NEDD8 Antibody [D22F6]

Catalog No.: F1133

Application: Reactivity: Mouse, Rat, Human

Lane 1: C6, Lane 2: Raw264.7, Lane 3: Hela, Lane 4: 3T3

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000 IP1:20-1:50 IHC1:100 - 1:500 IF1:100 - 1:250 FCM1:300

Application
WB, IP, IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
9 kDa 9 kDa, 12 kDa, 90 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
NEDD8 Antibody [D22F6] detects endogenous levels of total NEDD8 protein.

Clone
D22F6

Synonym(s)
Ubiquitin-like protein NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; NEDD8

Background
NEDD8 (neural precursor cell expressed developmentally downregulated protein 8) is a small ubiquitin-like protein (UBL) of the ubiquitin family, sharing about 60% sequence identity with ubiquitin and possessing a conserved ubiquitin-fold that is crucial for its conjugation activity. The mature NEDD8 protein consists of 76 amino acids, forming a globular β-grasp fold with an α-helix and four β-strands, a flexible C-terminal tail ending in Gly76 (which forms an isopeptide bond with lysine residues on target proteins), and surface residues that confer selectivity for NEDD8-activating enzymes. In a process called neddylation, which closely resembles ubiquitination, NEDD8 is activated by the E1 APP-BP1/Uba3 heterodimer, transferred to the E2 enzyme Ubc12, and then ligated by E3 ligases, mainly to cullin subunits of cullin-RING E3 ubiquitin ligases (CRLs). Neddylation of cullins triggers conformational changes that enhance E2-ubiquitin recruitment and substrate ubiquitination, thus promoting CRL activity. This modification regulates various cellular functions, including cell cycle progression (by degrading p27 and cyclin E), DNA damage responses, signal transduction (such as NF-κB and Wnt pathways), and protein homeostasis, but does not directly tag proteins for proteasomal degradation. Dysregulation of neddylation contributes to cancer (by stabilizing pro-proliferative proteins), neurodegeneration (via altered neuronal survival proteins), and other disorders like fibrosis.

Background

NEDD8 (neural precursor cell expressed developmentally downregulated protein 8) is a small ubiquitin-like protein (UBL) of the ubiquitin family, sharing about 60% sequence identity with ubiquitin and possessing a conserved ubiquitin-fold that is crucial for its conjugation activity. The mature NEDD8 protein consists of 76 amino acids, forming a globular β-grasp fold with an α-helix and four β-strands, a flexible C-terminal tail ending in Gly76 (which forms an isopeptide bond with lysine residues on target proteins), and surface residues that confer selectivity for NEDD8-activating enzymes. In a process called neddylation, which closely resembles ubiquitination, NEDD8 is activated by the E1 APP-BP1/Uba3 heterodimer, transferred to the E2 enzyme Ubc12, and then ligated by E3 ligases, mainly to cullin subunits of cullin-RING E3 ubiquitin ligases (CRLs). Neddylation of cullins triggers conformational changes that enhance E2-ubiquitin recruitment and substrate ubiquitination, thus promoting CRL activity. This modification regulates various cellular functions, including cell cycle progression (by degrading p27 and cyclin E), DNA damage responses, signal transduction (such as NF-κB and Wnt pathways), and protein homeostasis, but does not directly tag proteins for proteasomal degradation. Dysregulation of neddylation contributes to cancer (by stabilizing pro-proliferative proteins), neurodegeneration (via altered neuronal survival proteins), and other disorders like fibrosis.

References
[1] Shen LN, et al. EMBO J. 2005 Apr 6;24(7):1341-51. [2] Zhang S, et al. Signal Transduct Target Ther. 2024 Apr 5;9(1):85.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%