C1q Antibody [G8H3]

Catalog No.: F1660

Application: Reactivity: Mouse

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain tissue with F1660 at 1:1000 dilution. ,
Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain tissue with F1660 at 1:1000 dilution.,

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:1000

Application
IHC

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

ポジティブコントロール	Mouse cerebral cortex
ネガティブコントロール

プロトコール

IHC
Experimental Protocol： Deparaffinization/Rehydration 1. Deparaffinize/hydrate sections: 2. Incubate sections in three washes of xylene for 5 min each. 3. Incubate sections in two washes of 100% ethanol for 10 min each. 4. Incubate sections in two washes of 95% ethanol for 10 min each. 5. Wash sections two times in dH2O for 5 min each. 6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min. Staining 1. Wash sections in dH2O three times for 5 min each. 2. Incubate sections in 3% hydrogen peroxide for 10 min. 3. Wash sections in dH2O two times for 5 min each. 4. Wash sections in wash buffer for 5 min. 5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature. 6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C. 7. Remove antibody solution and wash sections with wash buffer three times for 5 min each. 8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature. 9. Wash sections three times with wash buffer for 5 min each. 10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use. 11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity. 12. Immerse slides in dH2O. 13. If desired, counterstain sections with hematoxylin. 14. Wash sections in dH2O two times for 5 min each. 15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each. 16. Mount sections with coverslips and mounting medium.

IHC

Experimental Protocol：

Deparaffinization/Rehydration

1. Deparaffinize/hydrate sections:

2. Incubate sections in three washes of xylene for 5 min each.

3. Incubate sections in two washes of 100% ethanol for 10 min each.

4. Incubate sections in two washes of 95% ethanol for 10 min each.

5. Wash sections two times in dH2O for 5 min each.

6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.

Staining

1. Wash sections in dH2O three times for 5 min each.

2. Incubate sections in 3% hydrogen peroxide for 10 min.

3. Wash sections in dH2O two times for 5 min each.

4. Wash sections in wash buffer for 5 min.

5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature.

6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C.

7. Remove antibody solution and wash sections with wash buffer three times for 5 min each.

8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature.

9. Wash sections three times with wash buffer for 5 min each.

10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use.

11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.

12. Immerse slides in dH2O.

13. If desired, counterstain sections with hematoxylin.

14. Wash sections in dH2O two times for 5 min each.

15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each.

16. Mount sections with coverslips and mounting medium.

Datasheet & SDS

生物学的記述

Specificity
C1q Antibody [G8H3] detects endogenous levels of total C1q protein.

タンパク質の局在
細胞外環境

Uniprot ID
P02745

Clone
G8H3

Synonym(s)
Complement C1q subcomponent subunit A, C1qa

Background
C1q is a hexameric glycoprotein and the recognition molecule of the classical complement pathway, composed of 18 polypeptide chains forming six heterotrimers (C1qA, C1qB, C1qC), each with a collagen-like N-terminal domain and a globular C-terminal head (gC1q). Mainly produced by cells of the monocyte/macrophage lineage, it is also expressed by dendritic cells, endothelial cells, fibroblasts, and trophoblasts. Structurally resembling a “bouquet of tulips,” C1q plays a central role in innate and adaptive immunity by recognizing immune complexes, apoptotic cells, pathogens, and altered self-antigens via its gC1q domain, while its collagen domain interacts with receptors and serine proteases (C1r/C1s) to initiate the classical complement cascade. Beyond its complement-activating function, C1q performs diverse non-canonical roles including clearance of apoptotic cells, regulation of inflammation, neurodevelopment via synaptic pruning, pro-angiogenic activity in pregnancy and wound healing, tumor surveillance, and potential involvement in aging and neurodegeneration.

Background

C1q is a hexameric glycoprotein and the recognition molecule of the classical complement pathway, composed of 18 polypeptide chains forming six heterotrimers (C1qA, C1qB, C1qC), each with a collagen-like N-terminal domain and a globular C-terminal head (gC1q). Mainly produced by cells of the monocyte/macrophage lineage, it is also expressed by dendritic cells, endothelial cells, fibroblasts, and trophoblasts. Structurally resembling a “bouquet of tulips,” C1q plays a central role in innate and adaptive immunity by recognizing immune complexes, apoptotic cells, pathogens, and altered self-antigens via its gC1q domain, while its collagen domain interacts with receptors and serine proteases (C1r/C1s) to initiate the classical complement cascade. Beyond its complement-activating function, C1q performs diverse non-canonical roles including clearance of apoptotic cells, regulation of inflammation, neurodevelopment via synaptic pruning, pro-angiogenic activity in pregnancy and wound healing, tumor surveillance, and potential involvement in aging and neurodegeneration.

References
[1] Kouser L, et al. Front Immunol. 2015 Jun 29;6:317. [2] Reid KBM. Front Immunol. 2018 Apr 10;9:764.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%