Histone H3 (mono methyl K9) Antibody [G13B9]

Catalog No.: F8174

Application: Reactivity: Mouse, Rat, Human

Lane 1: Hela, Lane 2: NIH/3T3

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:20000 IHC1:2000 IF1:2000 CHIP1:1000

Application
WB, IHC, IF, ChIP

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
15 kDa 15 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Histone H3 (mono methyl K9) Antibody [G13B9] detects endogenous levels of total Histone H3 protein only when it is mono‑methylated at K9.

Clone
G13B9

Synonym(s)
H3FA; HIST1H3A; H3C2; H3FL; HIST1H3B; H3C3; H3FC; HIST1H3C; H3C4; H3FB; HIST1H3D; H3C6; HIST1H3H; H3C11; H3FF; HIST1H3I; H3C12; H3FJ; HIST1H3J; H3C1; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/l; H3K9me; H3K9me1; H3K9

Background
Histone H3 lysine 9 monomethylation (H3K9me1) is a covalent post-translational modification occurring on the N-terminal tail of the core histone H3, a key component of the canonical nucleosome that organizes genomic DNA into chromatin. H3K9me1 is established by SET-domain-containing lysine methyltransferases, which can modify newly synthesized H3 during or immediately after translation to generate a pool of primed histones for chromatin incorporation. These monomethylated histones can serve as substrates for further methylation to H3K9me2 and H3K9me3, marks associated with heterochromatin formation, transcriptional repression, and the creation of silent chromatin domains. H3K9 methylation states modulate the recruitment of chromatin-associated factors, including reader proteins with aromatic-cage domains that discriminate between mono-, di-, and tri-methylated lysine. In certain plant species, a SAWADEE-like reader domain preferentially recognizes H3K9me1, indicating that this mark can act as a distinct docking site for effector complexes. H3K9 methylation can also antagonize local histone acetylation by restricting histone acetyltransferase access or activity, thereby reinforcing chromatin repression. Altered H3K9 methylation patterns, such as loss or redistribution of H3K9me1, have been linked to compromised heterochromatin maintenance, impaired transposon silencing, and gene regulation defects, contributing to developmental disorders and cancer. Positioned at the crossroads of histone turnover, hierarchical methylation, and reader recruitment, H3K9me1 serves as a key marker and functional probe for studying early heterochromatin assembly, RNA-directed DNA methylation, and the epigenetic regulation of genome stability.

Background

Histone H3 lysine 9 monomethylation (H3K9me1) is a covalent post-translational modification occurring on the N-terminal tail of the core histone H3, a key component of the canonical nucleosome that organizes genomic DNA into chromatin. H3K9me1 is established by SET-domain-containing lysine methyltransferases, which can modify newly synthesized H3 during or immediately after translation to generate a pool of primed histones for chromatin incorporation. These monomethylated histones can serve as substrates for further methylation to H3K9me2 and H3K9me3, marks associated with heterochromatin formation, transcriptional repression, and the creation of silent chromatin domains. H3K9 methylation states modulate the recruitment of chromatin-associated factors, including reader proteins with aromatic-cage domains that discriminate between mono-, di-, and tri-methylated lysine. In certain plant species, a SAWADEE-like reader domain preferentially recognizes H3K9me1, indicating that this mark can act as a distinct docking site for effector complexes. H3K9 methylation can also antagonize local histone acetylation by restricting histone acetyltransferase access or activity, thereby reinforcing chromatin repression. Altered H3K9 methylation patterns, such as loss or redistribution of H3K9me1, have been linked to compromised heterochromatin maintenance, impaired transposon silencing, and gene regulation defects, contributing to developmental disorders and cancer. Positioned at the crossroads of histone turnover, hierarchical methylation, and reader recruitment, H3K9me1 serves as a key marker and functional probe for studying early heterochromatin assembly, RNA-directed DNA methylation, and the epigenetic regulation of genome stability.

References
[1] Krishnan S, et al. Chembiochem. 2011 Jan 24;12(2):254-63. [2] Bannister AJ, et al. Cell Res. 2011 Mar;21(3):381-95.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%