Lipin 1 Antibody [G22A22]

Catalog No.: F2643

Application: Reactivity: Human, Mouse

Lane 1: 3T3-L1, Lane 2: 3T3-L1 adipocytes (differentiated，7 d)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:100 IF1:1600

Application
WB, IP, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
130 kDa

Datasheet & SDS

生物学的記述

Specificity
Lipin 1 Antibody [G22A22] detects endogenous levels of total Lipin 1 protein.

Clone
G22A22

Synonym(s)
Phosphatidate phosphatase LPIN1; Lipin-1; LPIN1; KIAA0188

Background
Lipin 1 (LPIN1) belongs to the Lipin family of phosphatidate phosphatase enzymes (PAPs) that regulate lipid metabolism across multiple tissues, including adipose, liver, muscle, and brain. It features an N-terminal nuclear localization domain, a central catalytic Haloacid Dehalogenase (HAD)-like phosphatase domain responsible for Mg²⁺-dependent phosphatidic acid (PA) hydrolysis, and a C-terminal Lipin-specific region with a polybasic motif (K/R-rich sequence) that binds PA and serves as a nuclear localization signal. Key regulatory sites include phosphorylation motifs targeted by AKT1, mTOR, and LKB1, influencing its stability and subcellular shuttling between the nucleus and the endoplasmic reticulum. Lipin 1 exhibits dual roles: as a PAP enzyme, it converts PA to diacylglycerol (DAG) in the Kennedy pathway, driving triglyceride (TG), phosphatidylcholine, and phosphatidylethanolamine synthesis essential for lipid droplet formation and adipogenesis. As a transcriptional coactivator, it interacts with PPARα, PGC-1α, PPARγ, and C/EBPα in the nucleus to upregulate lipogenic and metabolic genes, while reciprocally enhancing PI3K-AKT-mTOR signaling via AKT1 activation. Lipin 1 mutations cause rhabdomyolysis, lipodystrophy with fatty liver, hypertriglyceridemia, insulin resistance, and are implicated in cancer via dysregulated mTOR pathways.

Background

Lipin 1 (LPIN1) belongs to the Lipin family of phosphatidate phosphatase enzymes (PAPs) that regulate lipid metabolism across multiple tissues, including adipose, liver, muscle, and brain. It features an N-terminal nuclear localization domain, a central catalytic Haloacid Dehalogenase (HAD)-like phosphatase domain responsible for Mg²⁺-dependent phosphatidic acid (PA) hydrolysis, and a C-terminal Lipin-specific region with a polybasic motif (K/R-rich sequence) that binds PA and serves as a nuclear localization signal. Key regulatory sites include phosphorylation motifs targeted by AKT1, mTOR, and LKB1, influencing its stability and subcellular shuttling between the nucleus and the endoplasmic reticulum. Lipin 1 exhibits dual roles: as a PAP enzyme, it converts PA to diacylglycerol (DAG) in the Kennedy pathway, driving triglyceride (TG), phosphatidylcholine, and phosphatidylethanolamine synthesis essential for lipid droplet formation and adipogenesis. As a transcriptional coactivator, it interacts with PPARα, PGC-1α, PPARγ, and C/EBPα in the nucleus to upregulate lipogenic and metabolic genes, while reciprocally enhancing PI3K-AKT-mTOR signaling via AKT1 activation. Lipin 1 mutations cause rhabdomyolysis, lipodystrophy with fatty liver, hypertriglyceridemia, insulin resistance, and are implicated in cancer via dysregulated mTOR pathways.

References
[1] Brohée L, et al. Int J Mol Sci. 2021 Apr 23;22(9):4419. [2] Un Nisa M, et al. J Cell Commun Signal. 2023 Sep;17(3):689-704.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%