Gefitinib (ZD1839)

製品コードS1025

Gefitinib (ZD1839)化学構造

分子量(MW):446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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JPY 44820.00

文献中の使用例(92)

カスタマーフィードバック(13)

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

    Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

  • MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
特性 A potent EGFR tyrosine kinase inhibitor.
ターゲット
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外試験

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MlLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwMEW0JO69VQ>? NI\XPZEyPjVzNkS3Ny=>
NIH3T3 NGHvN4FHfW6ldHnvckBCe3OjeR?= NELPcVQxNjJizszN M4Pld|IhcA>? NXGzfpdyTE2VTx?= M3LhUWlvcGmkaYTpc44hd2ZiRVfGJJN1cW23bHH0[YQhcHWvYX6g[ZJjSjFiYYX0c5Bpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUS0JO69VQ>? M2rWcFE3PDhyMki0
COS7 M4f1SGZ2dmO2aX;uJGF{e2G7 NIjzdYs6PiEQvF2= M3exdWlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl M1vx[|E4OTd6N{Ky
A549 MlK2R5l1d3SxeHnjJGF{e2G7 NV3ifGs4QTZiaB?= M{n4UGROW09? M3OxXWlEPTB;MUOuOVkh|ryP MnfKNVg{OTN6MEe=
K562 NYDLRoMzS3m2b4TvfIlkKEG|c3H5 MW[5OkBp NFmzOI1FVVOR MUPJR|UxRTlwM{[g{txO MmXQNVg{OTN6MEe=
MDA-MB-231 NFTQ[ZdEgXSxdH;4bYMhSXO|YYm= MnP4PVYhcA>? NVjvXWNoTE2VTx?= NFvOPFRKSzVyPUO2MlYyKM7:TR?= MX2xPFMyOzhyNx?=
MOLT4 M3fHS2N6fG:2b4jpZ{BCe3OjeR?= NVHlb4RvQTZiaB?= MnLjSG1UVw>? M2DxRmlEPTB;MUWuNFIh|ryP NUi0dllGOTh|MUO4NFc>
MCF7 NV3qPIhTS3m2b4TvfIlkKEG|c3H5 NFvzW5c2OCEQvF2= NFX0TWI4OiCq MkXGTWM2OD1zMj6wOUDPxE1? MmXPNVg4PzF6MUm=
BT474 NVLWRWhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX:3NkBp MWPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO MnmxNVkxOjh2MkS=
HN5 NVnzTJlPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XWelczKGh? NXntdVR6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkB4cXSqIFXDOVAhd2ZiMD6wNUDPxE1? M2WxU|E6ODJ6NEK0
BA/F3 NWnCeXV3S3m2b4TvfIlkKEG|c3H5 MVG3NkBp M1:2VmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> MkW3NVkzOzl{Mkm=
NIH3T3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYG3NkBp NYX2VHNtT3Kxd4ToJIlvcGmkaYTpc44hd2ZibX;1d4UhVkmKL{PUN{Bk\WyuczDlfJBz\XO|aX7nJIVzSjJiZ3Xu[UB4cXSqIFnDOVAhd2ZiMD61OEDPxE1? MXSxPVY3PTN5Nx?=
MCF7 MX NFfiVI9HfW6ldHnvckBCe3OjeR?= M1P1WFMxKG2rbh?= NULGVmRlUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= MYixPVk{Ojl4MB?=
A431 M376[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUCxNEDPxE1? MUe3NkBp MoLES2k2OD1yLkWxOEDPxE1? M3nRdlE6QTZ7NE[1
A431 Mn73T4lv[XOnIFHzd4F6 M{LhWVAvOSEQvF2= M2fB[VEhcA>? NYTZO3hqUW6qaXLpeIlwdiCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? M{jGcVE6QTZ7NE[1
A431 MmfsT4lv[XOnIFHzd4F6 NW[3fGhJOTByIN88US=> MoLSNVAhdWmw NXPLc4Q2WHKxbH;u[4VlKGmwaHnibZRwenliYXP0bZZqfHlib3[gSWdHNWmwZIXj[YQhTUeIUjDwbI9{eGixconsZZRqd25? NEfzVVkyQTl4OUS2OS=>
Sf9 NYnCdnZpU2mwYYPlJGF{e2G7 NXTVN5NYOTBibXnu MlTrSG1UVw>? MXrJcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP M4LQO|IxODV4NEK1
NCI-H1975 M2C3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWi1JO69VQ>? MlLkO|IhcA>? Mm\MTWM2OD16LkK2JO69VQ>? M3LSWVIxOTVzNkew
NCI-H1975 MofZT4lv[XOnIFHzd4F6 MlTmNVAh|ryP NIewR5JKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? MoHFNlAyPTF4N{C=
NCI-H1975 MY\GeY5kfGmxbjDBd5NigQ>? MkXxNVAh|ryP M{[2XGlvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NVnKXHJQOjBzNUG2O|A>
NCI-H1975 NVzJOXZiTnWwY4Tpc44hSXO|YYm= NVfCOWdEPSEQvF2= MXS0PEBp NVr2V4gxUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2VvMx?= NV\jdIhLOjBzNUG2O|A>
A375 Mn\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1[5Z|Q5KGh? MoTtTWM2OD1{MD61OUDPxE1? MW[yNFMxPDV|Nx?=
SMMC7721 NIHmOWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX20PEBp MnuyTWM2OD1|Mz6xNkDPxE1? NEO1VGwzODNyNEWzOy=>
DU145 NGDYUXhEgXSxdH;4bYMhSXO|YYm= M1j3XlUh\A>? MWHEUXNQ NFPMUlZKSzVyPUSyMlE4KM7:TR?= MXSyNFQ3PjV3NR?=
MIAPaCa2 NGO5WnlEgXSxdH;4bYMhSXO|YYm= MorEOUBl M3Th[GROW09? M3G1fGlEPTB;NUCuOlIh|ryP NEjkZ3ozODR4NkW1OS=>
PANC1 MUfDfZRwfG:6aXOgRZN{[Xl? MWW1JIQ> NWr5XFdnTE2VTx?= NXGzZmQ5UUN3ME20NE41OiEQvF2= MmHYNlA1PjZ3NUW=
PC9 M1vtW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFXHSnIu\GWoaXPp[Y51KGi3bXHuJHBEQSClZXzsd{B4cXSqIFXDOVAhd2ZiMD6wNVch|ryP MVyyNVIxQDhyMh?=
PC9 NYS5bWVsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fVcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[5Jwf3SqLYLld4l{fGGwdDDoeY1idiCSQ{mgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEV5NE\fRVc2OC:WN{mwUUBufXSjboSge4l1cCCHQ{WwJI9nKDdizszN MXqyNVIxQDhyMh?=
PC3 M{frdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XNe|ExKM7:TR?= NV74XmNZPzJiaB?= NETIOlFFVVOR Mn;5TWM2OD15LkSg{txO MXGyNVM2OzV2Nh?=
MDA-MB-468 NIPjNYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIS3[3YyODBizszN NFTjNIw1QCCq M2Hxb2ROW09? M{HuPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 MkXCNlE1Ojl4M{K=
Plasmodium falciparum NUO0WphjeUiWUzDwdo9ncWyrbneg[o9zKGmwaHnibZRwenNib3[gVIxie22xZHn1cUBn[WylaYDhdpVuKHC{b3zp[oVz[XSrb36= NUm3eplOOjF6MUewOFU>
NCI-H1993 NWnwUlJ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnQfnQ4OiCq NGH5SmxFVVOR M37OdGlEPTB;MD6xJO69VQ>? MUSyNVkyPjR|Mx?=
NCI-H292 M2n6TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfQO|IhcA>? MmDNSG1UVw>? M3fkNGlEPTB;MD6wNkDPxE1? MXKyNVkyPjR|Mx?=
A431 NGnoU5dMcW6jc3WgRZN{[Xl? NH\wTYIzKGh? NYW5dVFYTE2VTx?= MkH2TY5pcWKrdHnvckBw\iCHR1\SJJBpd3OyaH;yfYxifGmxbjDpckBGT0Zvc4TpcZVt[XSnZDDoeY1idiCDNEOxJJdqfGhiSVO1NEBw\iByLkCwNUDPxE1? MWCyNlExOTF|Mh?=
MCF7 M33uPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm[yNVAxyqEQvHevcWw> MXi0PEBp MmrqSG1UVw>? M37HZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> M1KwOlIzOTF7MUOw
BT474 NVSxR5FHS3m2b4TvfIlkKEG|c3H5 Mn\kNVAh|ryP MV:0PEBp MlW2R5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= MUWyNlE{QDNyOB?=
MCF7 MV;DfZRwfG:6aXOgRZN{[Xl? M1HvNVExKM7:TR?= MVe0PEBp MV\DfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKE2FRkegZ4VtdHNib4\ldk1mgHC{ZYPzbY5oKEW{YlKyJINmdGy| M4LZfFIzOTN6M{C4
HCC827 NH;nXXFMcW6jc3WgRZN{[Xl? NX74VYxzOC5zMkWg{txO NVLVUoVJOjRiaB?= NYT1NZBCTE2VTx?= M3zzb2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MXqyNlM{QTN2Mh?=
HCC827 NWnpS5dsU2mwYYPlJGF{e2G7 MlPTNE4yOjVizszN MWKyOEBp NEG1fHZFVVOR NFPwfJZKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= NHz3VFUzOjN|OUO0Ni=>
HCC827 NWi1W5R2U2mwYYPlJGF{e2G7 NYfvdY9tOC5zMkWg{txO NE\rXlMzPCCq NEjWOXFFVVOR MV3Jcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhSWu2IIDoc5NxcG:{eXzheIlwdg>? MnL2NlI{Ozl|NEK=
A431 M1zKO2tqdmG|ZTDBd5NigQ>? MoqyNVAxKM7:TR?= MlLON|AhdWmw NVHBRoJ3UW6qaXLpeIlwdiCxZjDFS2ZTKGSncnn2[YQh\nKxbTDoeY1idiCDNEOxJINmdGxibXXtZpJidmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyb3z5MWdtfS2WeYKgdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAyNjB6IN88US=> MoXPNlI3QTh5OEK=
H460 NVS2fVR{S3m2b4TvfIlkKEG|c3H5 NEPhdY44OiCq M1nnOGlEPTB;NT61PUDPxE1? M{nZUlIzPzJ5NES5
HT-29 MojuR5l1d3SxeHnjJGF{e2G7 NGrYbok4OiCq NFfpdmpKSzVyPUOuN|Yh|ryP Mo[1NlI4Ojd2NEm=
SGC7901 NUS0WlAyS3m2b4TvfIlkKEG|c3H5 NYPsNmpYPzJiaB?= MmC0TWM2OD1zMD6yOkDPxE1? M1vCZVIzPzJ5NES5
A498 NW\HbmpiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3RZpZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= NGnxWnAzOjhzOEi0PC=>
Calu3 MWnDfZRwfG:6aXOgRZN{[Xl? M4rLOVExKM7:Zz;tUC=> M{fxelczKGh? NFTEVJZKSzVyPUGuOkDPxE1? M1X5RlI{OTF4MU[4
FADU M1zyUWN6fG:2b4jpZ{BCe3OjeR?= NX\4Z5pzOTBizsznM41N NVPGNmVOPzJiaB?= MoDuTWM2OD1yLkC1JO69VQ>? Mon6NlMyOTZzNki=
HCC827 NELw[5ZEgXSxdH;4bYMhSXO|YYm= NVntd5VTOTBizsznM41N MVq3NkBp MWLJR|UxRTBwMECxOUDPxE1? NFracJMzOzFzNkG2PC=>
PC9 M1rqR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXhNVAh|rypL33M MkTlO|IhcA>? MXXJR|UxRTBwMECxOEDPxE1? MoqyNlMyOTZzNki=
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A431 MmrYR5l1d3SxeHnjJGF{e2G7 NX3qSI1rPzJiaB?= MnT2SG1UVw>? NHfFR|ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPDNzIHPlcIx{KGW6cILld5NqdmdiRVfGVkB4cXSqIFnDOVAhd2ZiMD61OUDPxE1? M{TCSlI1PDFzMUKz
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NCI-H1975 NHv4VHhHfW6ldHnvckBCe3OjeR?= Mo\QNVAh|ryP M3fLNlghcA>? MlfHSG1UVw>? M2LnfYRw\XNibn;0JIlvcGmkaYTpJGVITlJiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEWUSzDwbI9{eGixconsZZRqd25? NILiO2szOzFzNkG2PC=>
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JVM-3 MmPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTJwM{S2OFMh|ryP NGraR3pUSU6JRWK=
NCI-H2170 NFm3SGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfqSIpKSzVyPUKuN|Q4QDNizszN NGHsN4FUSU6JRWK=
EW-18 NHe3eVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TtcWlEPTB;Mj6zOVQ4OSEQvF2= NUf1NnBvW0GQR1XS
KU812 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTJwM{[3O|kh|ryP MmTuV2FPT0WU
NY NEnRNXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknMTWM2OD1{LkO3Nlch|ryP M1LKR3NCVkeHUh?=
HTC-C3 M176NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrGTWM2OD1{LkSyNlY4KM7:TR?= NHvsRmNUSU6JRWK=
CFPAC-1 NX7S[FNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHlUW5KSzVyPUKuOFM5PjRizszN M{S4WnNCVkeHUh?=
HH MoLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HTfmlEPTB;Mj60PVg2PCEQvF2= MVzTRW5ITVJ?
MKN7 M1zYfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rEd2lEPTB;Mj62Nlc1QCEQvF2= NIPjNINUSU6JRWK=
TE-8 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LJeGlEPTB;Mj62N|Y2OyEQvF2= NES1NJVUSU6JRWK=
MC-IXC NYTH[HZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJwNkS0OVMh|ryP NGrjNmpUSU6JRWK=
DMS-79 M4HGSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLRfJNKSzVyPUKuOlkzQDRizszN MVTTRW5ITVJ?
Detroit562 NWrDOZZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXZeoFIUUN3ME2yMlY6QDR3IN88US=> NVfzdlhsW0GQR1XS
HuP-T4 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TMUWlEPTB;Mj63NlgyQCEQvF2= NXG4OIdCW0GQR1XS
QIMR-WIL NHHZdmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPoXVZKSzVyPUKuO|U6PTJizszN NV\tdZFoW0GQR1XS
BB65-RCC NHjvZ|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTJwOEC3NVMh|ryP Mnv1V2FPT0WU
PC-3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rIT2lEPTB;Mj64N|MyPyEQvF2= MYjTRW5ITVJ?
OVCAR-3 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zvcGlEPTB;Mj64OVU6PCEQvF2= NITQfGxUSU6JRWK=
KYSE-510 NU\zUGdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;J[JFKSzVyPUKuPFY4QDZizszN NFf1N4dUSU6JRWK=
HC-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDDTWM2OD1{Lkm0NFEzKM7:TR?= MnL0V2FPT0WU
NCI-H1693 NUXSdZZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEe4[mNKSzVyPUKuPVU5QTdizszN NWS2N3dZW0GQR1XS
DU-4475 MkHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJwOUmwOVIh|ryP MUPTRW5ITVJ?
COLO-680N MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPMXGhKSzVyPUOuNFA1QTVizszN M4HCU3NCVkeHUh?=
MN-60 NUfIWpdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4niNmlEPTB;Mz6wOFk4PCEQvF2= MVPTRW5ITVJ?
BCPAP MmPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[4THFsUUN3ME2zMlA5Ojl3IN88US=> M{WzdnNCVkeHUh?=
647-V NFTjVZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDTTWM2OD1|LkG2NlIh|ryP M2PyTXNCVkeHUh?=
HT-1376 NFSxdZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTNwMkC1NFYh|ryP NYrpdnk4W0GQR1XS
NCI-H1793 MlvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmn1TWM2OD1|LkKxNFk5KM7:TR?= MXjTRW5ITVJ?
SW1463 NUm5RpFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4[xTGlEPTB;Mz6yOlE6OiEQvF2= MV3TRW5ITVJ?
NCI-H727 M{TpNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTNwM{i1PFQh|ryP NFT5PVRUSU6JRWK=
LB771-HNC NHP6TWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXUSFJKSzVyPUOuN|kyOjZizszN Ml7LV2FPT0WU
D-542MG NU\VXFd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHUTWM2OD1|LkS0NlIzKM7:TR?= NU\CZmlOW0GQR1XS
NTERA-S-cl-D1 NV\G[lBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7ETWM2OD1|LkS0PVU4KM7:TR?= NUSwT20{W0GQR1XS
C8166 NWrLO2YzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXnTWM2OD1|LkS2OVIyKM7:TR?= NF\ZS4dUSU6JRWK=
639-V NGPOOZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7aWldKSzVyPUOuOVc4QTJizszN MX\TRW5ITVJ?
TE-1 NHvROFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTTfZI5UUN3ME2zMlU6OTZ7IN88US=> NWPFNnlKW0GQR1XS
OAW-28 M1P2Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTNwNUm0NlYh|ryP M3;uT3NCVkeHUh?=
SK-LU-1 M1;Bemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXK5PXc3UUN3ME2zMlYxPjR3IN88US=> NH;3XGtUSU6JRWK=
CaR-1 Mn\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXxZXBKSzVyPUOuOlExPDhizszN NWXFW|V{W0GQR1XS
A2780 Mn;XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEj3[41KSzVyPUOuOlQxQDlizszN NXr4eYtvW0GQR1XS
C2BBe1 M4G1SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTNwNkS0NFch|ryP M3riOXNCVkeHUh?=
SK-MES-1 NX74dmRET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LmN2lEPTB;Mz62OFYyPCEQvF2= M2\pWHNCVkeHUh?=
PANC-10-05 NGTZNlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml[wTWM2OD1|LkeyNFczKM7:TR?= NVXSNG9GW0GQR1XS
J-RT3-T3-5 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7jTWM2OD1|Lke1OFA3KM7:TR?= NYT3U3NMW0GQR1XS
ES4 MlqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\ZUGdRUUN3ME2zMlgzOjh4IN88US=> NHzpb2hUSU6JRWK=
NCI-H28 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{G4dGlEPTB;Mz64Nlk3OSEQvF2= MmO4V2FPT0WU
NCI-H1155 M2rIe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfyTWM2OD1|Lki0NVU6KM7:TR?= MUPTRW5ITVJ?
KURAMOCHI Ml3US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWn6WYVCUUN3ME2zMlg3ODV4IN88US=> MVjTRW5ITVJ?
NCI-H69 NV\JXXhHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIn1Z3pKSzVyPUOuPVAyPzhizszN MlvwV2FPT0WU
IST-MES1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD5PIhsUUN3ME2zMlk1ODJ7IN88US=> NV6zR|VmW0GQR1XS
NH-12 M4jPe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XxN2lEPTB;Mz65OFE5PyEQvF2= NUflOWNuW0GQR1XS
JVM-2 M{C2Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHewV3FKSzVyPUOuPVQ4PTJizszN NUPBWm8yW0GQR1XS
EW-16 NETuRpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTNwOUW1O|ch|ryP MVTTRW5ITVJ?
NCI-H441 MmLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTNwOUewNVQh|ryP NGnX[YNUSU6JRWK=
RCC10RGB M{jrWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTYU3RyUUN3ME20MlAyOjR4IN88US=> NFG2T|JUSU6JRWK=
TCCSUP MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXIPVhKSzVyPUSuNVExPSEQvF2= NG\1PHhUSU6JRWK=
HL-60 NGH6T4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTRwMUKyOFEh|ryP MoX1V2FPT0WU
EW-11 NVrCW417T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzNcolmUUN3ME20MlE{OjZzIN88US=> NWnFXId2W0GQR1XS
KARPAS-299 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7CTWM2OD12LkOxNFE{KM7:TR?= NV;JPHJ4W0GQR1XS
SNU-C2B NFHMcWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\STWM2OD12LkO0Olg3KM7:TR?= M13zW3NCVkeHUh?=
LU-139 NVXpRXVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTRwM{eyOFch|ryP NYe1VGliW0GQR1XS
HCC1937 NVPTfYhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTRwM{e5PVMh|ryP MnPCV2FPT0WU
GR-ST MkDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWK3PY5wUUN3ME20MlQ{OThizszN MYnTRW5ITVJ?
SK-OV-3 NUD4TZBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTRwNE[wOFIh|ryP Mof6V2FPT0WU
A172 M2PvWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17QT2lEPTB;ND61OVUzOSEQvF2= MlrnV2FPT0WU
NB10 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrmTWM2OD12LkW5O|gyKM7:TR?= NIToTY1USU6JRWK=
P30-OHK NHO4OXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTRwOEG5Nlkh|ryP NWr6e29TW0GQR1XS
KYSE-520 NFHLenhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDnTWM2OD12Lki4NFE5KM7:TR?= NIPBSpFUSU6JRWK=
KNS-62 MkjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFf6UoFKSzVyPUSuPVA5ODdizszN NWLMc5VyW0GQR1XS
ES1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEn0ZYlKSzVyPUSuPVYxOzVizszN MnSyV2FPT0WU
KOSC-2 M4K5[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoX5TWM2OD12Lkm5PVU6KM7:TR?= NG\xSHpUSU6JRWK=
TE-9 NXHrTVIxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHjVopKSzVyPUWuNFE2OjVizszN NI\PfVlUSU6JRWK=
NCI-H1838 NHXhSWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HHRWlEPTB;NT6wNlA4PiEQvF2= NF7JcoRUSU6JRWK=
KP-N-YN MlLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PvVGlEPTB;NT6xNlU6OSEQvF2= NYLDe4RuW0GQR1XS
ALL-PO MnT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;JdGlEPTB;NT6yNlU5PyEQvF2= M2j0bXNCVkeHUh?=
NOMO-1 M{fNfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nXO2lEPTB;NT6yN|E4PiEQvF2= MnzZV2FPT0WU
CGTH-W-1 NXLLWmR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml36TWM2OD13LkO4OVg1KM7:TR?= MYrTRW5ITVJ?
ATN-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHQSotKSzVyPUWuOFEyQDRizszN NHLWfJNUSU6JRWK=
AGS M1HyfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfhNpJHUUN3ME21MlQ6PjF6IN88US=> MYDTRW5ITVJ?
SW13 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXDenVTUUN3ME21MlU{OjZ3IN88US=> MVrTRW5ITVJ?
SW1990 NGTnZ29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fOT2lEPTB;NT62NlI5PSEQvF2= M{jQ[HNCVkeHUh?=
BFTC-905 M4TNNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\CXYVKSzVyPUWuOlU5PzhizszN NEfBcnVUSU6JRWK=
RMG-I MkTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTVwNkW5OFIh|ryP M4PpR3NCVkeHUh?=
HD-MY-Z NVX5WY0yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\HT5ZjUUN3ME21MlY5PThzIN88US=> MnrVV2FPT0WU
HCC1187 M1n0fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTVwN{GwN|Ih|ryP NGr0SIpUSU6JRWK=
HCC2998 MlfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLGTWM2OD13LkexPFc4KM7:TR?= NV;QPZhJW0GQR1XS
RT-112 NIrTOZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnDTWM2OD13LkmwNlQ2KM7:TR?= NYHV[phlW0GQR1XS
CAKI-1 Mo\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrDTWM2OD14LkCwPVg2KM7:TR?= MVjTRW5ITVJ?
HDLM-2 NXKxWlhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTZwMEG0PFkh|ryP NHvB[o1USU6JRWK=
TE-10 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI[4V5dKSzVyPU[uNFQ5OzNizszN NWXzcnRrW0GQR1XS
KE-37 MlXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfYeJRKSzVyPU[uNFczPThizszN NYrLZWk1W0GQR1XS
RCM-1 NXPwZWJyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTZwMEe3NVkh|ryP M1LjRXNCVkeHUh?=
Mewo NFLWcnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLLTWM2OD14LkGxOFg4KM7:TR?= NF3NSoVUSU6JRWK=
L-428 M1LLbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\YdVNOUUN3ME22MlE5ODN5IN88US=> NFfRNmhUSU6JRWK=
8-MG-BA MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVK0PHMyUUN3ME22MlE6Ozd7IN88US=> NEjVPJZUSU6JRWK=
SK-MEL-28 NU\kZW17T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlq1TWM2OD14LkKwPVY4KM7:TR?= M1f3XHNCVkeHUh?=
VA-ES-BJ NVfTU5MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnn4TWM2OD14LkOwOFYzKM7:TR?= NHr2OoJUSU6JRWK=
CAL-120 NH\1Z5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLCTWM2OD14LkS1NVYzKM7:TR?= NXf2d49YW0GQR1XS
GAMG MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPOWXNKSzVyPU[uOVA3OiEQvF2= NUW1dHhjW0GQR1XS
MSTO-221H M3;nXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYqzSG17UUN3ME22MlUyOTdizszN MoTRV2FPT0WU
GCT M3OxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTZwNUOzPFch|ryP MVnTRW5ITVJ?
SH-4 NYnZRmc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVS1U4JyUUN3ME22MlU5QDR2IN88US=> MX3TRW5ITVJ?
Calu-3 MoLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWT6PWdJUUN3ME22MlY2PTZ6IN88US=> NFnFNGRUSU6JRWK=
SK-HEP-1 M33mWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LOT2lEPTB;Nj63NVc2OyEQvF2= M1vDT3NCVkeHUh?=
PFSK-1 M2DzT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVqzNGFrUUN3ME22Mlc{PDd6IN88US=> NHXB[2dUSU6JRWK=
NB12 NUH5bmhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnflTWM2OD14Lke4OFA1KM7:TR?= NYH3e3pyW0GQR1XS
A388 NVuzd5pUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fYOmlEPTB;Nj64PVUzKM7:TR?= M1TmXnNCVkeHUh?=
KLE NU\FdHdOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTZwOU[1NVYh|ryP Mn[5V2FPT0WU
TE-11 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlf6TWM2OD15LkC0PVY4KM7:TR?= NYrscnRiW0GQR1XS
NCI-H2405 MkfTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTdwMEizPVMh|ryP M3\vZXNCVkeHUh?=
OVCAR-4 Mn3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnlTWM2OD15LkG1N|Uh|ryP MmThV2FPT0WU
KP-N-YS M2TtZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrXNVRKSzVyPUeuNVgyPCEQvF2= NF\odWNUSU6JRWK=
NOS-1 NGTLTlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33lb2lEPTB;Nz6yOVgzQCEQvF2= MYHTRW5ITVJ?
DMS-114 NEPPNo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfSTWM2OD15LkK5OlMyKM7:TR?= MmrzV2FPT0WU
NCI-H2030 NHrYeVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLJTWM2OD15LkOzOlk5KM7:TR?= NGHPR3RUSU6JRWK=
HEL NEHj[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTdwM{e1NlEh|ryP MknOV2FPT0WU
CAL-62 NFLxN45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33kOWlEPTB;Nz6zPVg3PiEQvF2= MVfTRW5ITVJ?
COLO-668 M1zVPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3tPIVKSzVyPUeuOFM6PjdizszN Ml;qV2FPT0WU
SKG-IIIa M2j3TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzNTWM2OD15LkS1N|gyKM7:TR?= MVjTRW5ITVJ?
HCC1419 NHrjc|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTdwNE[4N|Mh|ryP NVr0V3RUW0GQR1XS
OS-RC-2 Mo\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVz6e5BWUUN3ME23MlQ6QDl2IN88US=> MUXTRW5ITVJ?
COLO-829 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInF[ldKSzVyPUeuOlY1PjNizszN NHrWTJVUSU6JRWK=
NB13 MmjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fWNmlEPTB;Nz64NlY3PyEQvF2= MnHFV2FPT0WU
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T98G NUDRdVRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTlR4RKSzVyPUO3Mlg3OzVizszN MYrTRW5ITVJ?
NB14 M4DJbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTN6LkS1OFQh|ryP MofvV2FPT0WU
D-423MG MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoT2TWM2OD1|OD60PFY6KM7:TR?= M13Pd3NCVkeHUh?=
NCI-H510A NGfXU4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{ew[mlEPTB;M{iuOlQ2OSEQvF2= MYfTRW5ITVJ?
NMC-G1 NU\sSpo4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LGZWlEPTB;M{iuO|IzOiEQvF2= MlO4V2FPT0WU
SW948 M2XBVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmn3TWM2OD1|OD64PVQ6KM7:TR?= NXfZcoZPW0GQR1XS
MFM-223 NWrvdWpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rGUGlEPTB;M{iuPVQ{OSEQvF2= MoXYV2FPT0WU
PAN1 MoPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\POZNKSzVyPUO4Mlk6OTlizszN NHHU[WJUSU6JRWK=
MZ7-mel M4jWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLlTWM2OD1|OT6xNVk6KM7:TR?= NFrhXGFUSU6JRWK=
COLO-679 M2f5SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLsVld7UUN3ME2zPU46PzB6IN88US=> MYLTRW5ITVJ?
SW872 NIrDRo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTRyLkKwN|Eh|ryP Mmf2V2FPT0WU
HT-1197 M1qxcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vXfWlEPTB;NECuNlg3QSEQvF2= MW\TRW5ITVJ?
ES6 NULkWVVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTRyLkS1OVIh|ryP NILtcmlUSU6JRWK=
SNU-387 NIrBOXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fIV2lEPTB;NECuPFI{QCEQvF2= M1P6RXNCVkeHUh?=
T47D M{CxXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXSXYJKSzVyPUSxMlAyQTJizszN NV7ke|FVW0GQR1XS
G-402 MonkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTRzLkC4NFkh|ryP M1vWTnNCVkeHUh?=
MZ1-PC NFjFWIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEn0RpRKSzVyPUSxMlgyKM7:TR?= MX3TRW5ITVJ?
SW1417 NXXododqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDpTVBKSzVyPUSyMlMxOyEQvF2= NXfSOXNiW0GQR1XS
RERF-LC-MS MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTR{LkWwPFch|ryP M1HlNXNCVkeHUh?=
SF539 NICxRWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTR{LkW1Nlkh|ryP MWPTRW5ITVJ?
ESS-1 MoXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnu3TWM2OD12Mz61OVU5KM7:TR?= NYW3cVc5W0GQR1XS
RO82-W-1 NXnCOIQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofGTWM2OD12Mz62NVU{KM7:TR?= MmfJV2FPT0WU
S-117 M13kSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nTd2lEPTB;NEOuOlk2QCEQvF2= MWnTRW5ITVJ?
SW756 NGLDNnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LTU2lEPTB;NEOuPFI1OiEQvF2= MVvTRW5ITVJ?
CP66-MEL NFTve5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLZbIVKSzVyPUS1MlcyPDNizszN Mlv2V2FPT0WU
H-EMC-SS MnvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPUZZUxUUN3ME20OU44Pjl|IN88US=> MWPTRW5ITVJ?
NCI-H2052 MoDlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3ifZJXUUN3ME20OU46ODJ5IN88US=> NEfmc3JUSU6JRWK=
SW684 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTR5LkCwPFEh|ryP MYrTRW5ITVJ?
HCC1569 MnGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrSe25KSzVyPUS3MlE3PThizszN MlzMV2FPT0WU
KGN MnjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrte5lKSzVyPUS3MlM{OTFizszN NF;tbmFUSU6JRWK=
LCLC-103H M1e5WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPLTWM2OD12Nz60OFA1KM7:TR?= MXHTRW5ITVJ?
SW780 NFnCT5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULvSJBGUUN3ME20O{43OzVzIN88US=> NH3RSJpUSU6JRWK=
NCI-H1703 NYTXSGRPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYK5W3RkUUN3ME20PE4{OzV{IN88US=> MlzlV2FPT0WU
HCC1395 MlHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPYZlRKSzVyPUS4Mlc1OzJizszN M4e0bHNCVkeHUh?=

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

お薦めの試験操作(参考用のみ)

細胞試験: [1]
+ 展開
  • 細胞株: NR6, NR6M and NR6W cells
  • 濃度: 0-2 μM
  • 反応時間: 72 hours
  • 実験の流れ: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (参考用のみ)
動物試験:[3]
+ 展開
  • 動物モデル: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • 製剤: 0.5% polysorbate
  • 投薬量: 100 mg/kg
  • 投与方法: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます:
1% CMC Na
混合させたのち直ちに使用することを推奨します。
30mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 446.90
化学式

C22H24ClFN4O3

CAS No. 184475-35-2
保管
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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