5-hydroxymethylcytosine Antibody [B13H23]

Catalog No.: F2030

Application: Reactivity:

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:2000-1:20000 IF1:1000-1:4000 FCM1:400-1:2000

Application
IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
5-hydroxymethylcytosine Antibody [B13H23] detects endogenous levels of total 5-hydroxymethylcytosine protein.

Clone
B13H23

Synonym(s)
5-hmC

Background
5-hydroxymethylcytosine (5-hmC) is a distinct and significant epigenetic DNA modification resulting from the oxidation of 5-methylcytosine (5-mC) by the ten-eleven translocation (TET) family of dioxygenases (TET1, TET2, TET3). This modification adds a hydroxymethyl group at the 5-position of cytosine, altering the chemical landscape of DNA without disrupting base pairing, thereby influencing chromatin structure and gene regulation. 5-hmC differs from 5-mC by the presence of this hydroxymethyl group, which modulates the binding of methyl-CpG binding proteins (e.g., MeCP2) and recruits specialized reader proteins that mediate downstream effects. 5-hmC serves a dual role: as an intermediate in active DNA demethylation, where it undergoes further oxidation to 5-formylcytosine and 5-carboxylcytosine, followed by base excision repair, and as a stable epigenetic mark enriched in gene bodies, promoters, and enhancers, particularly in neuronal tissues. Its presence correlates with transcriptional activation by facilitating an open chromatin state and is dynamically regulated during development and in response to environmental cues. Aberrant 5-hmC patterns are implicated in tumorigenesis (e.g., gliomas) and neurological disorders such as Rett syndrome.

Background

5-hydroxymethylcytosine (5-hmC) is a distinct and significant epigenetic DNA modification resulting from the oxidation of 5-methylcytosine (5-mC) by the ten-eleven translocation (TET) family of dioxygenases (TET1, TET2, TET3). This modification adds a hydroxymethyl group at the 5-position of cytosine, altering the chemical landscape of DNA without disrupting base pairing, thereby influencing chromatin structure and gene regulation. 5-hmC differs from 5-mC by the presence of this hydroxymethyl group, which modulates the binding of methyl-CpG binding proteins (e.g., MeCP2) and recruits specialized reader proteins that mediate downstream effects. 5-hmC serves a dual role: as an intermediate in active DNA demethylation, where it undergoes further oxidation to 5-formylcytosine and 5-carboxylcytosine, followed by base excision repair, and as a stable epigenetic mark enriched in gene bodies, promoters, and enhancers, particularly in neuronal tissues. Its presence correlates with transcriptional activation by facilitating an open chromatin state and is dynamically regulated during development and in response to environmental cues. Aberrant 5-hmC patterns are implicated in tumorigenesis (e.g., gliomas) and neurological disorders such as Rett syndrome.

References
[1] López V, et al. Ageing Res Rev. 2017 Aug;37:28-38. [2] Shi DQ, et al. Front Genet. 2017 Jul 19;8:100.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%