Gefitinib (ZD1839)

製品コードS1025

Gefitinib (ZD1839)化学構造

分子量(MW):446.90

Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。

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文献中の使用例(91)

カスタマーフィードバック(12)

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

製品安全説明書

EGFR阻害剤の選択性比較

生物活性

製品説明 Gefitinib (ZD-1839)は一種のEGFR阻害剤で、NR6wtEGFRとNR6Wの細胞中のTyr1173、Tyr992、Tyr1173とTyr992に作用する時のIC50値が37 nM、37nM、26 nMと57 nMにそれぞれ分かれることです。
特性 A potent EGFR tyrosine kinase inhibitor.
ターゲット
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外試験

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M{XqcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFO2RWtKSzVyPUCuNFU1KM7:TR?= MXOxOlUyPjR5Mx?=
NIH3T3 NIPrcY1HfW6ldHnvckBCe3OjeR?= NU\ONIFROC5{IN88US=> MkT0NkBp NVnYVGFuTE2VTx?= M{HRNWlvcGmkaYTpc44hd2ZiRVfGJJN1cW23bHH0[YQhcHWvYX6g[ZJjSjFiYYX0c5Bpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUS0JO69VQ>? MVuxOlQ5ODJ6NB?=
COS7 NVS1bVR2TnWwY4Tpc44hSXO|YYm= NXq1WlRsQTZizszN NXvkWmo3UW6qaXLpeIlwdiCxZjDoeY1idiCMQVuyJHY3OTeIIH31eIFvfCCneIDy[ZN{\WR? NGDjTY0yPzF5OEeyNi=>
A549 MkjTR5l1d3SxeHnjJGF{e2G7 NV\PRWZSQTZiaB?= M3;EfWROW09? MV3JR|UxRTF|LkW5JO69VQ>? M3j2b|E5OzF|OEC3
K562 Mo[3R5l1d3SxeHnjJGF{e2G7 NVLuclRMQTZiaB?= M3zvXmROW09? NH[wV4lKSzVyPUmuN|Yh|ryP MV6xPFMyOzhyNx?=
MDA-MB-231 M4fqTmN6fG:2b4jpZ{BCe3OjeR?= MX:5OkBp MUTEUXNQ M2X3dGlEPTB;M{[uOlEh|ryP NYnT[YxmOTh|MUO4NFc>
MOLT4 NF75VG5EgXSxdH;4bYMhSXO|YYm= NGjSW4I6PiCq NGrJRY1FVVOR M2f1emlEPTB;MUWuNFIh|ryP MUGxPFMyOzhyNx?=
MCF7 M4niNWN6fG:2b4jpZ{BCe3OjeR?= M1nMTFUxKM7:TR?= NWX1S3JVPzJiaB?= M1;MRWlEPTB;MUKuNFUh|ryP Ml3ENVg4PzF6MUm=
BT474 M1TVUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnHT3ZGPzJiaB?= M4rEPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS0O|Qh[2WubIOgc5ZmemW6cILld5NqdmdiRWLCZlIhf2m2aDDFR|UxKG:oIECuN{DPxE1? MUmxPVAzQDR{NB?=
HN5 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVS3NkBp MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiQNTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghTUN3MDDv[kAxNjBzIN88US=> M4\J[FE6ODJ6NEK0
BA/F3 MXrDfZRwfG:6aXOgRZN{[Xl? M1vSZlczKGh? MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCRU9HOyClZXzsd{BmgHC{ZYPzbY5oKEWJRmKgUFg2QFJibYX0ZY51KHerdHigTWM2OCCxZjCwMlAyODhizszN MV[xPVI{QTJ{OR?=
NIH3T3 M2PMPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjtco5yPzJiaB?= M3qxZ2dzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUC9|VEOgZ4VtdHNiZYjwdoV{e2mwZzDldmIzKGenbnWge4l1cCCLQ{WwJI9nKDBwNUSg{txO MYOxPVY3PTN5Nx?=
MCF7 MX MXXGeY5kfGmxbjDBd5NigQ>? MYSzNEBucW5? NHTNbIRKdmirYnn0bY9vKG:oIFLDVnAh\XiycnXzd4VlKHerdHigTWM2OCCxZjCxMlEh|ryP MYWxPVk{Ojl4MB?=
A431 MkjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjGbYxsOTBizszN NEW3TFA4OiCq NIfLcmZIUTVyPUCuOVE1KM7:TR?= M1W3c|E6QTZ7NE[1
A431 NU\IU2lYU2mwYYPlJGF{e2G7 MWqwMlEh|ryP NVz6Z3RQOSCq M1zGWmlvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> M3rMSlE6QTZ7NE[1
A431 MmraT4lv[XOnIFHzd4F6 NFfWZ2oyODBizszN MWqxNEBucW5? MkX5VJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> NIT2ZlkyQTl4OUS2OS=>
Sf9 MoL3T4lv[XOnIFHzd4F6 MnrRNVAhdWmw NVvTZ|JuTE2VTx?= NIHUVJRKdmirYnn0bY9vKG:oIFXHSnIh\XiycnXzd4VlKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhemWlZYD0c5Ih[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzNizszN NX7RTY9QOjByNU[0NlU>
NCI-H1975 M3vIS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;NUII2KM7:TR?= M1O4SlczKGh? MkTTTWM2OD16LkK2JO69VQ>? MW[yNFE2OTZ5MB?=
NCI-H1975 MV7LbY5ie2ViQYPzZZk> M3zkSVExKM7:TR?= MlvKTY5pcWKrdHnvckBw\iCHR1\SJIF2fG:yaH;zdIhwenmuYYTpc44> NEXNSWQzODF3MU[3NC=>
NCI-H1975 NXjv[GJTTnWwY4Tpc44hSXO|YYm= MnfpNVAh|ryP M3TaeWlvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NWjuR5YzOjBzNUG2O|A>
NCI-H1975 M3roPGZ2dmO2aX;uJGF{e2G7 MX:1JO69VQ>? MnzyOFghcA>? M3HTUWlv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? M{K4cFIxOTVzNkew
A375 MlLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUe0PEBp NUPyVGlrUUN3ME2yNE42PSEQvF2= M2faV|IxOzB2NUO3
SMMC7721 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17EZ|Q5KGh? NEPQXoxKSzVyPUOzMlEzKM7:TR?= NVfLV4JiOjB|MES1N|c>
DU145 NVHz[WdTS3m2b4TvfIlkKEG|c3H5 MofFOUBl M3Lhd2ROW09? M1X5W2lEPTB;NEKuNVch|ryP M{DvWFIxPDZ4NUW1
MIAPaCa2 NFnsbolEgXSxdH;4bYMhSXO|YYm= NHf0W4Q2KGR? NYfubo9vTE2VTx?= NVL6OZFmUUN3ME21NE43OiEQvF2= NEjGZm0zODR4NkW1OS=>
PANC1 MnnNR5l1d3SxeHnjJGF{e2G7 M4fX[FUh\A>? MlPBSG1UVw>? NX\UV4pYUUN3ME20NE41OiEQvF2= NEm0OYYzODR4NkW1OS=>
PC9 NHXhV2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPnc2tCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN NFjwfHkzOTJyOEiwNi=>
PC9 NFXnU5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3K0dmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[5Jwf3SqLYLld4l{fGGwdDDoeY1idiCSQ{mgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEV5NE\fRVc2OC:WN{mwUUBufXSjboSge4l1cCCHQ{WwJI9nKDdizszN MWGyNVIxQDhyMh?=
PC3 NI\1b4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1T2W|ExKM7:TR?= M{j2R|czKGh? M4nPZmROW09? NXmzeJJEUUN3ME23MlQh|ryP MWSyNVM2OzV2Nh?=
MDA-MB-468 M2roN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PVZ|ExOCEQvF2= NGLXNmI1QCCq NUW1cWdSTE2VTx?= NFTkXmlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> M4fvSlIyPDJ7NkOy
Plasmodium falciparum NWHnS5RueUiWUzDwdo9ncWyrbneg[o9zKGmwaHnibZRwenNib3[gVIxie22xZHn1cUBn[WylaYDhdpVuKHC{b3zp[oVz[XSrb36= M3m0[VIyQDF5MES1
NCI-H1993 NITGT|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TKSFczKGh? Mn;wSG1UVw>? NVz4[Il6UUN3ME2wMlEh|ryP NXviOlA{OjF7MU[0N|M>
NCI-H292 MnS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{f1XlczKGh? NVXuRoNMTE2VTx?= MmDwTWM2OD1yLkCyJO69VQ>? NFewZogzOTlzNkSzNy=>
A431 MnjmT4lv[XOnIFHzd4F6 M3P5UVIhcA>? MXHEUXNQ NYjzTFNDUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= NWW5[o1zOjJzMEGxN|I>
MCF7 M{e3TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWKxNFDDqM7:Zz;tUC=> NYLvcFhDPDhiaB?= MUDEUXNQ M2rldmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> NITSRXAzOjFzOUGzNC=>
BT474 NFLkUopEgXSxdH;4bYMhSXO|YYm= MWmxNEDPxE1? NH[z[IQ1QCCq MXXDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| NFHQSnkzOjF|OEOwPC=>
MCF7 Mn;VR5l1d3SxeHnjJGF{e2G7 M2jDdlExKM7:TR?= M33rTFQ5KGh? M37ORWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= NUnuXWdMOjJzM{izNFg>
HCC827 NWHJRphsU2mwYYPlJGF{e2G7 NILEfYgxNjF{NTFOwG0> NIXFdpIzPCCq NYfsRoVGTE2VTx?= M13vcGlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MkfxNlI{Ozl|NEK=
HCC827 M4DQXmtqdmG|ZTDBd5NigQ>? M2rzXVAvOTJ3IN88US=> NU\JfVdHOjRiaB?= MnnsSG1UVw>? NVfBUVhkUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFXyb{BxcG:|cHjvdplt[XSrb36= NWHUOJlzOjJ|M{mzOFI>
HCC827 NYL3PW0xU2mwYYPlJGF{e2G7 Mn:wNE4yOjVizszN NGPlbJczPCCq MmXmSG1UVw>? M3nhRWlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w NXTzSlg2OjJ|M{mzOFI>
A431 M4PxO2tqdmG|ZTDBd5NigQ>? M3S5flExOCEQvF2= MoDDN|AhdWmw NH7KWFVKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN MYmyNlY6QDd6Mh?=
H460 NUjTemsyS3m2b4TvfIlkKEG|c3H5 NWHINHp{PzJiaB?= NHztT|ZKSzVyPUWuOVkh|ryP NIrhSYkzOjd{N{S0PS=>
HT-29 M1HYUmN6fG:2b4jpZ{BCe3OjeR?= M3vKelczKGh? MnezTWM2OD1|LkO2JO69VQ>? MnzVNlI4Ojd2NEm=
SGC7901 M{LZXmN6fG:2b4jpZ{BCe3OjeR?= NVrrem9xPzJiaB?= M3fFZmlEPTB;MUCuNlYh|ryP MUKyNlczPzR2OR?=
A498 NVL5[2VQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF2OUigZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKGGwZDDTVmMhf2m2aDDJR|UxKG:oIEiuOkDPxE1? NX\We5gxOjJ6MUi4OFg>
Calu3 NI\FW|BEgXSxdH;4bYMhSXO|YYm= MlnQNVAh|rypL33M M3Tpb|czKGh? M3Xi[GlEPTB;MT62JO69VQ>? NHrTeXQzOzFzNkG2PC=>
FADU MkXpR5l1d3SxeHnjJGF{e2G7 M2XsSVExKM7:Zz;tUC=> NIjld3k4OiCq NWj5OXVFUUN3ME2wMlA2KM7:TR?= MXuyN|EyPjF4OB?=
HCC827 NIfQbmdEgXSxdH;4bYMhSXO|YYm= MWKxNEDPxGdxbVy= M1PXV|czKGh? NGTSRVBKSzVyPUCuNFAyPSEQvF2= NV;KZWxsOjNzMU[xOlg>
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CFPAC-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzWZmN7UUN3ME2yMlQ{QDZ2IN88US=> Mn;JV2FPT0WU
HH NWrIOWpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M176[WlEPTB;Mj60PVg2PCEQvF2= MUXTRW5ITVJ?
MKN7 NIrEbIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnle|hIUUN3ME2yMlYzPzR6IN88US=> MkfYV2FPT0WU
TE-8 NGTWZ41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TSV2lEPTB;Mj62N|Y2OyEQvF2= M1zvOHNCVkeHUh?=
MC-IXC MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJwNkS0OVMh|ryP Ml3XV2FPT0WU
DMS-79 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLUN3p2UUN3ME2yMlY6Ojh2IN88US=> MWrTRW5ITVJ?
Detroit562 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\TUVdKSzVyPUKuOlk5PDVizszN M3eyXHNCVkeHUh?=
HuP-T4 MmPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTJwN{K4NVgh|ryP NUe4cWZCW0GQR1XS
QIMR-WIL NXfyVoM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjvXWw5UUN3ME2yMlc2QTV{IN88US=> Ml:wV2FPT0WU
BB65-RCC NVLuUYl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDxNXY6UUN3ME2yMlgxPzF|IN88US=> MnrRV2FPT0WU
PC-3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorPTWM2OD1{LkizN|E4KM7:TR?= NH7ufI5USU6JRWK=
OVCAR-3 NY\VbmJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnftTWM2OD1{Lki1OVk1KM7:TR?= NH3tNGJUSU6JRWK=
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HC-1 MoLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7XPGJ6UUN3ME2yMlk1ODF{IN88US=> NWjOfmZNW0GQR1XS
NCI-H1693 NEO4d3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvuZWtKSzVyPUKuPVU5QTdizszN MUfTRW5ITVJ?
DU-4475 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTJwOUmwOVIh|ryP M1H0VHNCVkeHUh?=
COLO-680N NH3xTnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzwe3ZKSzVyPUOuNFA1QTVizszN M3nwcnNCVkeHUh?=
MN-60 NVXtXmFLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlK3TWM2OD1|LkC0PVc1KM7:TR?= M164fnNCVkeHUh?=
BCPAP M3W2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHK2bJRKSzVyPUOuNFgzQTVizszN NXLhTlVLW0GQR1XS
647-V Ml\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke2TWM2OD1|LkG2NlIh|ryP MnmyV2FPT0WU
HT-1376 M2nxdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkO2TWM2OD1|LkKwOVA3KM7:TR?= MmqxV2FPT0WU
NCI-H1793 M1XUeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHwTWM2OD1|LkKxNFk5KM7:TR?= NXnyOIYyW0GQR1XS
SW1463 MnH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O2TGlEPTB;Mz6yOlE6OiEQvF2= NUDLfGZPW0GQR1XS
NCI-H727 M{fONmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NICwe4tKSzVyPUOuN|g2QDRizszN NGXQfIVUSU6JRWK=
LB771-HNC MmTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYf0UVBCUUN3ME2zMlM6OTJ4IN88US=> MnrhV2FPT0WU
D-542MG Mn7GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPrTWM2OD1|LkS0NlIzKM7:TR?= NYHXVZVpW0GQR1XS
NTERA-S-cl-D1 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojlTWM2OD1|LkS0PVU4KM7:TR?= MVrTRW5ITVJ?
C8166 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2[2eGlEPTB;Mz60OlUzOSEQvF2= NVLtb3FXW0GQR1XS
639-V NXrRVXJTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zpN2lEPTB;Mz61O|c6OiEQvF2= MUfTRW5ITVJ?
TE-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTNwNUmxOlkh|ryP MoWwV2FPT0WU
OAW-28 MkXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jsVWlEPTB;Mz61PVQzPiEQvF2= NIDkTWxUSU6JRWK=
SK-LU-1 NYW0c5NXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDjTWM2OD1|Lk[wOlQ2KM7:TR?= MYDTRW5ITVJ?
CaR-1 Ml3ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnQPIFXUUN3ME2zMlYyODR6IN88US=> NIfLbGhUSU6JRWK=
A2780 MkXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYSxfWR{UUN3ME2zMlY1ODh7IN88US=> M{f4OnNCVkeHUh?=
C2BBe1 MlLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEGzeXNKSzVyPUOuOlQ1ODdizszN M4KwUnNCVkeHUh?=
SK-MES-1 MmHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTNwNkS2NVQh|ryP M1jYcHNCVkeHUh?=
PANC-10-05 NYHh[|RJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7KTWM2OD1|LkeyNFczKM7:TR?= MX3TRW5ITVJ?
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NCI-H28 MlzhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXtSXFsUUN3ME2zMlgzQTZzIN88US=> M1TpenNCVkeHUh?=
NCI-H1155 M3rtPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTNwOESxOVkh|ryP MVnTRW5ITVJ?
KURAMOCHI NXvz[FQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTNwOE[wOVYh|ryP MVPTRW5ITVJ?
NCI-H69 NFvPSnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFf3cIVKSzVyPUOuPVAyPzhizszN MYHTRW5ITVJ?
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NH-12 NFv4cmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDBTWM2OD1|Lkm0NVg4KM7:TR?= NXH5e21MW0GQR1XS
JVM-2 NUHBTJAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTDXI5KSzVyPUOuPVQ4PTJizszN M1LzOXNCVkeHUh?=
EW-16 M2PlUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1u2NGlEPTB;Mz65OVU4PyEQvF2= MUTTRW5ITVJ?
NCI-H441 NEH0UFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLCc4dHUUN3ME2zMlk4ODF2IN88US=> MWjTRW5ITVJ?
RCC10RGB M1uwT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTRwMEGyOFYh|ryP MXrTRW5ITVJ?
TCCSUP MnPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFniOIJKSzVyPUSuNVExPSEQvF2= MWnTRW5ITVJ?
HL-60 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTRwMUKyOFEh|ryP NITrOoJUSU6JRWK=
EW-11 Mk\qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ji[mlEPTB;ND6xN|I3OSEQvF2= NGHROFRUSU6JRWK=
KARPAS-299 M1\iSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33YO2lEPTB;ND6zNVAyOyEQvF2= M4WzUnNCVkeHUh?=
SNU-C2B M2LHW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonKTWM2OD12LkO0Olg3KM7:TR?= NE\zVI9USU6JRWK=
LU-139 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXwTWM2OD12LkO3NlQ4KM7:TR?= MlvjV2FPT0WU
HCC1937 Mkj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\t[oZKSzVyPUSuN|c6QTNizszN NY\tNHlRW0GQR1XS
GR-ST M2fFc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1e1S2lEPTB;ND60N|E5KM7:TR?= M33iXnNCVkeHUh?=
SK-OV-3 NF\uZ3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2f0TGlEPTB;ND60OlA1OiEQvF2= MVnTRW5ITVJ?
A172 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zwcWlEPTB;ND61OVUzOSEQvF2= NUnsNIdKW0GQR1XS
NB10 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7TTotKSzVyPUSuOVk4QDFizszN NFTwTmlUSU6JRWK=
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KYSE-520 M{jJNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTRwOEiwNVgh|ryP MVXTRW5ITVJ?
KNS-62 MnruS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TscWlEPTB;ND65NFgxPyEQvF2= MXHTRW5ITVJ?
ES1 NXPYWlBTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTRwOU[wN|Uh|ryP NYXCNlhnW0GQR1XS
KOSC-2 M1vhOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTRwOUm5OVkh|ryP M4DBXnNCVkeHUh?=
TE-9 M4f5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjxcG9XUUN3ME21MlAyPTJ3IN88US=> Mnn2V2FPT0WU
NCI-H1838 NUn2TZpGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzSRZpKSzVyPUWuNFIxPzZizszN MkntV2FPT0WU
KP-N-YN NVnPWVdxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUm2VGtEUUN3ME21MlEzPTlzIN88US=> Mn\nV2FPT0WU
ALL-PO M3PYbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfWOFBnUUN3ME21MlIzPTh5IN88US=> Mnv2V2FPT0WU
NOMO-1 M37IWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml6yTWM2OD13LkKzNVc3KM7:TR?= Mn3JV2FPT0WU
CGTH-W-1 NYjnTGFyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTVwM{i1PFQh|ryP MVnTRW5ITVJ?
ATN-1 NX3DNmI2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTVwNEGxPFQh|ryP NHm5PYlUSU6JRWK=
AGS M3TzRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfyTWM2OD13LkS5OlE5KM7:TR?= NUfWXHJ[W0GQR1XS
SW13 Mmq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTQTWM2OD13LkWzNlY2KM7:TR?= NVPSNmdKW0GQR1XS
SW1990 M1Lyb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvGOHdKSzVyPUWuOlIzQDVizszN M1PJfHNCVkeHUh?=
BFTC-905 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLFNodXUUN3ME21MlY2QDd6IN88US=> NVXPZWt[W0GQR1XS
RMG-I MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;6TWM2OD13Lk[1PVQzKM7:TR?= MVvTRW5ITVJ?
HD-MY-Z MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXlTWM2OD13Lk[4OVgyKM7:TR?= M{jLS3NCVkeHUh?=
HCC1187 M{\PdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;4SZJKSzVyPUWuO|ExOzJizszN M3rOOHNCVkeHUh?=
HCC2998 NIjVR|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LTPWlEPTB;NT63NVg4PyEQvF2= NV;hTI16W0GQR1XS
RT-112 NITYZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTVwOUCyOFUh|ryP MVnTRW5ITVJ?
CAKI-1 Mke4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvFTWM2OD14LkCwPVg2KM7:TR?= MmjOV2FPT0WU
HDLM-2 MoC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW[1O3VZUUN3ME22MlAyPDh7IN88US=> NWXBcY52W0GQR1XS
TE-10 NIn5OoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUX1SVNvUUN3ME22MlA1QDN|IN88US=> NV;Qb3RbW0GQR1XS
KE-37 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3neZNKSzVyPU[uNFczPThizszN MnG5V2FPT0WU
RCM-1 M{PxZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2j3OWlEPTB;Nj6wO|cyQSEQvF2= Mn;mV2FPT0WU
Mewo M4PKc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXHTWM2OD14LkGxOFg4KM7:TR?= Mo[3V2FPT0WU
L-428 NUTOS3FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fKT2lEPTB;Nj6xPFA{PyEQvF2= M2q0d3NCVkeHUh?=
8-MG-BA NGnR[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInE[HRKSzVyPU[uNVk{PzlizszN Ml;YV2FPT0WU
SK-MEL-28 M3zWemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vtcGlEPTB;Nj6yNFk3PyEQvF2= MoS5V2FPT0WU
VA-ES-BJ M3G5[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\xUmlEPTB;Nj6zNFQ3OiEQvF2= M4HsZnNCVkeHUh?=
CAL-120 MlT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{KyUGlEPTB;Nj60OVE3OiEQvF2= MWfTRW5ITVJ?
GAMG MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDUTWM2OD14LkWwOlIh|ryP NHLtem1USU6JRWK=
MSTO-221H MknwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTZwNUGxO{DPxE1? M{P2dHNCVkeHUh?=
GCT NIjqOmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWSxXZdXUUN3ME22MlU{Ozh5IN88US=> M3fpcnNCVkeHUh?=
SH-4 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVOzVGk3UUN3ME22MlU5QDR2IN88US=> M3;2NXNCVkeHUh?=
Calu-3 NV7NOmQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfkfIhGUUN3ME22MlY2PTZ6IN88US=> MoG0V2FPT0WU
SK-HEP-1 MnHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlOzTWM2OD14LkexO|U{KM7:TR?= NWDJ[Y1rW0GQR1XS
PFSK-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17kXmlEPTB;Nj63N|Q4QCEQvF2= NGrrWXpUSU6JRWK=
NB12 MnK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3Lob2lEPTB;Nj63PFQxPCEQvF2= NYniZlFKW0GQR1XS
A388 NF[wcmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mni1TWM2OD14Lki5OVIh|ryP MXzTRW5ITVJ?
KLE NIDaOG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXQW4NLUUN3ME22Mlk3PTF4IN88US=> NUL6dY5mW0GQR1XS
TE-11 M2jXR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTdwMES5Olch|ryP MmT6V2FPT0WU
NCI-H2405 M1T3d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTdwMEizPVMh|ryP NH65[2lUSU6JRWK=
OVCAR-4 NEDTSllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTdwMUWzOUDPxE1? MVXTRW5ITVJ?
KP-N-YS NXrMb2x[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfkTWM2OD15LkG4NVQh|ryP M{G4S3NCVkeHUh?=
NOS-1 MlrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTdwMkW4Nlgh|ryP NUPHVnlDW0GQR1XS
DMS-114 NFPaV|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37U[mlEPTB;Nz6yPVY{OSEQvF2= NGDtcmFUSU6JRWK=
NCI-H2030 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHX3ZWVKSzVyPUeuN|M3QThizszN MVjTRW5ITVJ?
HEL NYn0SIwzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXjXIJKSzVyPUeuN|c2OjFizszN MmjqV2FPT0WU
CAL-62 M4noXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MleyTWM2OD15LkO5PFY3KM7:TR?= NWXnfnNXW0GQR1XS
COLO-668 NXniO4FWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfpTWM2OD15LkSzPVY4KM7:TR?= NWHqfFhmW0GQR1XS
SKG-IIIa NX;nW29PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvpcXlTUUN3ME23MlQ2OzhzIN88US=> NVTwVHQxW0GQR1XS
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T98G MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTN5Lki2N|Uh|ryP NInndZpUSU6JRWK=
NB14 M1PZXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGL0WndKSzVyPUO4MlQ2PDRizszN NUi3d29LW0GQR1XS
D-423MG NHflWIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTpWFZKSzVyPUO4MlQ5PjlizszN NHe1UINUSU6JRWK=
NCI-H510A NEjENXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE[1TndKSzVyPUO4MlY1PTFizszN M3\HfnNCVkeHUh?=
NMC-G1 MoLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml[xTWM2OD1|OD63NlIzKM7:TR?= MmrsV2FPT0WU
SW948 NWnONlc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTN6Lki5OFkh|ryP MYXTRW5ITVJ?
MFM-223 M2PIbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHFdXNKSzVyPUO4Mlk1OzFizszN NHTLNlZUSU6JRWK=
PAN1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\hTWM2OD1|OD65PVE6KM7:TR?= NES5cWhUSU6JRWK=
MZ7-mel NGrCTZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml[2TWM2OD1|OT6xNVk6KM7:TR?= M3voWXNCVkeHUh?=
COLO-679 NHWwTVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTN7Lkm3NFgh|ryP NF:4bWJUSU6JRWK=
SW872 M2\zNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXvOZd3UUN3ME20NE4zODNzIN88US=> Mm\pV2FPT0WU
HT-1197 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{W5XWlEPTB;NECuNlg3QSEQvF2= MV;TRW5ITVJ?
ES6 NEXEWWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PUdGlEPTB;NECuOFU2OiEQvF2= NETCUZpUSU6JRWK=
SNU-387 M4faXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGriN|RKSzVyPUSwMlgzOzhizszN M2OxVHNCVkeHUh?=
T47D MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTRzLkCxPVIh|ryP NETNeFRUSU6JRWK=
G-402 NFj0VFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TrXGlEPTB;NEGuNFgxQSEQvF2= NIXqRmFUSU6JRWK=
MZ1-PC M3:wXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1ntZ2lEPTB;NEGuPFEh|ryP MlW0V2FPT0WU
SW1417 NIDQdXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTR{LkOwN{DPxE1? NF\0Z4lUSU6JRWK=
RERF-LC-MS NXvD[3pkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnu1TWM2OD12Mj61NFg4KM7:TR?= MoO1V2FPT0WU
SF539 NXXFTnZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYm3cIR[UUN3ME20Nk42PTJ7IN88US=> NHPJW3dUSU6JRWK=
ESS-1 NUnDVHN{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\wTWM2OD12Mz61OVU5KM7:TR?= M3K1dXNCVkeHUh?=
RO82-W-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTR|Lk[xOVMh|ryP MWnTRW5ITVJ?
S-117 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LFT2lEPTB;NEOuOlk2QCEQvF2= MnnTV2FPT0WU
SW756 NG[zWHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfw[oRKSzVyPUSzMlgzPDJizszN M37KUnNCVkeHUh?=
CP66-MEL MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTR3LkexOFMh|ryP MWPTRW5ITVJ?
H-EMC-SS M3HjPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\4TWM2OD12NT63Olk{KM7:TR?= NY\LcpVGW0GQR1XS
NCI-H2052 NF:1dnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3yfZN2UUN3ME20OU46ODJ5IN88US=> MYXTRW5ITVJ?
SW684 NIL3eYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3qb3FKSzVyPUS3MlAxQDFizszN MULTRW5ITVJ?
HCC1569 NW\nPGxlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;XfFlKSzVyPUS3MlE3PThizszN M1v1T3NCVkeHUh?=
KGN MkfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1[3NWlEPTB;NEeuN|MyOSEQvF2= MWnTRW5ITVJ?
LCLC-103H MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW0dmljUUN3ME20O{41PDB2IN88US=> Mlu4V2FPT0WU
SW780 NUHre286T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\4bI1tUUN3ME20O{43OzVzIN88US=> NHHjZW1USU6JRWK=
NCI-H1703 Ml7uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nXOmlEPTB;NEiuN|M2OiEQvF2= NVe2S5p2W0GQR1XS
HCC1395 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHmzUJZKSzVyPUS4Mlc1OzJizszN MYjTRW5ITVJ?

多くの細胞株試験データを見る場合、クリックしてください

体内試験 Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

お薦めの試験操作(参考用のみ)

細胞試験: [1]
+ 展開
  • 細胞株: NR6, NR6M and NR6W cells
  • 濃度: 0-2 μM
  • 反応時間: 72 hours
  • 実験の流れ: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (参考用のみ)
動物試験:[3]
+ 展開
  • 動物モデル: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • 製剤: 0.5% polysorbate
  • 投薬量: 100 mg/kg
  • 投与方法: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 順序で溶剤を入れること:
1% CMC Na
30mg/mL

* 溶解度検測はSelleck技術部門によって行いますので、文献より提供された溶解度と差異がある可能性がありますが、生産工芸と不同ロット(lot)で起きる正常な現象ですから、ご安心ください。

化学情報

分子量 446.90
化学式

C22H24ClFN4O3

CAS No. 184475-35-2
保管
別名 N/A

便利ツール

モル濃度計算器

モル濃度計算器

解決のために必要とされるマス、ボリュームまたは濃度を計算してください。

マス (g) = 濃度 (mol/L) x ボリューム (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • マス
    濃度
    ボリューム
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備することを要求される希釈剤を計算してください. セレック希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 輸入 輸出 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

マス 濃度 ボリューム 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技術サポート

ストックの作り方、阻害剤の保管する方法、細胞実験や動物実験に注意すべきな点を全部含めており、製品を取扱う時よくあった質問に対して取扱説明書でお答えいたします。

Handling Instructions

他の質問がある場合は、お気軽くお問合せください。

  • * 必須

よくある質問(FAQ)

  • 問題1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

EGFR信号経路図

EGFR Inhibitors with Unique Features

相関EGFR製品

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