Acetyl-Histone H4 (Lys16) Antibody [G21H2]

Catalog No.: F4674

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: HeLa, Lane 2: HeLa (TSA, 1 μM, 18 h), Lane 3: C2C12, Lane 4: C2C12 (TSA, 1 μM, 18 h)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50 IHC1:50 IF1:1600 FCM1:1600 CHIP1:50

Application
WB, IP, IHC, IF, FCM, ChIP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
11 kDa

Datasheet & SDS

生物学的記述

Specificity
Acetyl-Histone H4 (Lys16) Antibody [G21H2] detects endogenous levels of total Histone H4 protein only when it is Acetylated at Lys16.

Clone
G21H2

Synonym(s)
H4C1; H4/A; H4FA; HIST1H4A; H4C2; H4/I; H4FI; HIST1H4B; H4C3; H4/G; H4FG; HIST1H4C; H4C4; H4/B; H4FB; HIST1H4D; H4C5; H4/J; H4FJ; HIST1H4E; H4C6; H4/C; H4FC; HIST1H4F; H4C8; H4/H; H4FH; HIST1H4H; H4C9; H4/M; H4FM; HIST1H4I; H4C11; H4/E; H4FE

Background
Acetyl-Histone H4 (Lys16) is an epigenetic modification on the N-terminal tail of histone H4 that neutralizes the positive charge of lysine 16 through acetyl transfer catalyzed by HAT1 and MOF. This modification disrupts the electrostatic interaction between H4K16 and the H2A acidic patch, preventing chromatin fiber compaction and maintaining an open euchromatin conformation favorable for transcriptional activation. The acetylation of H4K16 creates a bromodomain docking site that recruits the SAS chromatin assembly complex and antagonists of Sir proteins at telomeres, helping to establish boundaries between heterochromatin and euchromatin. H4K16ac inhibits ACF remodeling enzyme activity on compacted chromatin fibers and uniquely abolishes H4 tail-mediated internucleosomal interactions compared to other acetylation sites. H4K16ac regulates gene expression, DNA repair, and chromatin remodeling by facilitating transcription factor and promoter access and by preventing the spread of Sir2/3/4 silencing complexes into euchromatic regions. H4K16ac marks accessible chromatin at sites of apoptosis-associated cleavage and regulates differentiation programs in neutrophils. Hyperacetylation of H4K16 is associated with embryonic lethality and male sterility in Sas2 mutants and contributes to cancer progression by maintaining sustained euchromatin domains.

Background

Acetyl-Histone H4 (Lys16) is an epigenetic modification on the N-terminal tail of histone H4 that neutralizes the positive charge of lysine 16 through acetyl transfer catalyzed by HAT1 and MOF. This modification disrupts the electrostatic interaction between H4K16 and the H2A acidic patch, preventing chromatin fiber compaction and maintaining an open euchromatin conformation favorable for transcriptional activation. The acetylation of H4K16 creates a bromodomain docking site that recruits the SAS chromatin assembly complex and antagonists of Sir proteins at telomeres, helping to establish boundaries between heterochromatin and euchromatin. H4K16ac inhibits ACF remodeling enzyme activity on compacted chromatin fibers and uniquely abolishes H4 tail-mediated internucleosomal interactions compared to other acetylation sites. H4K16ac regulates gene expression, DNA repair, and chromatin remodeling by facilitating transcription factor and promoter access and by preventing the spread of Sir2/3/4 silencing complexes into euchromatic regions. H4K16ac marks accessible chromatin at sites of apoptosis-associated cleavage and regulates differentiation programs in neutrophils. Hyperacetylation of H4K16 is associated with embryonic lethality and male sterility in Sas2 mutants and contributes to cancer progression by maintaining sustained euchromatin domains.

References
[1] Shia WJ, et al. Genome Biol. 2006;7(5):217. [2] Shogren-Knaak M, et al. Science. 2006 Feb 10;311(5762):844-7.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%