Desmoglein 1 Antibody [L24P16]

Catalog No.: F2693

Application: Reactivity: Human

Lane 1: Human skin

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:300-1:1000 IP1:100-1:200 IHC1:10-1:100

Application
WB, IP, IHC

Source
Mouse Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
Desmoglein 1 Antibody [L24P16] detects endogenous levels of total Desmoglein 1 protein.

Clone
L24P16

Synonym(s)
Cadherin family member 4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; Pemphigus foliaceus antigen; CDHF4; DG1; DSG; DSG1; EPKHE; EPKHIA; PPKS1; SPPK1

Background
Desmoglein 1 (DSG1) is a transmembrane glycoprotein cadherin of the desmoglein subfamily within desmosomes, predominantly expressed in the superficial granular and cornified layers of stratified epithelia, where it provides mechanical strength. DSG1 comprises a signal peptide, prodomain with RRQKR cleavage site yielding a 1000-amino acid mature protein, extracellular region with four cadherin domains (EC1-EC4) featuring Ca²⁺-binding motifs for homophilic adhesion, a transmembrane domain that drives lipid raft association via length and bulkiness, and a cytoplasmic tail with intracellular anchor (IA), proline-rich, and desmoglein-specific regions binding plakoglobin, plakophilin, and Erbin. DSG1 mediates calcium-dependent intercellular adhesion by trans-interacting EC1 domains clustered in lipid rafts, linking via plaque proteins to keratin filaments for tissue resilience under stress; its cytoplasmic tail suppresses EGFR/Erk signaling independent of adhesion, promoting keratinocyte differentiation, stratification, and barrier maturation. DSG1 TMD properties regulate desmosome assembly dynamics, plakoglobin recruitment, and cell surface stability. DSG1 autoantibodies cause pemphigus foliaceus acantholysis, mutations lead to striate palmoplantar keratoderma, and downregulation (e.g., by IL-13) impairs the esophageal barrier in eosinophilic esophagitis.

Background

Desmoglein 1 (DSG1) is a transmembrane glycoprotein cadherin of the desmoglein subfamily within desmosomes, predominantly expressed in the superficial granular and cornified layers of stratified epithelia, where it provides mechanical strength. DSG1 comprises a signal peptide, prodomain with RRQKR cleavage site yielding a 1000-amino acid mature protein, extracellular region with four cadherin domains (EC1-EC4) featuring Ca²⁺-binding motifs for homophilic adhesion, a transmembrane domain that drives lipid raft association via length and bulkiness, and a cytoplasmic tail with intracellular anchor (IA), proline-rich, and desmoglein-specific regions binding plakoglobin, plakophilin, and Erbin. DSG1 mediates calcium-dependent intercellular adhesion by trans-interacting EC1 domains clustered in lipid rafts, linking via plaque proteins to keratin filaments for tissue resilience under stress; its cytoplasmic tail suppresses EGFR/Erk signaling independent of adhesion, promoting keratinocyte differentiation, stratification, and barrier maturation. DSG1 TMD properties regulate desmosome assembly dynamics, plakoglobin recruitment, and cell surface stability. DSG1 autoantibodies cause pemphigus foliaceus acantholysis, mutations lead to striate palmoplantar keratoderma, and downregulation (e.g., by IL-13) impairs the esophageal barrier in eosinophilic esophagitis.

References
[1] Hammers CM, et al. J Clin Invest. 2013 Apr;123(4):1419-22. [2] Zimmer SE, et al. Mol Biol Cell. 2024 Dec 1;35(12):ar152.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%