Mucin 5AC Antibody [L15D9]

Catalog No.: F1564

Application: Reactivity: Mouse, Rat, Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
IHC1:100 - 1:5000 IF1:100

Application
IHC, IF

Source
Mouse Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

生物学的記述

Specificity
Mucin 5AC Antibody [L15D9] detects endogenous levels of total Mucin 5AC protein.

Clone
L15D9

Synonym(s)
MUC5; MUC5AC; Mucin-5AC; MUC-5AC; Gastric mucin; Major airway glycoprotein; Tracheobronchial mucin; TBM

Background
Mucin 5AC (MUC5AC) is a secreted, gel-forming mucin glycoprotein belonging to the mucin family, predominantly expressed by goblet cells in the respiratory, gastric, and conjunctival epithelia, where it constitutes the primary component of protective mucus gels. MUC5AC is a massive multidomain protein featuring N- and C-terminal globular regions with von Willebrand factor (VWF)-like D-domains (D1, D2, D2', D3, D4), cysteine-rich subdomains (CysD1-9), and a central proline/threonine/serine-rich (PTS) mucin domain densely O-glycosylated (>80% mass as carbohydrate) that extends into rigid rod-like structures; N-terminal D1-D3 domains mediate multimerization into tightly branched networks via disulfide bonds and homotypic interactions. MUC5AC polymerizes with MUC5B to form viscoelastic mucus layers that trap pathogens, facilitate mucociliary clearance, lubricate epithelia, and bind water for barrier hydration; its biophysical properties create stiffer, denser gels compared to MUC5B due to extensive branching. MUC5AC expression is upregulated via EGFR and IL-13/STAT6 pathways during inflammation, contributing to airway obstruction in asthma, COPD, and cystic fibrosis, where hypersecretion impairs clearance; it also promotes tumor progression in lung adenocarcinoma via ANXA2 signaling.

Background

Mucin 5AC (MUC5AC) is a secreted, gel-forming mucin glycoprotein belonging to the mucin family, predominantly expressed by goblet cells in the respiratory, gastric, and conjunctival epithelia, where it constitutes the primary component of protective mucus gels. MUC5AC is a massive multidomain protein featuring N- and C-terminal globular regions with von Willebrand factor (VWF)-like D-domains (D1, D2, D2', D3, D4), cysteine-rich subdomains (CysD1-9), and a central proline/threonine/serine-rich (PTS) mucin domain densely O-glycosylated (>80% mass as carbohydrate) that extends into rigid rod-like structures; N-terminal D1-D3 domains mediate multimerization into tightly branched networks via disulfide bonds and homotypic interactions. MUC5AC polymerizes with MUC5B to form viscoelastic mucus layers that trap pathogens, facilitate mucociliary clearance, lubricate epithelia, and bind water for barrier hydration; its biophysical properties create stiffer, denser gels compared to MUC5B due to extensive branching. MUC5AC expression is upregulated via EGFR and IL-13/STAT6 pathways during inflammation, contributing to airway obstruction in asthma, COPD, and cystic fibrosis, where hypersecretion impairs clearance; it also promotes tumor progression in lung adenocarcinoma via ANXA2 signaling.

References
[1] Krishn SR, et al. Carcinogenesis. 2018 May 3;39(5):633-651. [2] Carpenter J, et al. Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2104490118.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%