UBQLN1 Antibody [M14H23]

Catalog No.: F9003

Application: Reactivity: Human, Monkey

Lane 1: OVCAR3, Lane 2: MCF-7, Lane 3: MOLT-4, Lane 4: COS-7

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
66 kDa

Datasheet & SDS

生物学的記述

Specificity
UBQLN1 Antibody [M14H23] detects endogenous levels of total UBQLN1 protein.

Clone
M14H23

Synonym(s)
Ubiquilin-1; Protein linking IAP with cytoskeleton 1 (PLIC-1; hPLIC-1); UBQLN1; DA41; PLIC1

Background
Ubiquilin-1 (UBQLN1) is a ubiquitously expressed member of the ubiquitin-like protein (UBL) family, characterized by an N-terminal UBL domain and a C-terminal ubiquitin-associated (UBA) domain that facilitates its role in protein quality control and degradation pathways. The UBL domain mediates interactions with proteasome subunits, while the UBA domain binds polyubiquitin chains, enabling UBQLN1 to shuttle ubiquitinated substrates to the proteasome for degradation. Additionally, UBQLN1 contains intrinsically disordered STI1 motifs implicated in client protein binding and involvement in proteostasis regulation. UBQLN1 is integral to the endoplasmic-reticulum-associated degradation (ERAD) pathway, transporting misfolded proteins from the ER to the proteasome, and also participates in aggresome formation by interacting with ubiquitin-interacting motif (UIM)-containing endocytic proteins. It regulates the turnover of presenilin proteins and is notably found in neurofibrillary tangles in Alzheimer’s disease, where polymorphisms in the UBQLN1 gene have been linked to increased disease risk.

Background

Ubiquilin-1 (UBQLN1) is a ubiquitously expressed member of the ubiquitin-like protein (UBL) family, characterized by an N-terminal UBL domain and a C-terminal ubiquitin-associated (UBA) domain that facilitates its role in protein quality control and degradation pathways. The UBL domain mediates interactions with proteasome subunits, while the UBA domain binds polyubiquitin chains, enabling UBQLN1 to shuttle ubiquitinated substrates to the proteasome for degradation. Additionally, UBQLN1 contains intrinsically disordered STI1 motifs implicated in client protein binding and involvement in proteostasis regulation. UBQLN1 is integral to the endoplasmic-reticulum-associated degradation (ERAD) pathway, transporting misfolded proteins from the ER to the proteasome, and also participates in aggresome formation by interacting with ubiquitin-interacting motif (UIM)-containing endocytic proteins. It regulates the turnover of presenilin proteins and is notably found in neurofibrillary tangles in Alzheimer’s disease, where polymorphisms in the UBQLN1 gene have been linked to increased disease risk.

References
[1] El Ayadi A, et al. Prion. 2013 Mar-Apr;7(2):164-9. [2] Zhang C, et al. Discov Med. 2009 Jun;8(40):18-22.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%