ホームページ 一次抗体 ATP-binding cassette sub-family A member 3 Antibody [F19C21] For research use only.
Not for use in humans.

ATP-binding cassette sub-family A member 3 Antibody [F19C21]

Catalog No.: F2545

    Application: Reactivity:
    • Immunohistochemical analysis of formalin fixed paraffin embedded mouse lung tissue with F2545 at 1:100 dilution.
    1/

    当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

    代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

    使用情報

    Dilution
    1:10
    Application
    IHC
    Source
    Mouse Monoclonal Antibody
    Reactivity
    Mouse, Rat
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    ポジティブコントロール Mouse lung; Rat lung
    ネガティブコントロール

    プロトコール

    IHC
    Experimental Protocol：
     
    Deparaffinization/Rehydration
    1. Deparaffinize/hydrate sections:
    2. Incubate sections in three washes of xylene for 5 min each.
    3. Incubate sections in two washes of 100% ethanol for 10 min each.
    4. Incubate sections in two washes of 95% ethanol for 10 min each.
    5. Wash sections two times in dH2O for 5 min each.
    6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
     
    Staining
    1. Wash sections in dH2O three times for 5 min each.
    2. Incubate sections in 3% hydrogen peroxide for 10 min.
    3. Wash sections in dH2O two times for 5 min each.
    4. Wash sections in wash buffer for 5 min.
    5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature.
    6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C.
    7. Remove antibody solution and wash sections with wash buffer three times for 5 min each.
    8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature.
    9. Wash sections three times with wash buffer for 5 min each.
    10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use.
    11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.
    12. Immerse slides in dH2O.
    13. If desired, counterstain sections with hematoxylin.
    14. Wash sections in dH2O two times for 5 min each.
    15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each.
    16. Mount sections with coverslips and mounting medium.
     

    Datasheet & SDS

    生物学的記述

    Specificity

    ATP-binding cassette sub-family A member 3 Antibody [F19C21] detects endogenous levels of total ATP-binding cassette sub-family A member 3 protein.
    タンパク質の局在
    細胞質小胞、エンドソーム、リソソーム、細胞内膜系
    Uniprot ID
    Q99758
    Clone
    F19C21
    Synonym(s)
    Phospholipid-transporting ATPase ABCA3, ATP-binding cassette sub-family A member 3, Xenobiotic-transporting ATPase ABCA3, Abca3
    Background
    ATP-binding cassette sub-family A member 3 (ABCA3) is a highly conserved, multi-pass transmembrane glycoprotein in the ABC transporter superfamily that uses ATP hydrolysis to transport phospholipids into lamellar bodies (LBs) of alveolar type II cells, where pulmonary surfactant is assembled and stored. Structurally, ABCA3 consists of two similar halves, each containing six transmembrane domains and a cytosolic nucleotide-binding domain (NBD) with Walker A, Walker B, and ABCA-specific signature motifs, plus two essential N-linked glycosylation sites at Asn124 and Asn140 required for proper folding, stability, and LB targeting. Although expressed in multiple tissues, ABCA3 is highly enriched in the lung, where it is critical for LB biogenesis and surfactant homeostasis, particularly in transporting phosphatidylcholine, phosphatidylglycerol, and cholesterol. Mutations in ABCA3 can cause trafficking or functional defects, leading to neonatal surfactant deficiency, interstitial lung disease, and fatal respiratory distress.
    References

    技術サポート

    ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

    Handling Instructions

    他に質問がある場合は、お気軽にお問い合わせください。

    * 必須

    大学・企業名を記入してください
    名前を記入してください
    電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
    お問い合わせ内容をご入力ください
    united states