Phospho-Synapsin I (Ser553) Antibody [F18C24]

Catalog No.: F3953

Application: Reactivity: Mouse, Rat, Human

Lane 1: Mouse brain, Lane 2: Mouse brain (alkaline phosphatase treated), Lane 3: Rat brain

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:10000 - 1:50000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
74 kDa 77 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-Synapsin I (Ser553) Antibody [F18C24] detects endogenous levels of total -Synapsin I protein only when it is phosphorylated at Ser553.

Clone
F18C24

Synonym(s)
Synapsin-1; Brain protein 4.1; Synapsin I; SYN1

Background
Phospho-Synapsin I (Ser553) is a critical posttranslationally modified form of Synapsin I, a major neuronal phosphoprotein involved in the regulation of synaptic vesicle trafficking and neurotransmitter release in the central nervous system. Synapsin I consists of multiple domains, including an N-terminal domain that binds synaptic vesicles and a proline-rich C-terminal region containing Ser553, which is phosphorylated by proline-directed kinases such as MAP kinase and Cdk5-p23. This specific phosphorylation induces conformational changes that reduce Synapsin I’s affinity for synaptic vesicles, facilitating their mobilization from the reserve pool to the readily releasable pool, thereby promoting efficient neurotransmitter release, particularly of GABAergic vesicles. Phosphorylation at Ser553 is vital for synaptic plasticity, affecting processes underlying learning and memory by regulating vesicle dynamics and neurotransmission strength. Dysregulation of Ser553 phosphorylation has been linked to neurological disorders involving impaired synaptic transmission and cognitive deficits. The phosphorylation state of Synapsin I at Ser553 integrates extracellular signaling pathways with the cytoskeletal apparatus, modulating presynaptic function and neuronal communication.

Background

Phospho-Synapsin I (Ser553) is a critical posttranslationally modified form of Synapsin I, a major neuronal phosphoprotein involved in the regulation of synaptic vesicle trafficking and neurotransmitter release in the central nervous system. Synapsin I consists of multiple domains, including an N-terminal domain that binds synaptic vesicles and a proline-rich C-terminal region containing Ser553, which is phosphorylated by proline-directed kinases such as MAP kinase and Cdk5-p23. This specific phosphorylation induces conformational changes that reduce Synapsin I’s affinity for synaptic vesicles, facilitating their mobilization from the reserve pool to the readily releasable pool, thereby promoting efficient neurotransmitter release, particularly of GABAergic vesicles. Phosphorylation at Ser553 is vital for synaptic plasticity, affecting processes underlying learning and memory by regulating vesicle dynamics and neurotransmission strength. Dysregulation of Ser553 phosphorylation has been linked to neurological disorders involving impaired synaptic transmission and cognitive deficits. The phosphorylation state of Synapsin I at Ser553 integrates extracellular signaling pathways with the cytoskeletal apparatus, modulating presynaptic function and neuronal communication.

References
[1] Matsubara M, et al. J Biol Chem. 1996 Aug 30;271(35):21108-13. [2] Qiao S, et al. PLoS One. 2014 Apr 17;9(4):e95503.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%