DEC1 Antibody [D7J6]

Catalog No.: F4322

Application: Reactivity: Human, Mouse, Rat

Lane 1: HepG2

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:100-1:1000 IP1:100-1:200

Application
WB, IP, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
43 kDa

Datasheet & SDS

生物学的記述

Specificity
DEC1 Antibody [D7J6] detects endogenous levels of total DEC1 protein.

Clone
D7J6

Synonym(s)
Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2 (bHLHb2); DEC1; Enhancer-of-split and hairy-related protein 2 (SHARP-2); BHLHE40; BHLHB2; DEC1; SHARP2; STRA13

Background
DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

Background

DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

References
[1] Sato F, et al. J Cancer. 2016 Jan 1;7(2):153-9. [2] Li Y, et al. Biochem J. 2002 Oct 15;367(Pt 2):413-22.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%