GDF-15 Antibody [G11L21]

Catalog No.: F4427

Application: Reactivity: Human, Mouse, Rat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:100-1:1000 IF1:50-1:500

Application
WB, IP, IF, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
40 kDa (GDF-15 precursor), 30 kDa (mature GDF-15)

Datasheet & SDS

生物学的記述

Specificity
GDF-15 Antibody [G11L21] detects endogenous levels of total GDF-15 protein.

Clone
G11L21

Synonym(s)
Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein (NAG-1); NSAID-regulated gene 1 protein (NRG-1); Placental TGF-beta; GDF15; MIC1; PDF; PLAB; PTGFB

Background
GDF-15 (Growth/Differentiation Factor 15, also known as MIC-1) is a stress-inducible cytokine and divergent member of the TGF-β superfamily, synthesized as an inactive precursor that is proteolytically cleaved at a furin-like RXXR site to produce a mature, disulfide-linked dimer via a conserved cysteine knot motif. GDF-15 is secreted in its mature form and signals predominantly through the GFRAL receptor in the hindbrain, activating MAPK/ERK and SMAD pathways independently of canonical TGF-β receptors. GDF-15 is markedly upregulated in response to cellular stress, inflammation, mitochondrial dysfunction, and tissue injury, serving as a sensitive biomarker for perioperative risk, organ damage, and postoperative complications, often outperforming traditional markers like CRP or BNP. It modulates immune responses by restraining macrophage activity, protects tissue integrity through ERK/Akt signaling and mitochondrial stabilization, and exerts metabolic effects such as appetite suppression and weight loss relevant to bariatric surgery. Elevated GDF-15 indicates immune dysfunction in sepsis and contributes to cancer cachexia through p53-dependent transcriptional mechanisms.

Background

GDF-15 (Growth/Differentiation Factor 15, also known as MIC-1) is a stress-inducible cytokine and divergent member of the TGF-β superfamily, synthesized as an inactive precursor that is proteolytically cleaved at a furin-like RXXR site to produce a mature, disulfide-linked dimer via a conserved cysteine knot motif. GDF-15 is secreted in its mature form and signals predominantly through the GFRAL receptor in the hindbrain, activating MAPK/ERK and SMAD pathways independently of canonical TGF-β receptors. GDF-15 is markedly upregulated in response to cellular stress, inflammation, mitochondrial dysfunction, and tissue injury, serving as a sensitive biomarker for perioperative risk, organ damage, and postoperative complications, often outperforming traditional markers like CRP or BNP. It modulates immune responses by restraining macrophage activity, protects tissue integrity through ERK/Akt signaling and mitochondrial stabilization, and exerts metabolic effects such as appetite suppression and weight loss relevant to bariatric surgery. Elevated GDF-15 indicates immune dysfunction in sepsis and contributes to cancer cachexia through p53-dependent transcriptional mechanisms.

References
[1] Wischhusen J, et al. Front Immunol. 2020 May 19;11:951. [2] Emmerson PJ, et al. Frontiers in physiology 9 (2018): 1712.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%