CHOP Antibody [C21N8]

Catalog No.: F2691

Application: Reactivity: Human, Mouse

Lane 1: HeLa, Lane 2: HeLa (Tunicamycin, 5 µg/mL, 4 h), Lane 3: HeLa (KO CHOP), Lane 4: HeLa (KO CHOP; Tunicamycin, 5 µg/mL, 4 h)

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:2000

Application
WB, IP, IHC, IF

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Observed MW
~31 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
CHOP Antibody [C21N8] detects endogenous levels of total CHOP protein.

Clone
C21N8

Synonym(s)
C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; MGC4154; CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

Background
CHOP (C/EBP Homologous Protein, also known as DDIT3 or GADD153) is a stress-inducible transcription factor of the C/EBP family that functions as a dominant-negative regulator of adipogenesis and a key mediator of ER stress-induced apoptosis. CHOP has an N-terminal transactivation/repression domain and a C-terminal bZIP domain, with proline/glycine insertions in the basic region that disrupt α-helical DNA binding and favor heterodimerization via its leucine zipper with C/EBPβ/δ or ATF/CREB family members, rather than homodimer formation. During ER stress, the PERK pathway phosphorylates eIF2α, reducing global translation but promoting ATF4 synthesis, which in turn upregulates CHOP expression. CHOP heterodimers bind C/EBP sites to repress cell cycle genes (causing G1/S arrest) and unique sites to activate pro-apoptotic targets such as GADD34 (restoring protein synthesis via eIF2α dephosphorylation), Ero1α (driving ER oxidative stress and ROS production), TRB3 (inhibiting Akt), and death receptors DR4/DR5 (triggering caspase-8 activation); CHOP can also tether AP-1 for indirect transcriptional activation. Loss of CHOP protects against ER stress-mediated apoptosis in conditions like neurodegeneration, diabetes, and ischemia, while its overexpression is implicated in cancer and fibrotic diseases.

Background

CHOP (C/EBP Homologous Protein, also known as DDIT3 or GADD153) is a stress-inducible transcription factor of the C/EBP family that functions as a dominant-negative regulator of adipogenesis and a key mediator of ER stress-induced apoptosis. CHOP has an N-terminal transactivation/repression domain and a C-terminal bZIP domain, with proline/glycine insertions in the basic region that disrupt α-helical DNA binding and favor heterodimerization via its leucine zipper with C/EBPβ/δ or ATF/CREB family members, rather than homodimer formation. During ER stress, the PERK pathway phosphorylates eIF2α, reducing global translation but promoting ATF4 synthesis, which in turn upregulates CHOP expression. CHOP heterodimers bind C/EBP sites to repress cell cycle genes (causing G1/S arrest) and unique sites to activate pro-apoptotic targets such as GADD34 (restoring protein synthesis via eIF2α dephosphorylation), Ero1α (driving ER oxidative stress and ROS production), TRB3 (inhibiting Akt), and death receptors DR4/DR5 (triggering caspase-8 activation); CHOP can also tether AP-1 for indirect transcriptional activation. Loss of CHOP protects against ER stress-mediated apoptosis in conditions like neurodegeneration, diabetes, and ischemia, while its overexpression is implicated in cancer and fibrotic diseases.

References
[1] Yang Y, et al. Front Immunol. 2017 Nov 27;8:1612. [2] Oyadomari S, et al. Cell Death Differ. 2004 Apr;11(4):381-9.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%