GFI1 Antibody [M16D23]

Catalog No.: F7802

Application: Reactivity: Human, Mouse

Lane 1: THP-1, Lane 2: HL-60, Lane 3: Jurkat

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:100 IF1:800-1:3200 FCM1:800-1:1600

Application
WB, IP, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
55 kDa

Datasheet & SDS

生物学的記述

Specificity
GFI1 Antibody [M16D23] detects endogenous levels of total GFI1 protein.

Clone
M16D23

Synonym(s)
Zinc finger protein Gfi-1; Growth factor independent protein 1; GFI1

Background
GFI1 functions as a transcriptional repressor within the growth factor independence 1 family alongside GFI1B, playing essential roles in hematopoietic development and differentiation. The protein contains an N-terminal SNAG domain that recruits corepressor complexes such as CoREST, LSD1, and HDAC1/2 to enforce gene silencing through chromatin modifications. GFI1 binds target promoters via zinc-finger motifs, suppressing proliferation genes in hematopoietic stem cells while promoting lineage commitment in lymphoid and myeloid progenitors. Interaction with RUNX1/ETO fusion sustains leukemia maintenance by stabilizing oncogenic transcription networks, where GFI1 loss impairs leukemia initiation and progression through disrupted epigenetic repression. In Group 3 medulloblastoma, enhancer hijacking elevates GFI1 expression adjacent to super-enhancers, cooperating with MYC amplification to drive tumor formation via mutually exclusive activation patterns with GFI1B. This positions GFI1 at regulatory hubs integrating proliferation control, differentiation blocks, and oncogenic signaling in neural and blood lineages. Dysregulated high expression correlates with poor prognosis in acute myeloid leukemia subtypes bearing NPM1 mutations or FLT3-ITD, while variant alleles like S36N accelerate disease through altered repressor function.

Background

GFI1 functions as a transcriptional repressor within the growth factor independence 1 family alongside GFI1B, playing essential roles in hematopoietic development and differentiation. The protein contains an N-terminal SNAG domain that recruits corepressor complexes such as CoREST, LSD1, and HDAC1/2 to enforce gene silencing through chromatin modifications. GFI1 binds target promoters via zinc-finger motifs, suppressing proliferation genes in hematopoietic stem cells while promoting lineage commitment in lymphoid and myeloid progenitors. Interaction with RUNX1/ETO fusion sustains leukemia maintenance by stabilizing oncogenic transcription networks, where GFI1 loss impairs leukemia initiation and progression through disrupted epigenetic repression. In Group 3 medulloblastoma, enhancer hijacking elevates GFI1 expression adjacent to super-enhancers, cooperating with MYC amplification to drive tumor formation via mutually exclusive activation patterns with GFI1B. This positions GFI1 at regulatory hubs integrating proliferation control, differentiation blocks, and oncogenic signaling in neural and blood lineages. Dysregulated high expression correlates with poor prognosis in acute myeloid leukemia subtypes bearing NPM1 mutations or FLT3-ITD, while variant alleles like S36N accelerate disease through altered repressor function.

References
[1] Northcott PA, et al. Nature. 2014 Jul 24;511(7510):428-34. [2] Marneth AE, et al. Haematologica. 2018 Sep;103(9):e395-e399.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%