Myosin Light Chain 2 Antibody [C23E15]

Catalog No.: F3588

Application: Reactivity: Mouse, Rat, Human

Lane 1: Human heart, Lane 2: Human skeletal muscle, Lane 3: Mouse heart, Lane 4: Rat heart

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:10000 IHC1:600

Application
WB, IHC

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
19 kDa 19 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Myosin Light Chain 2 Antibody [C23E15] detects endogenous levels of total Myosin Light Chain 2 protein.

Clone
C23E15

Synonym(s)
MLC2; MYL2; MLC-2; MLC-2v; Cardiac myosin light chain 2; Ventricular myosin light chain 2; MLC-2s/v

Background
Myosin Light Chain 2 (MLC2, MYL2) is a 166-amino acid EF-hand Ca²⁺-binding regulatory light chain specifically expressed in cardiac (MLC2v) and smooth muscle, where it adopts a compact globular fold with two N-terminal EF-hand motifs for Ca²⁺/Mg²⁺ binding and a C-terminal domain that interacts with the IQ1 motif in myosin heavy chain’s neck region, working alongside the essential light chain (MLC1) at the motor-neck junction. The key phosphorylation site Ser19 is located in a flexible loop. MLC2 stabilizes myosin thick filaments and modulates actomyosin cross-bridge kinetics; phosphorylation of Ser19 by Ca²⁺/calmodulin-activated MLCK (and also by ROCK) induces a conformational switch to an extended myosin form, increasing lever arm stiffness, slowing detachment, prolonging the duty cycle, and enhancing Ca²⁺ sensitivity, thus boosting force generation during cardiac and smooth muscle contraction. Dephosphorylation by myosin phosphatase (MYPT1-PP1) enables relaxation, while non-muscle MLC2 plays roles in cytokinesis and stress fiber regulation. Mutations or knockout of MLC2 cause hypertrophic or dilated cardiomyopathy with thick filament disorganization and heart failure, and altered phosphorylation is linked to hypertension.

Background

Myosin Light Chain 2 (MLC2, MYL2) is a 166-amino acid EF-hand Ca²⁺-binding regulatory light chain specifically expressed in cardiac (MLC2v) and smooth muscle, where it adopts a compact globular fold with two N-terminal EF-hand motifs for Ca²⁺/Mg²⁺ binding and a C-terminal domain that interacts with the IQ1 motif in myosin heavy chain’s neck region, working alongside the essential light chain (MLC1) at the motor-neck junction. The key phosphorylation site Ser19 is located in a flexible loop. MLC2 stabilizes myosin thick filaments and modulates actomyosin cross-bridge kinetics; phosphorylation of Ser19 by Ca²⁺/calmodulin-activated MLCK (and also by ROCK) induces a conformational switch to an extended myosin form, increasing lever arm stiffness, slowing detachment, prolonging the duty cycle, and enhancing Ca²⁺ sensitivity, thus boosting force generation during cardiac and smooth muscle contraction. Dephosphorylation by myosin phosphatase (MYPT1-PP1) enables relaxation, while non-muscle MLC2 plays roles in cytokinesis and stress fiber regulation. Mutations or knockout of MLC2 cause hypertrophic or dilated cardiomyopathy with thick filament disorganization and heart failure, and altered phosphorylation is linked to hypertension.

References
[1] Sheikh F, et al. Gene. 2015 Sep 10;569(1):14-20. [2] Wang L, et al. J Gen Physiol. 2021 Mar 1;153(3):e202012781.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%