VSV-G tag Antibody [N12B16]

Catalog No.: F2031

Application: Reactivity:

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IHC1:5000 IF1:2000 FCM1:500

Application
WB, IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Observed MW
100 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
VSV-G tag Antibody [N12B16] detects recombinant proteins containing the VSV-G epitope tag.

Clone
N12B16

Synonym(s)
VSV epitope tag; VSV tag; VSV-G epitope tag; VSVG; Vesicular stomatitis virus glycoprotein tag; vsvg tag

Background
The VSV-G tag is a short, highly immunogenic epitope (sequence: QMRTRRHLHV) derived from the membrane-proximal C-terminal region of vesicular stomatitis virus glycoprotein (VSV-G), a class III viral fusion protein belonging to the rhabdovirus glycoprotein family. The native VSV-G ectodomain folds into three conserved domains: a fusion domain (FD) with membrane-interacting loops (residues ~118-136), pleckstrin homology domain (PHD), and trimerization domain (TrD), connected by flexible hinge regions (R1-R5) that undergo pH-induced refolding; the tag corresponds to a linear, solvent-exposed antigenic site in the post-fusion conformation recognized by neutralizing antibodies like PIV. When fused to recombinant proteins, the small VSV-G tag (11 amino acids) minimally perturbs native folding and enables sensitive detection via commercial monoclonal antibodies in immunoblotting, immunoprecipitation, immunofluorescence, and flow cytometry without affecting subcellular localization or interactions. Native VSV-G mediates broad receptor binding (e.g., LDL-R, CR2/CR3 domains via basic residues H8, K47, R354), clathrin-mediated endocytosis, and low-pH-triggered fusion through irreversible trimer rearrangement, underpinning its use in pseudotyping lentiviral/retroviral vectors for stable, high-titer gene delivery across diverse cell types with no disease relevance as a tag.

Background

The VSV-G tag is a short, highly immunogenic epitope (sequence: QMRTRRHLHV) derived from the membrane-proximal C-terminal region of vesicular stomatitis virus glycoprotein (VSV-G), a class III viral fusion protein belonging to the rhabdovirus glycoprotein family. The native VSV-G ectodomain folds into three conserved domains: a fusion domain (FD) with membrane-interacting loops (residues ~118-136), pleckstrin homology domain (PHD), and trimerization domain (TrD), connected by flexible hinge regions (R1-R5) that undergo pH-induced refolding; the tag corresponds to a linear, solvent-exposed antigenic site in the post-fusion conformation recognized by neutralizing antibodies like PIV. When fused to recombinant proteins, the small VSV-G tag (11 amino acids) minimally perturbs native folding and enables sensitive detection via commercial monoclonal antibodies in immunoblotting, immunoprecipitation, immunofluorescence, and flow cytometry without affecting subcellular localization or interactions. Native VSV-G mediates broad receptor binding (e.g., LDL-R, CR2/CR3 domains via basic residues H8, K47, R354), clathrin-mediated endocytosis, and low-pH-triggered fusion through irreversible trimer rearrangement, underpinning its use in pseudotyping lentiviral/retroviral vectors for stable, high-titer gene delivery across diverse cell types with no disease relevance as a tag.

References
[1] Libersou S, et al. J Cell Biol. 2010 Oct 4;191(1):199-210. [2] Roche S, et al. Cell Mol Life Sci. 2008 Jun;65(11):1716-28.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%