NF-YA Antibody [K24M4]

Catalog No.: F4432

Application: Reactivity: Human, Mouse, Rat

Lane 1: RAW264.7, Lane 2: NIH/3T3, Lane 3: 293T

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:200- 1:1,000 IHC1:50-1:500 IF1:50-1:500

Application
WB, IP, IHC, IF, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
30-50 kDa

Datasheet & SDS

生物学的記述

Specificity
NF-YA Antibody [K24M4] detects endogenous levels of total NF-YA protein.

Clone
K24M4

Synonym(s)
Nuclear transcription factor Y subunit alpha; CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A (NF-YA); NFYA

Background
NF-YA, a critical subunit of the heterotrimeric transcription factor Nuclear Factor Y (NF-Y), belongs to the family of sequence-specific DNA-binding proteins that recognize the CCAAT box motif found in the promoter regions of numerous eukaryotic genes. NF-YA partners with the NF-YB and NF-YC subunits, which together mimic histone proteins through a conserved histone-fold domain “handshake” motif, enabling the complex to bind DNA with high specificity and affinity. NF-YA contains two alpha helices essential for inserting into the DNA minor groove to provide sequence-specific recognition, whereas NF-YB and NF-YC primarily interact with the DNA backbone to stabilize binding. NF-Y serves as a pioneer factor, promoting chromatin accessibility, facilitating recruitment of transcription machinery, and regulating gene expression critical for cell growth, differentiation, and response to environmental stimuli. NF-YA’s sequence-specific DNA recognition also influences the transcription start site selection and modulates developmental processes including flowering in plants via regulation of FT gene expression.

Background

NF-YA, a critical subunit of the heterotrimeric transcription factor Nuclear Factor Y (NF-Y), belongs to the family of sequence-specific DNA-binding proteins that recognize the CCAAT box motif found in the promoter regions of numerous eukaryotic genes. NF-YA partners with the NF-YB and NF-YC subunits, which together mimic histone proteins through a conserved histone-fold domain “handshake” motif, enabling the complex to bind DNA with high specificity and affinity. NF-YA contains two alpha helices essential for inserting into the DNA minor groove to provide sequence-specific recognition, whereas NF-YB and NF-YC primarily interact with the DNA backbone to stabilize binding. NF-Y serves as a pioneer factor, promoting chromatin accessibility, facilitating recruitment of transcription machinery, and regulating gene expression critical for cell growth, differentiation, and response to environmental stimuli. NF-YA’s sequence-specific DNA recognition also influences the transcription start site selection and modulates developmental processes including flowering in plants via regulation of FT gene expression.

References
[1] Dolfini D, et al. Nucleic Acids Res. 2016 Jun 2;44(10):4684-702. [2] Zhao H, et al. Front Plant Sci. 2017 Jan 10;7:2045.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%