MERIT40 Antibody [B22L22]

Catalog No.: F8556

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: LNCAP, Lane 3: A431, Lane 4: COS-7

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:200

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
40 kDa

Datasheet & SDS

生物学的記述

Specificity
MERIT40 Antibody [B22L22] detects endogenous levels of total MERIT40 protein.

Clone
B22L22

Synonym(s)
Mediator of RNA polymerase II transcription subunit 40; BRISC and BRCA1-A complex subunit 1; BABAM1; MERIT40

Background
MERIT40 (NBA1, Mediator of RAP80 Interactions and Targeting 40 kDa) is a scaffold protein that participates in the BRCA1‑A complex, a multi‑subunit assembly that links DNA‑damage signaling to BRCA1‑dependent repair and checkpoint control. MERIT40 contains a central coiled‑coil domain and a C‑terminal PXXR motif that mediates direct interactions with BRE/BRCC45 and other core components of the BRCA1‑A complex, including RAP80, Abraxas, BRCC36, and CBP/p300‑binding protein, and these interactions are required to maintain the stability and stoichiometry of the complex at sites of DNA double‑strand breaks. MERIT40 localizes to DNA damage‑induced nuclear foci, and its presence is essential for the proper recruitment and retention of BRCA1 at chromatin surrounding breaks, for the formation of RAP80 and BRCA1 irradiation‑induced foci, and for BRCA1‑dependent G2/M checkpoint activation and resistance to ionizing radiation. In the context of DNA‑damage signaling, MERIT40 is phosphorylated at Ser29 by Akt in response to genotoxic stress, and this phospho‑switch promotes enhanced association of MERIT40 with Rap80 and other BRCA1‑A subunits, thereby facilitating DNA‑damage‑dependent assembly of the complex and supporting efficient resolution of DNA lesions and cell‑survival outcomes. Alterations in the MERIT40‑containing BRCA1‑A complex are associated with impaired DNA‑repair capacity and genome instability.

Background

MERIT40 (NBA1, Mediator of RAP80 Interactions and Targeting 40 kDa) is a scaffold protein that participates in the BRCA1‑A complex, a multi‑subunit assembly that links DNA‑damage signaling to BRCA1‑dependent repair and checkpoint control. MERIT40 contains a central coiled‑coil domain and a C‑terminal PXXR motif that mediates direct interactions with BRE/BRCC45 and other core components of the BRCA1‑A complex, including RAP80, Abraxas, BRCC36, and CBP/p300‑binding protein, and these interactions are required to maintain the stability and stoichiometry of the complex at sites of DNA double‑strand breaks. MERIT40 localizes to DNA damage‑induced nuclear foci, and its presence is essential for the proper recruitment and retention of BRCA1 at chromatin surrounding breaks, for the formation of RAP80 and BRCA1 irradiation‑induced foci, and for BRCA1‑dependent G2/M checkpoint activation and resistance to ionizing radiation. In the context of DNA‑damage signaling, MERIT40 is phosphorylated at Ser29 by Akt in response to genotoxic stress, and this phospho‑switch promotes enhanced association of MERIT40 with Rap80 and other BRCA1‑A subunits, thereby facilitating DNA‑damage‑dependent assembly of the complex and supporting efficient resolution of DNA lesions and cell‑survival outcomes. Alterations in the MERIT40‑containing BRCA1‑A complex are associated with impaired DNA‑repair capacity and genome instability.

References
[1] Feng L, et al. Genes Dev. 2009 Mar 15;23(6):719-28. [2] Brown KK, et al. Cell Rep. 2015 Jun 9;11(9):1358-66.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%