Caspase-1 Antibody [C2H20]

Catalog No.: F4192

Application: Reactivity: Mouse

Lane 1: A20, Lane 2: EL4, Lane 3: J774A.1

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:200

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
48 kDa, 10 kDa

Datasheet & SDS

生物学的記述

Specificity
Caspase-1 Antibody [C2H20] detects endogenous levels of total Caspase-1 protein.

Clone
C2H20

Synonym(s)
Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; p45; CASP1; IL1BC; IL1BCE

Background
Caspase-1 (interleukin-1β converting enzyme, ICE), classified as an inflammatory cysteine protease of the caspase family alongside caspases-4, -5, -11, and -12, is synthesized as an inactive 45 kDa proenzyme essential for innate immune responses. It features an N-terminal caspase recruitment domain (CARD) for inflammasome assembly, a large p20 subunit containing the catalytic cysteine residue, and a small p10 subunit, which together form an active heterotetramer (p20)₂(p10)₂ through proximity-induced dimerization and autoproteolytic cleavage. Caspase-1 activates within multiprotein inflammasomes (NLRP3, AIM2, NLRC4/ASC) triggered by pathogen-associated molecular patterns or damage signals, specifically cleaving pro-IL-1β and pro-IL-18 into mature proinflammatory cytokines that drive fever, inflammation, and adaptive immunity, while processing gasdermin D to execute pyroptosis via N-terminal pore formation, causing lytic cell death and IL-1 release. Caspase-1 deficiency selectively impairs cytokine maturation and confers resistance to endotoxic shock without affecting apoptosis.

Background

Caspase-1 (interleukin-1β converting enzyme, ICE), classified as an inflammatory cysteine protease of the caspase family alongside caspases-4, -5, -11, and -12, is synthesized as an inactive 45 kDa proenzyme essential for innate immune responses. It features an N-terminal caspase recruitment domain (CARD) for inflammasome assembly, a large p20 subunit containing the catalytic cysteine residue, and a small p10 subunit, which together form an active heterotetramer (p20)₂(p10)₂ through proximity-induced dimerization and autoproteolytic cleavage. Caspase-1 activates within multiprotein inflammasomes (NLRP3, AIM2, NLRC4/ASC) triggered by pathogen-associated molecular patterns or damage signals, specifically cleaving pro-IL-1β and pro-IL-18 into mature proinflammatory cytokines that drive fever, inflammation, and adaptive immunity, while processing gasdermin D to execute pyroptosis via N-terminal pore formation, causing lytic cell death and IL-1 release. Caspase-1 deficiency selectively impairs cytokine maturation and confers resistance to endotoxic shock without affecting apoptosis.

References
[1] Molla MD, et al. J Inflamm Res. 2020 Oct 20;13:749-764. [2] Franchi L,et al. Nat Immunol. 2009 Mar;10(3):241-7.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%