MTA1 Antibody [F4J14]

Catalog No.: F4782

Application: Reactivity: Human

Lane 1: Hela, Lane 2: 293T, Lane 3: MCF7, Lane 4: A549

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IF1:50 FCM1:500

Application
WB, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
81 kDa 78-82 kDa, 85 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
MTA1 Antibody [F4J14] detects endogenous levels of total MTA1 protein.

Clone
F4J14

Synonym(s)
Metastasis-associated protein MTA1; MTA1

Background
MTA1 (Metastasis-Associated Protein 1) is an essential scaffold component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, a chromatin-modifying corepressor belonging to the metastasis-associated (MTA) family of transcriptional regulators. MTA1 features conserved domains, including an N-terminal BAH domain forming a β-barrel core with insertions for histone H4 recognition, a central zinc-finger motif (C2HC), the ELM2 domain with a basic pocket for HDAC1 activation via helix 3 of the adjacent SANT domain, and a C-terminal KRAARR motif modulating RBBP4 binding; intrinsically disordered regions become ordered upon interaction to stabilize NuRD assembly. MTA1 facilitates NuRD-mediated histone deacetylation and nucleosome remodeling by directly contacting HDACs, RBBP4/7, and Mi-2, enabling recruitment to gene loci for epigenetic silencing. Functionally, it activates class I HDACs through inositol phosphate binding at the ELM2-SANT interface, regulates mitosis by binding transcripts at splicing motifs to control alternative splicing of regulators like ATRX and MYBL2, and influences SAC components (e.g., MAD1/2) for proper chromosome segregation. MTA1 deletion causes defective mitotic arrest, chromosomal instability, and tumorigenesis, with overexpression linked to cancer progression via hypoxia-induced HIF-1α stabilization and Wnt/β-catenin modulation.

Background

MTA1 (Metastasis-Associated Protein 1) is an essential scaffold component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, a chromatin-modifying corepressor belonging to the metastasis-associated (MTA) family of transcriptional regulators. MTA1 features conserved domains, including an N-terminal BAH domain forming a β-barrel core with insertions for histone H4 recognition, a central zinc-finger motif (C2HC), the ELM2 domain with a basic pocket for HDAC1 activation via helix 3 of the adjacent SANT domain, and a C-terminal KRAARR motif modulating RBBP4 binding; intrinsically disordered regions become ordered upon interaction to stabilize NuRD assembly. MTA1 facilitates NuRD-mediated histone deacetylation and nucleosome remodeling by directly contacting HDACs, RBBP4/7, and Mi-2, enabling recruitment to gene loci for epigenetic silencing. Functionally, it activates class I HDACs through inositol phosphate binding at the ELM2-SANT interface, regulates mitosis by binding transcripts at splicing motifs to control alternative splicing of regulators like ATRX and MYBL2, and influences SAC components (e.g., MAD1/2) for proper chromosome segregation. MTA1 deletion causes defective mitotic arrest, chromosomal instability, and tumorigenesis, with overexpression linked to cancer progression via hypoxia-induced HIF-1α stabilization and Wnt/β-catenin modulation.

References
[1] Millard CJ, et al. Cancer Metastasis Rev. 2014 Dec;33(4):857-67. [2] Millard CJ, et al. Elife. 2016 Apr 21;5:e13941.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%