GPX1/2 Antibody [L20K22]

Catalog No.: F4438

Application: Reactivity: Human, Mouse, Rat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:100-1:1000 IHC1:50-1:500 IF1:50-1:500

Application
WB, IP, IHC, IF, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
23 kDa, 92 kDa

Datasheet & SDS

生物学的記述

Specificity
GPX1/2 Antibody [L20K22] detects endogenous levels of total GPX1/2 protein.

Clone
L20K22

Synonym(s)
Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; Phospholipid-hydroperoxide glutathione peroxidase GPX1; GPX1; Glutathione peroxidase 2; GPx-2; GSHPx-2; Gastrointestinal glutathione peroxidase; GPX2

Background
Glutathione peroxidases GPx1 and GPx2 are essential antioxidant enzymes that protect cells from oxidative damage by catalyzing the reduction of hydrogen peroxide and lipid hydroperoxides to water and corresponding alcohols, thereby preventing lipid peroxidation of cell membranes. Both enzymes contain a selenocysteine residue at their active site, encoded by the UGA stop codon, which is critical for their catalytic activity. GPx1 is ubiquitously expressed and is particularly important for protecting vascular and neural cells from oxidative stress, while GPx2 is mainly found in the gastrointestinal tract and lungs, with its expression regulated by the Nrf2 transcription factor in response to oxidative stress. GPx1 and GPx2 function as homodimers composed of alpha-helices and beta-sheets. Their catalytic mechanism involves the oxidation of the selenol group of selenocysteine by peroxides, followed by reduction by glutathione, which restores the active enzyme. Dysfunction of GPx1 or GPx2 is associated with increased risk of cardiovascular diseases, neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease, cancer, and heavy metal toxicity.

Background

Glutathione peroxidases GPx1 and GPx2 are essential antioxidant enzymes that protect cells from oxidative damage by catalyzing the reduction of hydrogen peroxide and lipid hydroperoxides to water and corresponding alcohols, thereby preventing lipid peroxidation of cell membranes. Both enzymes contain a selenocysteine residue at their active site, encoded by the UGA stop codon, which is critical for their catalytic activity. GPx1 is ubiquitously expressed and is particularly important for protecting vascular and neural cells from oxidative stress, while GPx2 is mainly found in the gastrointestinal tract and lungs, with its expression regulated by the Nrf2 transcription factor in response to oxidative stress. GPx1 and GPx2 function as homodimers composed of alpha-helices and beta-sheets. Their catalytic mechanism involves the oxidation of the selenol group of selenocysteine by peroxides, followed by reduction by glutathione, which restores the active enzyme. Dysfunction of GPx1 or GPx2 is associated with increased risk of cardiovascular diseases, neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease, cancer, and heavy metal toxicity.

References
[1] Lubos E, et al. Antioxid Redox Signal. 2011 Oct 1;15(7):1957-97. [2] Handy DE, et al. Free Radic Biol Med. 2022 Aug 1;188:146-161.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%