BRD4 Antibody [E1J10]

Catalog No.: F4202

Application: Reactivity: Human

Lane 1: 293T, Lane 2: Jurkat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50 IHC1:20000 - 1:80000 CHIP1:50

Application
WB, IP, IHC, ChIP

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
200 kDa

Datasheet & SDS

生物学的記述

Specificity
BRD4 (E2A7X) Rabbit mAb detects endogenous levels of total BRD4 protein.

Clone
E1J10

Synonym(s)
Bromodomain-containing protein 4; Protein HUNK1; BRD4

Background
Bromodomain-containing protein 4 (BRD4) is a member of the BET (bromodomains and extra-terminal) family of chromatin reader proteins, which also includes BRD2, BRD3, and BRDT, and is defined by the presence of two tandem bromodomains (BD1 and BD2) and an ET domain. BRD4 contains an N-terminal region with BD1 and BD2, each consisting of four α-helices connected by ZA and BC loops that specifically recognize acetylated lysines on histones H3 and H4, as well as conserved motifs for DNA binding, a basic interaction domain, a three-helix ET domain (amino acids 600–678), a SEED domain, and a C-terminal motif (CTM, aa 1325–1362) for mediator complex interactions. BRD4 binds acetylated chromatin throughout the cell cycle, including mitosis, where it acts as a bookmark for rapid gene reactivation after cell division. It recruits the pTEFb complex through BD2 and CTM to phosphorylate Ser2 on the CTD of RNA polymerase II, promoting transcription elongation, and scaffolds the super-elongation and PAFc complexes, particularly in MLL-fusion leukemias. BRD4 regulates inflammatory gene expression (e.g., via NF-κB/RelA), Myc-driven oncogenes, and survival genes like Bcl-2 and CDK6 in cancers such as Burkitt's lymphoma and NUT midline carcinoma, where BET inhibitors can displace BRD4 from chromatin to induce apoptosis and exert antitumor effects.

Background

Bromodomain-containing protein 4 (BRD4) is a member of the BET (bromodomains and extra-terminal) family of chromatin reader proteins, which also includes BRD2, BRD3, and BRDT, and is defined by the presence of two tandem bromodomains (BD1 and BD2) and an ET domain. BRD4 contains an N-terminal region with BD1 and BD2, each consisting of four α-helices connected by ZA and BC loops that specifically recognize acetylated lysines on histones H3 and H4, as well as conserved motifs for DNA binding, a basic interaction domain, a three-helix ET domain (amino acids 600–678), a SEED domain, and a C-terminal motif (CTM, aa 1325–1362) for mediator complex interactions. BRD4 binds acetylated chromatin throughout the cell cycle, including mitosis, where it acts as a bookmark for rapid gene reactivation after cell division. It recruits the pTEFb complex through BD2 and CTM to phosphorylate Ser2 on the CTD of RNA polymerase II, promoting transcription elongation, and scaffolds the super-elongation and PAFc complexes, particularly in MLL-fusion leukemias. BRD4 regulates inflammatory gene expression (e.g., via NF-κB/RelA), Myc-driven oncogenes, and survival genes like Bcl-2 and CDK6 in cancers such as Burkitt's lymphoma and NUT midline carcinoma, where BET inhibitors can displace BRD4 from chromatin to induce apoptosis and exert antitumor effects.

References
[1] McBride AA, et al. Viruses. 2013 May 30;5(6):1374-94. [2] Devaiah BN, et al. J Leukoc Biol. 2016 Oct;100(4):679-686.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%