUWuOlIzQDVizszN MUfTRW5ITVJ?
BFTC-905 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnDTWM2OD13Lk[1PFc5KM7:TR?= NWewNHFSW0GQR1XS
RMG-I M{P4NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3q0U2lEPTB;NT62OVk1OiEQvF2= NGCxT|lUSU6JRWK=
HD-MY-Z NEXze2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP6SFJtUUN3ME21MlY5PThzIN88US=> NY[y[pA4W0GQR1XS
HCC1187 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXy1[2NLUUN3ME21MlcyODN{IN88US=> NX;6Vmg{W0GQR1XS
HCC2998 NV\MWHgxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTVwN{G4O|ch|ryP MnrVV2FPT0WU
RT-112 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVW2[HY6UUN3ME21MlkxOjR3IN88US=> NXLmNYdCW0GQR1XS
CAKI-1 NGezW21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDoO3RKSzVyPU[uNFA6QDVizszN NIXlZpBUSU6JRWK=
HDLM-2 MmfUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO4cY5DUUN3ME22MlAyPDh7IN88US=> NVrEVJVXW0GQR1XS
TE-10 MlzYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fxPGlEPTB;Nj6wOFg{OyEQvF2= NVnue5QyW0GQR1XS
KE-37 NILvd29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoL0TWM2OD14LkC3NlU5KM7:TR?= MUXTRW5ITVJ?
RCM-1 M{jVcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV:1SINSUUN3ME22MlA4PzF7IN88US=> MmfoV2FPT0WU
Mewo NIXHdnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTZwMUG0PFch|ryP M4fjW3NCVkeHUh?=
L-428 M3\1SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTZwMUiwN|ch|ryP NVzQ[FY6W0GQR1XS
8-MG-BA M3qxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{W5XmlEPTB;Nj6xPVM4QSEQvF2= M{LLVXNCVkeHUh?=
SK-MEL-28 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvHSGJKSzVyPU[uNlA6PjdizszN MnXHV2FPT0WU
VA-ES-BJ NI\5V|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTZwM{C0OlIh|ryP MoW1V2FPT0WU
CAL-120 NXTJO4lST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTZwNEWxOlIh|ryP MWPTRW5ITVJ?
GAMG MnzoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:4TWM2OD14LkWwOlIh|ryP NVj3c2NtW0GQR1XS
MSTO-221H NXG2SIt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4ryTmlEPTB;Nj61NVE4KM7:TR?= NHnwTm9USU6JRWK=
GCT NFXJdo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmf4TWM2OD14LkWzN|g4KM7:TR?= MkXRV2FPT0WU
SH-4 MkG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;uS4FKSzVyPU[uOVg5PDRizszN M2PuW3NCVkeHUh?=
Calu-3 Mof6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLHTWM2OD14Lk[1OVY5KM7:TR?= M2r6b3NCVkeHUh?=
SK-HEP-1 NF3OeYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXq4NGRCUUN3ME22MlcyPzV|IN88US=> MkXWV2FPT0WU
PFSK-1 MlfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrHTHNKSzVyPU[uO|M1PzhizszN NIXnSJNUSU6JRWK=
NB12 NXPTUWJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTZwN{i0NFQh|ryP NFP2XVlUSU6JRWK=
A388 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTZwOEm1NkDPxE1? Ml;kV2FPT0WU
KLE NVvZbnp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrqTWM2OD14Lkm2OVE3KM7:TR?= MWHTRW5ITVJ?
TE-11 M2W3bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTSXXplUUN3ME23MlA1QTZ5IN88US=> MkSxV2FPT0WU
NCI-H2405 NUHBc5NoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInYWm5KSzVyPUeuNFg{QTNizszN NIfMendUSU6JRWK=
OVCAR-4 MoXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3H6TGlEPTB;Nz6xOVM2KM7:TR?= NGXpSFhUSU6JRWK=
KP-N-YS MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jq[GlEPTB;Nz6xPFE1KM7:TR?= M2H5OHNCVkeHUh?=
NOS-1 NWHxOWF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGW1UndKSzVyPUeuNlU5OjhizszN NUTVW4pmW0GQR1XS
DMS-114 M33ZPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITMOGJKSzVyPUeuNlk3OzFizszN M3HmdnNCVkeHUh?=
NCI-H2030 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33XcWlEPTB;Nz6zN|Y6QCEQvF2= MYDTRW5ITVJ?
HEL NVryTVFzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHzelZoUUN3ME23MlM4PTJzIN88US=> MlTyV2FPT0WU
CAL-62 NF;1WGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTdwM{m4OlYh|ryP NV33V5VsW0GQR1XS
COLO-668 NFvvbVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;MTWM2OD15LkSzPVY4KM7:TR?= MXjTRW5ITVJ?
SKG-IIIa NHnOcplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;Bb2VzUUN3ME23MlQ2OzhzIN88US=> M{\pT3NCVkeHUh?=
HCC1419 NGnZV3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;pbYtKSzVyPUeuOFY5OzNizszN MlLVV2FPT0WU
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NB14 NEHLdXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVf0NVFKUUN3ME2zPE41PTR2IN88US=> M1nYN3NCVkeHUh?=
D-423MG MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXCOnlKSzVyPUO4MlQ5PjlizszN NFvwWXZUSU6JRWK=
NCI-H510A NELEN4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDLPGRKSzVyPUO4MlY1PTFizszN NWnIZYFIW0GQR1XS
NMC-G1 MoL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHpTWM2OD1|OD63NlIzKM7:TR?= NUDGU4liW0GQR1XS
SW948 NYXaSZNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHkXHNKSzVyPUO4Mlg6PDlizszN NIDIeFdUSU6JRWK=
MFM-223 NUTN[HFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjJTWM2OD1|OD65OFMyKM7:TR?= NXrYcIJtW0GQR1XS
PAN1 M4fmfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nyXGlEPTB;M{iuPVkyQSEQvF2= MXzTRW5ITVJ?
MZ7-mel MkDGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LNNmlEPTB;M{muNVE6QSEQvF2= NWLSWo13W0GQR1XS
COLO-679 NIP2[GNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;1dHhKSzVyPUO5Mlk4ODhizszN M1\P[3NCVkeHUh?=
SW872 NUfBZ2QxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTRyLkKwN|Eh|ryP NGPVdHpUSU6JRWK=
HT-1197 NHnPR3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrvVlZKSzVyPUSwMlI5PjlizszN NXSyUXZjW0GQR1XS
ES6 Moi1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f4eGlEPTB;NECuOFU2OiEQvF2= MWXTRW5ITVJ?
SNU-387 NHTYZXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGT2NZZKSzVyPUSwMlgzOzhizszN M2[4WHNCVkeHUh?=
T47D MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTRzLkCxPVIh|ryP M2iwe3NCVkeHUh?=
G-402 M3zIOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEGzO4FKSzVyPUSxMlA5ODlizszN NFH3VlNUSU6JRWK=
MZ1-PC Ml6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGr5O4pKSzVyPUSxMlgyKM7:TR?= NHnqNFNUSU6JRWK=
SW1417 M2DiRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTR{LkOwN{DPxE1? NE\oZo1USU6JRWK=
RERF-LC-MS MoPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjLcld6UUN3ME20Nk42ODh5IN88US=> MYLTRW5ITVJ?
SF539 MoO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTR{LkW1Nlkh|ryP NH;TTmhUSU6JRWK=
ESS-1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mon3TWM2OD12Mz61OVU5KM7:TR?= M2HKTXNCVkeHUh?=
RO82-W-1 NHHGcHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnm1TWM2OD12Mz62NVU{KM7:TR?= NWLaUos6W0GQR1XS
S-117 M3v5VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjGTFF4UUN3ME20N{43QTV6IN88US=> MlrJV2FPT0WU
SW756 NUTHUlc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHX2[I1KSzVyPUSzMlgzPDJizszN NX33NGlKW0GQR1XS
CP66-MEL M4fsPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTR3LkexOFMh|ryP NXTid4wzW0GQR1XS
H-EMC-SS MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIeySYdKSzVyPUS1Mlc3QTNizszN MWfTRW5ITVJ?
NCI-H2052 NF7hZ4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LjO2lEPTB;NEWuPVAzPyEQvF2= NFfRU4tUSU6JRWK=
SW684 MnnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTR5LkCwPFEh|ryP NYTp[4FRW0GQR1XS
HCC1569 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTR5LkG2OVgh|ryP MWHTRW5ITVJ?
KGN M1zsRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPqTWM2OD12Nz6zN|EyKM7:TR?= M4KyXnNCVkeHUh?=
LCLC-103H NXLqVmhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PaT2lEPTB;NEeuOFQxPCEQvF2= MX;TRW5ITVJ?
SW780 MlvoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzhTWM2OD12Nz62N|UyKM7:TR?= NIPzZndUSU6JRWK=
NCI-H1703 M1fOR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\VT4hKSzVyPUS4MlM{PTJizszN MkTuV2FPT0WU
HCC1395 M{j6bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTR6Lke0N|Ih|ryP MYLTRW5ITVJ?

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

お薦めの試験操作(参考用のみ)

細胞試験: [1]
+ 展開
  • 細胞株: NR6, NR6M and NR6W cells
  • 濃度: 0-2 μM
  • 反応時間: 72 hours
  • 実験の流れ: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (参考用のみ)
動物試験:[3]
+ 展開
  • 動物モデル: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • 製剤: 0.5% polysorbate
  • 投薬量: 100 mg/kg
  • 投与方法: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water slightly soluble or insoluble
体内 順序で溶剤を入れること:
5% DMSO+corn oil
2.5mg/mL

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 446.90
化学式

C22H24ClFN4O3

CAS No. 184475-35-2
保管
in solvent
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

EGFR信号経路図

EGFR Inhibitors with Unique Features

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID