Human COMMD1 Antibody [B20N14]

Catalog No.: F4539

Application: Reactivity: Human

Lane 1: U2OS, Lane 2: HepG2

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:5000 IF1:40-1:125

Application
WB, IF

Source
Mouse Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
21 kDa

Datasheet & SDS

生物学的記述

Specificity
Human COMMD1 Antibody [B20N14] detects endogenous levels of total COMMD1 protein.

Clone
B20N14

Synonym(s)
C2orf5; COMM domain-containing protein 1; COMMD1; copper metabolism (Murr1) domain containing 1; copper metabolism gene MURR1; MGC27155; MURR1; MURR1chromosome 2 open reading frame 5 (MURR1); Protein Murr1

Background
COMMD1, also known as MURR1 and the copper toxicosis gene product, is a ubiquitously expressed 21 kDa copper-binding protein that plays versatile roles in cellular homeostasis and protein regulation. COMMD1 consists of an N-terminal α-helical VARiable Domain (VARD) spanning amino acids 1-121 and a C-terminal domain containing the COMM motif (amino acids 125-190), separated by a protease-sensitive site; this arrangement facilitates oligomerization into high molecular weight complexes both in solution and when associated with phospholipid membranes. COMMD1 localizes to the nucleus, cytoplasm, and endocytic vesicle membranes, where it interacts with multiple key proteins, including the copper transporter ATP7B (implicated in Wilson disease), the delta subunit of the epithelial sodium channel (ENaC), cystic fibrosis transmembrane conductance regulator (CFTR), superoxide dismutase 1 (SOD1), XIAP, HIF-1α, and Cullin-RING ubiquitin ligases. COMMD1 regulates protein folding, stability, ubiquitination, and proteolysis, notably promoting ubiquitination and degradation of the NF-κB RelA subunit, thereby inhibiting its nuclear translocation and activity; importantly, COMMD1 also restricts HIV replication in resting T cells. Its dysregulation is linked to neurodegenerative diseases, cancer progression, and disorders of copper metabolism.

Background

COMMD1, also known as MURR1 and the copper toxicosis gene product, is a ubiquitously expressed 21 kDa copper-binding protein that plays versatile roles in cellular homeostasis and protein regulation. COMMD1 consists of an N-terminal α-helical VARiable Domain (VARD) spanning amino acids 1-121 and a C-terminal domain containing the COMM motif (amino acids 125-190), separated by a protease-sensitive site; this arrangement facilitates oligomerization into high molecular weight complexes both in solution and when associated with phospholipid membranes. COMMD1 localizes to the nucleus, cytoplasm, and endocytic vesicle membranes, where it interacts with multiple key proteins, including the copper transporter ATP7B (implicated in Wilson disease), the delta subunit of the epithelial sodium channel (ENaC), cystic fibrosis transmembrane conductance regulator (CFTR), superoxide dismutase 1 (SOD1), XIAP, HIF-1α, and Cullin-RING ubiquitin ligases. COMMD1 regulates protein folding, stability, ubiquitination, and proteolysis, notably promoting ubiquitination and degradation of the NF-κB RelA subunit, thereby inhibiting its nuclear translocation and activity; importantly, COMMD1 also restricts HIV replication in resting T cells. Its dysregulation is linked to neurodegenerative diseases, cancer progression, and disorders of copper metabolism.

References
[1] Weiskirchen R, et al. J Trace Elem Med Biol. 2021 May;65:126712. [2] Vonk WI, et al. PLoS One. 2014 Apr 1;9(4):e92408.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%