UQCRC2 Antibody [C21D5]

Catalog No.: F5996

Application: Reactivity: Human, Mouse, Rat

Lane 1: HepG2, Lane 2: Huh7, Lane 3: A172, Lane 4: 293T

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
48 kDa

Datasheet & SDS

生物学的記述

Specificity
UQCRC2 Antibody [C21D5] detects endogenous levels of total UQCRC2 protein.

Clone
C21D5

Synonym(s)
Cytochrome b-c1 complex subunit 2, mitochondrial; Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2; UQCRC2

Background
UQCRC2 (ubiquinol‑cytochrome c reductase core protein 2) is a nuclear‑encoded structural subunit of the mitochondrial cytochrome b‑c1 complex (Complex III) that is embedded in the inner mitochondrial membrane and contributes to the assembly, stability, and electron‑transport activity of the ubiquinol‑cytochrome c oxidoreductase complex. Within the electron transport chain, UQCRC2‑containing Complex III transfers electrons from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the inner membrane and supporting the electrochemical gradient that drives ATP synthesis and mitochondrial integrity. UQCRC2 expression is transcriptionally regulated by AMP‑activated protein kinase (AMPK) through activation of the transcription factor NFE2L2/NRF2, and this AMPK‑NRF2–UQCRC2 axis enhances UQCRC2 levels and promotes mitophagy, the selective clearance of damaged mitochondria, thereby protecting hepatocytes against alcohol‑induced liver injury. UQCRC2 is downregulated in tumor tissue compared with adjacent non‑carcinoma tissue, and its expression correlates inversely with lymph node metastasis, relapse, and tumor grade; UQCRC2 overexpression suppresses gastric cancer cell proliferation, migration, and invasion in vitro and in vivo, whereas reduction of UQCRC2 by upstream miRNAs such as miR‑370 enhances tumor aggressiveness.

Background

UQCRC2 (ubiquinol‑cytochrome c reductase core protein 2) is a nuclear‑encoded structural subunit of the mitochondrial cytochrome b‑c1 complex (Complex III) that is embedded in the inner mitochondrial membrane and contributes to the assembly, stability, and electron‑transport activity of the ubiquinol‑cytochrome c oxidoreductase complex. Within the electron transport chain, UQCRC2‑containing Complex III transfers electrons from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the inner membrane and supporting the electrochemical gradient that drives ATP synthesis and mitochondrial integrity. UQCRC2 expression is transcriptionally regulated by AMP‑activated protein kinase (AMPK) through activation of the transcription factor NFE2L2/NRF2, and this AMPK‑NRF2–UQCRC2 axis enhances UQCRC2 levels and promotes mitophagy, the selective clearance of damaged mitochondria, thereby protecting hepatocytes against alcohol‑induced liver injury. UQCRC2 is downregulated in tumor tissue compared with adjacent non‑carcinoma tissue, and its expression correlates inversely with lymph node metastasis, relapse, and tumor grade; UQCRC2 overexpression suppresses gastric cancer cell proliferation, migration, and invasion in vitro and in vivo, whereas reduction of UQCRC2 by upstream miRNAs such as miR‑370 enhances tumor aggressiveness.

References
[1] Lu X, et al. Autophagy. 2021 Nov;17(11):3622-3643. [2] Wang DW, et al. J Cancer. 2020 Jun 23;11(17):5042-5055.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%