Caspase-7 Antibody [H18L1]

Catalog No.: F0473

Application: Reactivity: Human, Mouse, Rat

Lane 1: A20, Lane 2: A20 (Etoposide, 25 μM, overnight)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
20 kDa, 35 kDa

Datasheet & SDS

生物学的記述

Specificity
Caspase-7 Antibody [H18L1] detects endogenous levels of total Caspase-7 protein.

Clone
H18L1

Synonym(s)
Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3; CASP7; MCH3

Background
Caspase-7 is an effector caspase belonging to the cysteine aspartate protease family, classified as a key executioner in the apoptotic cascade alongside caspase-3. It exists as an inactive proenzyme that undergoes proteolytic processing at conserved aspartic residues (Asp23, Asp198, Asp206) by upstream initiator caspases like caspase-8 or -9, yielding a large p20 subunit and small p11 subunit that heterodimerize into an active heterotetramer with a pre-formed substrate-binding pocket preferring DEVD motifs, though its apo form shows flexible loops that conform upon ligand binding. Caspase-7 cleaves essential substrates such as PARP-1 and p23 to dismantle cellular architecture during apoptosis execution in both extrinsic (death receptor-triggered) and intrinsic (mitochondrial) pathways, while also contributing to inflammation via cytokine processing and non-apoptotic roles like cell cycle regulation and membrane pore repair through acid sphingomyelinase activation. Dysregulated Caspase-7 activity influences cancer progression by modulating apoptosis resistance and promotes inflammatory disorders.

Background

Caspase-7 is an effector caspase belonging to the cysteine aspartate protease family, classified as a key executioner in the apoptotic cascade alongside caspase-3. It exists as an inactive proenzyme that undergoes proteolytic processing at conserved aspartic residues (Asp23, Asp198, Asp206) by upstream initiator caspases like caspase-8 or -9, yielding a large p20 subunit and small p11 subunit that heterodimerize into an active heterotetramer with a pre-formed substrate-binding pocket preferring DEVD motifs, though its apo form shows flexible loops that conform upon ligand binding. Caspase-7 cleaves essential substrates such as PARP-1 and p23 to dismantle cellular architecture during apoptosis execution in both extrinsic (death receptor-triggered) and intrinsic (mitochondrial) pathways, while also contributing to inflammation via cytokine processing and non-apoptotic roles like cell cycle regulation and membrane pore repair through acid sphingomyelinase activation. Dysregulated Caspase-7 activity influences cancer progression by modulating apoptosis resistance and promotes inflammatory disorders.

References
[1] Boucher D, et al. Biosci Rep. 2011 Aug;31(4):283-94. [2] Riedl SJ, et al. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):14790-5.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%