EGR1 Antibody [N15L8]

Catalog No.: F0280

Application: Reactivity: Mouse, Rat, Human

Lane 1: JHH-1, Lane 2: REC-1, Lane 3: Mouse brain, Lane 4: Rat brain

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50 IF1:1600 CHIP1:50

Application
WB, IP, IF, ChIP

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
75 kDa

Datasheet & SDS

生物学的記述

Specificity
EGR1 Antibody [N15L8] detects endogenous levels of total EGR1 protein.

Clone
N15L8

Synonym(s)
Early growth response protein 1; EGR-1; AT225; Nerve growth factor-induced protein A (NGFI-A); Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24; EGR1; KROX24; ZNF225

Background
EGR1 (Early Growth Response 1) is a zinc finger transcription factor belonging to the EGR family of immediate-early genes, characterized by three Cys2-His2 zinc finger domains that enable specific binding to EGR response elements (EBS) in target gene promoters to regulate transcription. EGR1 features an N-terminal activation domain, a central repressive region, and C-terminal DNA-binding zinc finger motifs. Its promoter contains serum response elements (SREs) and CArG boxes, which facilitate rapid induction by stimuli such as growth factors via MAPK/ERK signaling. EGR1 acts as a key mediator linking extracellular signals to nuclear gene expression, driving processes such as cell proliferation, differentiation, apoptosis, and EMT through targets like cyclin D1, SNAIL, SLUG, PTEN, and p21, often via RAS-RAF-MEK-ERK-ELK1 cascades activated by ER stress or growth factors. EGR1 exhibits dual roles in cancer, acting as both an oncogene that promotes tumor proliferation, invasion, metastasis, and angiogenesis (e.g., via VEGFA) and as a suppressor that induces apoptosis. It is also dysregulated in cardiovascular conditions through inflammation and remodeling.

Background

EGR1 (Early Growth Response 1) is a zinc finger transcription factor belonging to the EGR family of immediate-early genes, characterized by three Cys2-His2 zinc finger domains that enable specific binding to EGR response elements (EBS) in target gene promoters to regulate transcription. EGR1 features an N-terminal activation domain, a central repressive region, and C-terminal DNA-binding zinc finger motifs. Its promoter contains serum response elements (SREs) and CArG boxes, which facilitate rapid induction by stimuli such as growth factors via MAPK/ERK signaling. EGR1 acts as a key mediator linking extracellular signals to nuclear gene expression, driving processes such as cell proliferation, differentiation, apoptosis, and EMT through targets like cyclin D1, SNAIL, SLUG, PTEN, and p21, often via RAS-RAF-MEK-ERK-ELK1 cascades activated by ER stress or growth factors. EGR1 exhibits dual roles in cancer, acting as both an oncogene that promotes tumor proliferation, invasion, metastasis, and angiogenesis (e.g., via VEGFA) and as a suppressor that induces apoptosis. It is also dysregulated in cardiovascular conditions through inflammation and remodeling.

References
[1] Shan J, et al. Biochim Biophys Acta Mol Cell Res. 2019 Mar;1866(3):371-381. [2] Wang B, et al. Front Oncol. 2021 Mar 24;11:642547.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%