KCC2 Antibody [K20G10]

Catalog No.: F4340

Application: Reactivity: Human, Mouse, Rat, Zebrafish

Lane 1: Human brain, Lane 2: Mouse brain, Lane 3: Rat brain

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IHC1:500 IF1:1000

Application
WB, IHC, IF, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Zebrafish

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
140 kDa

Datasheet & SDS

生物学的記述

Specificity
KCC2 Antibody [K20G10] detects endogenous levels of total KCC2 protein.

Clone
K20G10

Synonym(s)
Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2 (rKCC2); Neuronal K-Cl cotransporter; Slc12a5; Kcc2

Background
KCC2 (potassium-chloride cotransporter 2, SLC12A5) is a neuron-specific cation-chloride cotransporter crucial for maintaining low intracellular chloride levels in mature central neurons. KCC2 contains twelve transmembrane domains forming a core transport region homologous to glutamate-GABA antiporters, with large intracellular N- and C-terminal domains; the C-terminus, comprising α/β subdomains, harbors multiple phosphorylation sites and mediates flexible dimerization via disulfide bridges, allowing KCC2 to exist as monomers or S-shaped dimers essential for stability and function. KCC2 operates as an electroneutral K+/Cl− extruder, utilizing the neuronal K+ gradient (established by Na+/K+-ATPase) to generate a hyperpolarizing chloride gradient, thereby ensuring that GABA_A and glycine receptor activation is inhibitory. Activity is tightly regulated by phosphorylation: WNK/SPAK kinases suppress KCC2 via C-terminal phosphorylation, while dephosphorylation activates it. KCC2 also modulates dendritic spine morphology, AMPA receptor content, and synaptic plasticity. Loss or dysfunction of KCC2 leads to intracellular Cl− accumulation and depolarizing GABA responses, contributing to epilepsy, autism spectrum disorders, schizophrenia, and neuropathic pain.

Background

KCC2 (potassium-chloride cotransporter 2, SLC12A5) is a neuron-specific cation-chloride cotransporter crucial for maintaining low intracellular chloride levels in mature central neurons. KCC2 contains twelve transmembrane domains forming a core transport region homologous to glutamate-GABA antiporters, with large intracellular N- and C-terminal domains; the C-terminus, comprising α/β subdomains, harbors multiple phosphorylation sites and mediates flexible dimerization via disulfide bridges, allowing KCC2 to exist as monomers or S-shaped dimers essential for stability and function. KCC2 operates as an electroneutral K+/Cl− extruder, utilizing the neuronal K+ gradient (established by Na+/K+-ATPase) to generate a hyperpolarizing chloride gradient, thereby ensuring that GABA_A and glycine receptor activation is inhibitory. Activity is tightly regulated by phosphorylation: WNK/SPAK kinases suppress KCC2 via C-terminal phosphorylation, while dephosphorylation activates it. KCC2 also modulates dendritic spine morphology, AMPA receptor content, and synaptic plasticity. Loss or dysfunction of KCC2 leads to intracellular Cl− accumulation and depolarizing GABA responses, contributing to epilepsy, autism spectrum disorders, schizophrenia, and neuropathic pain.

References
[1] Chamma I, Chevy Q, et al. Front Cell Neurosci. 2012 Feb 21;6:5. [2] Agez M, et al. Sci Rep. 2017 Nov 28;7(1):16452.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%