MTA2 Antibody [P2H17]

Catalog No.: F5114

Application: Reactivity: Human, Mouse, Rat

Lane 1: SW480, Lane 2: Jurkat, Lane 3: K562, Lane 4: NIH/3T3

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:5000-1:50000 IHC1:500-1:2000 IF1:1000-1:2000 FCM1:10000

Application
WB, IHC, IF, FCM, ELISA

Source
Mouse Monoclonal Antibody

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
75 kDa 75 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
MTA2 Antibody [P2H17] detects endogenous levels of total MTA2 protein.

Clone
P2H17

Synonym(s)
Metastasis-associated protein MTA2; Metastasis-associated 1-like 1 (MTA1-L1 protein); p53 target protein in deacetylase complex; MTA2; MTA1L1; PID

Background
MTA2 forms part of the MTA family within the NuRD chromatin remodeling and histone deacetylase complex, where it scaffolds interactions essential for transcriptional repression across diverse cellular programs. MTA2 features a zinc finger domain alongside BAH and SANT motifs that facilitate DNA and protein binding, enabling its integration into distinct NuRD variants containing MBD3, Sin3a, and CHD4 for targeted chromatin modification. Through these associations, MTA2 recruits HDAC enzymatic cores to deacetylate histones at promoter regions, compacting chromatin and silencing genes involved in differentiation and hormone response pathways. MTA2 directly engages ERα to suppress estrogen-responsive transcription, altering receptor conformation and cofactor recruitment to shift cells toward resistant phenotypes in hormone-dependent contexts. MTA2 collaborates with transcription factors like Aiolos and Ikaros in B cell progenitors, modulating lineage-specific gene networks by enforcing repressive epigenetic marks on developmental loci. This activity extends to broader lineage commitment, where MTA2-containing NuRD complexes balance progenitor proliferation against maturation cues through cyclic modulation of acetyl marks on key regulatory elements.

Background

MTA2 forms part of the MTA family within the NuRD chromatin remodeling and histone deacetylase complex, where it scaffolds interactions essential for transcriptional repression across diverse cellular programs. MTA2 features a zinc finger domain alongside BAH and SANT motifs that facilitate DNA and protein binding, enabling its integration into distinct NuRD variants containing MBD3, Sin3a, and CHD4 for targeted chromatin modification. Through these associations, MTA2 recruits HDAC enzymatic cores to deacetylate histones at promoter regions, compacting chromatin and silencing genes involved in differentiation and hormone response pathways. MTA2 directly engages ERα to suppress estrogen-responsive transcription, altering receptor conformation and cofactor recruitment to shift cells toward resistant phenotypes in hormone-dependent contexts. MTA2 collaborates with transcription factors like Aiolos and Ikaros in B cell progenitors, modulating lineage-specific gene networks by enforcing repressive epigenetic marks on developmental loci. This activity extends to broader lineage commitment, where MTA2-containing NuRD complexes balance progenitor proliferation against maturation cues through cyclic modulation of acetyl marks on key regulatory elements.

References
[1] Thivakaran A, et al. Haematologica. 2018 Apr;103(4):614-625. [2] Liu L, et al. Leukemia. 2022 Sep;36(9):2196-2207.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%