Aquaporin 5 Antibody [N15B11]

Catalog No.: F1149

Application: Reactivity: Human

Lane 1: SW480 (RIPA lysis), Lane 2: SW480 (1%SDS Hot lysis)

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:10000 IHC1:1200

Application
WB, IHC

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
28 kDa 24 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Aquaporin 5 Antibody [N15B11] detects endogenous levels of total Aquaporin 5 protein.

Clone
N15B11

Synonym(s)
Aquaporin-5; AQP-5; AQP5

Background
Aquaporin 5 (AQP5) is a crucial member of the aquaporin family of water channel proteins, predominantly expressed in epithelial cells of secretory tissues such as salivary, lacrimal, and sweat glands, as well as in the lungs and eye. AQP5 functions as a homotetramer, with each of its four identical monomers comprising six transmembrane α-helices and two signature Asparagine-Proline-Alanine (NPA) motifs that are essential for selective water permeability while excluding protons and larger solutes. The selectivity filter, formed by the aromatic/arginine (ar/R) constriction region, ensures high water specificity and prevents ion leakage. AQP5 mediate rapid, osmotically driven water transport across epithelial membranes, which is vital for the secretion of fluids like saliva, tears, and airway surface liquid, thus maintaining tissue hydration and homeostasis. AQP5’s activity is tightly regulated by its trafficking to and from the plasma membrane, a process modulated by intracellular calcium and cAMP signaling, as well as interactions with regulatory proteins like ezrin, which are essential for its apical localization. Dysregulation of AQP5 is implicated in several diseases: in Sjögren’s syndrome, inflammatory mislocalization of AQP5 leads to impaired saliva and tear secretion, whereas in various cancers (including breast, lung, and colon), AQP5 overexpression enhances cell proliferation, migration, and metastasis through activation of pathways like Ras-MAPK.

Background

Aquaporin 5 (AQP5) is a crucial member of the aquaporin family of water channel proteins, predominantly expressed in epithelial cells of secretory tissues such as salivary, lacrimal, and sweat glands, as well as in the lungs and eye. AQP5 functions as a homotetramer, with each of its four identical monomers comprising six transmembrane α-helices and two signature Asparagine-Proline-Alanine (NPA) motifs that are essential for selective water permeability while excluding protons and larger solutes. The selectivity filter, formed by the aromatic/arginine (ar/R) constriction region, ensures high water specificity and prevents ion leakage. AQP5 mediate rapid, osmotically driven water transport across epithelial membranes, which is vital for the secretion of fluids like saliva, tears, and airway surface liquid, thus maintaining tissue hydration and homeostasis. AQP5’s activity is tightly regulated by its trafficking to and from the plasma membrane, a process modulated by intracellular calcium and cAMP signaling, as well as interactions with regulatory proteins like ezrin, which are essential for its apical localization. Dysregulation of AQP5 is implicated in several diseases: in Sjögren’s syndrome, inflammatory mislocalization of AQP5 leads to impaired saliva and tear secretion, whereas in various cancers (including breast, lung, and colon), AQP5 overexpression enhances cell proliferation, migration, and metastasis through activation of pathways like Ras-MAPK.

References
[1] Direito I, et al. Cell Mol Life Sci. 2016 Apr;73(8):1623-40. [2] Horsefield R, et al. Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13327-32.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%