CD163L1 Antibody [C10D1]

Catalog No.: F3595

Application: Reactivity: Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:2000

Application
IHC

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

ポジティブコントロール	Tonsil macrophages tissue; Human spleen tissue; Human stomach tissue; Lung macrophages tissue; Human uterus tissue; Stomach macrophages tissue; Liver macrophages tissue
ネガティブコントロール	Placenta tissue

プロトコール

IHC
Experimental Protocol： Deparaffinization/Rehydration 1. Deparaffinize/hydrate sections: 2. Incubate sections in three washes of xylene for 5 min each. 3. Incubate sections in two washes of 100% ethanol for 10 min each. 4. Incubate sections in two washes of 95% ethanol for 10 min each. 5. Wash sections two times in dH2O for 5 min each. 6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min. Staining 1. Wash sections in dH2O three times for 5 min each. 2. Incubate sections in 3% hydrogen peroxide for 10 min. 3. Wash sections in dH2O two times for 5 min each. 4. Wash sections in wash buffer for 5 min. 5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature. 6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C. 7. Remove antibody solution and wash sections with wash buffer three times for 5 min each. 8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature. 9. Wash sections three times with wash buffer for 5 min each. 10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use. 11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity. 12. Immerse slides in dH2O. 13. If desired, counterstain sections with hematoxylin. 14. Wash sections in dH2O two times for 5 min each. 15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each. 16. Mount sections with coverslips and mounting medium.

IHC

Experimental Protocol：

Deparaffinization/Rehydration

1. Deparaffinize/hydrate sections:

2. Incubate sections in three washes of xylene for 5 min each.

3. Incubate sections in two washes of 100% ethanol for 10 min each.

4. Incubate sections in two washes of 95% ethanol for 10 min each.

5. Wash sections two times in dH2O for 5 min each.

6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.

Staining

1. Wash sections in dH2O three times for 5 min each.

2. Incubate sections in 3% hydrogen peroxide for 10 min.

3. Wash sections in dH2O two times for 5 min each.

4. Wash sections in wash buffer for 5 min.

5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature.

6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C.

7. Remove antibody solution and wash sections with wash buffer three times for 5 min each.

8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature.

9. Wash sections three times with wash buffer for 5 min each.

10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use.

11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.

12. Immerse slides in dH2O.

13. If desired, counterstain sections with hematoxylin.

14. Wash sections in dH2O two times for 5 min each.

15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each.

16. Mount sections with coverslips and mounting medium.

Datasheet & SDS

生物学的記述

Specificity
CD163L1 Antibody [C10D1] detects endogenous levels of total CD163L1 protein.

タンパク質の局在
細胞膜、細胞内膜系、細胞外環境

Uniprot ID
Q9NR16

Clone
C10D1

Synonym(s)
CD163b; CD163B; M160; UNQ6434/PRO23202; CD163L1; Scavenger receptor cysteine-rich type 1 protein M160; CD163 antigen-like 1

Background
CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

Background

CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

References
[1] Etzerodt A, et al. Antioxid Redox Signal. 2013 Jun 10;18(17):2352-63. [2] Moeller JB, et al. J Immunol. 2012 Mar 1;188(5):2399-409.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%