APC11 Antibody [G19D9]

Catalog No.: F9819

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: U2OS, Lane 3: H-4-II-E, Lane 4: COS-7

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:100

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
10 kDa

Datasheet & SDS

生物学的記述

Specificity
APC11 Antibody [G19D9] detects endogenous levels of total APC11 protein.

Clone
G19D9

Synonym(s)
Anaphase-promoting complex subunit 11; APC11; Cyclosome subunit 11; Hepatocellular carcinoma-associated RING finger protein; ANAPC11

Background
Anaphase‑promoting complex subunit 11 (APC11) is a small RING‑H2‑finger protein that forms part of the anaphase‑promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase essential for cell‑cycle progression from metaphase to anaphase through targeted degradation of key regulatory proteins by the 26S proteasome. APC11 contains a canonical RING‑H2 domain comprising non‑tandem histidine and cysteine residues that coordinate zinc ions and confer structural similarity to the RING domains of RBX1 and RBX2 in SCF‑type E3 ligases. Within the APC/C, APC11 heterodimerizes with the cullin‑like subunit APC2 to form the catalytic core, and this APC2–APC11 module functions as a ubiquitin‑acceptor scaffold that recruits ubiquitin‑charged E2 enzymes such as UBE2S and UBE2C, thereby enabling the assembly of polyubiquitin chains on APC/C substrates. The activity of the APC/C complex depends on coactivators CDC20 and Cdh1 (FZR1), which recognize substrate proteins via D‑box and KEN‑box motifs and present them to the APC2–APC11–APC10 ubiquitylation site, ensuring timely turnover of cyclins, securin, and other cell‑cycle regulators. Mutations that disrupt the integrity of the APC11 RING‑H2 finger abolish ubiquitin‑chain formation and compromise APC/C‑dependent substrate degradation. APC11 is required for the metaphase‑to‑anaphase transition, chromosome segregation, and exit from mitosis, and it is studied as a core catalytic component of the APC/C and as a mechanistic node in cell‑cycle control and genome‑stability pathways.

Background

Anaphase‑promoting complex subunit 11 (APC11) is a small RING‑H2‑finger protein that forms part of the anaphase‑promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase essential for cell‑cycle progression from metaphase to anaphase through targeted degradation of key regulatory proteins by the 26S proteasome. APC11 contains a canonical RING‑H2 domain comprising non‑tandem histidine and cysteine residues that coordinate zinc ions and confer structural similarity to the RING domains of RBX1 and RBX2 in SCF‑type E3 ligases. Within the APC/C, APC11 heterodimerizes with the cullin‑like subunit APC2 to form the catalytic core, and this APC2–APC11 module functions as a ubiquitin‑acceptor scaffold that recruits ubiquitin‑charged E2 enzymes such as UBE2S and UBE2C, thereby enabling the assembly of polyubiquitin chains on APC/C substrates. The activity of the APC/C complex depends on coactivators CDC20 and Cdh1 (FZR1), which recognize substrate proteins via D‑box and KEN‑box motifs and present them to the APC2–APC11–APC10 ubiquitylation site, ensuring timely turnover of cyclins, securin, and other cell‑cycle regulators. Mutations that disrupt the integrity of the APC11 RING‑H2 finger abolish ubiquitin‑chain formation and compromise APC/C‑dependent substrate degradation. APC11 is required for the metaphase‑to‑anaphase transition, chromosome segregation, and exit from mitosis, and it is studied as a core catalytic component of the APC/C and as a mechanistic node in cell‑cycle control and genome‑stability pathways.

References
[1] Schwedersky RP, et al. Front Plant Sci. 2021 Nov 23;12:563760. [2] Gmachl M, et al. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8973-8.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%