Human Nuclear Antigen Antibody [M1M8]

Catalog No.: F1158

Application: Reactivity: Human, Primates

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:400-1:800 IF1:200-1:400 FCM1:200-1:400

Application
IHC, IF, FCM

Source
Mouse Monoclonal Antibody

Reactivity
Human, Primates

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
Human Nuclear Antigen Antibody [M1M8] detects endogenous levels of total Human Nuclear Antigen protein.

Clone
M1M8

Synonym(s)
HNA; HuNu

Background
Human Nuclear Antigen, commonly referred to as Proliferating Cell Nuclear Antigen (PCNA), is a conserved homotrimeric protein that functions as a DNA sliding clamp essential for DNA replication and repair. It forms a ring-shaped complex comprising three identical subunits, each containing two similar domains linked by an interdomain-bridging loop, which creates a central channel that encircles DNA and facilitates processive polymerase activity. The protein’s outer surface contains hydrophobic pockets that mediate interactions with various partner proteins through conserved PIP-box motifs, enabling its role as a critical coordinator in DNA synthesis, repair pathways, and cell cycle regulation. Post-translational modifications such as ubiquitination and SUMOylation dynamically regulate PCNA's interactions and functional switching, which is vital for maintaining genome stability. Aberrations in PCNA function or regulation have significant implications for cancer development, resulting in disrupted DNA replication fidelity and impaired repair mechanisms.

Background

Human Nuclear Antigen, commonly referred to as Proliferating Cell Nuclear Antigen (PCNA), is a conserved homotrimeric protein that functions as a DNA sliding clamp essential for DNA replication and repair. It forms a ring-shaped complex comprising three identical subunits, each containing two similar domains linked by an interdomain-bridging loop, which creates a central channel that encircles DNA and facilitates processive polymerase activity. The protein’s outer surface contains hydrophobic pockets that mediate interactions with various partner proteins through conserved PIP-box motifs, enabling its role as a critical coordinator in DNA synthesis, repair pathways, and cell cycle regulation. Post-translational modifications such as ubiquitination and SUMOylation dynamically regulate PCNA's interactions and functional switching, which is vital for maintaining genome stability. Aberrations in PCNA function or regulation have significant implications for cancer development, resulting in disrupted DNA replication fidelity and impaired repair mechanisms.

References
[1] González-Magaña A, et al. Biomolecules. 2020 Apr 8;10(4):570. [2] Kochaniak AB, et al. J Biol Chem. 2009 Jun 26;284(26):17700-10.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%