APG5L/ATG5 Antibody [K12K17]

Catalog No.: F2856

Application: Reactivity: Mouse, Rat, Human

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000 IP1:20 IHC1:100 - 1:250 IF1:100 - 1:250

Application
WB, IP, IHC, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
102 kDa,32 kDa 32 kDa, 55 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
APG5L/ATG5 Antibody [K12K17] detects endogenous levels of total APG5L/ATG5 protein.

Clone
K12K17

Synonym(s)
APG5L; ASP; ATG5; Autophagy protein 5; APG5-like; Apoptosis-specific protein

Background
ATG5 (Autophagy-related protein 5, also known as APG5L) is a core component of the autophagy machinery essential for autophagosome formation through ubiquitin-like conjugation systems in eukaryotic cells. ATG5 comprises an N-terminal ubiquitin-like domain (UblA), a central helix-rich domain (HR), and a C-terminal ubiquitin-like domain (UblB), connected by two flexible linker regions (L1 and L2), with an α-helix at the N-terminus where lysine residue K130 serves as the conjugation site for ATG12. ATG5 is activated by ATG7 (E1-like enzyme) and conjugated to ATG12 by ATG10 (E2-like enzyme), forming the ATG12-ATG5 conjugate that non-covalently binds ATG16L1 to create an E3-like ligase complex localized to the phagophore membrane. This complex facilitates LC3 lipidation (LC3-I to LC3-II conjugated to phosphatidylethanolamine), driving autophagosome elongation and maturation essential for degradation of damaged organelles and proteins during stress/starvation. ATG5 also exhibits autophagy-independent roles, including pro-apoptotic functions via mitochondrial translocation, cell cycle arrest through CDK1/CHEK2 phosphorylation, and immune regulation. Its dysregulation contributes to neurodegeneration, cancer, and autoinflammatory diseases.

Background

ATG5 (Autophagy-related protein 5, also known as APG5L) is a core component of the autophagy machinery essential for autophagosome formation through ubiquitin-like conjugation systems in eukaryotic cells. ATG5 comprises an N-terminal ubiquitin-like domain (UblA), a central helix-rich domain (HR), and a C-terminal ubiquitin-like domain (UblB), connected by two flexible linker regions (L1 and L2), with an α-helix at the N-terminus where lysine residue K130 serves as the conjugation site for ATG12. ATG5 is activated by ATG7 (E1-like enzyme) and conjugated to ATG12 by ATG10 (E2-like enzyme), forming the ATG12-ATG5 conjugate that non-covalently binds ATG16L1 to create an E3-like ligase complex localized to the phagophore membrane. This complex facilitates LC3 lipidation (LC3-I to LC3-II conjugated to phosphatidylethanolamine), driving autophagosome elongation and maturation essential for degradation of damaged organelles and proteins during stress/starvation. ATG5 also exhibits autophagy-independent roles, including pro-apoptotic functions via mitochondrial translocation, cell cycle arrest through CDK1/CHEK2 phosphorylation, and immune regulation. Its dysregulation contributes to neurodegeneration, cancer, and autoinflammatory diseases.

References
[1] Otomo C, et al. Nat Struct Mol Biol. 2013 Jan;20(1):59-66. [2] Ye X, et al. Front Immunol. 2018 Oct 17;9:2334.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%