Phospho-Histone H2A (Ser129) Antibody [A4N9]

Catalog No.: F3871

Application: Reactivity: Human, Saccharomyces cerevisiae

Lane 1: Saccharomyces cerevisiae (0.2% Methyl methanesulfonate, 1 h), Lane 2: Saccharomyces cerevisiae

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:5000 CHIP1:500

Application
WB, ChIP, ELISA

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Saccharomyces cerevisiae

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
14 kDa 14 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-Histone H2A (Ser129) Antibody [A4N9] detects endogenous levels of total Histone H2A protein only when it is phosphorylated at Ser129.

Clone
A4N9

Synonym(s)
H2A2; YBL003C; YBL0103; HTA2; Histone H2A.2

Background
Phospho Histone H2A at Ser129 (H2A S129ph) is a conserved, DNA damage–induced histone mark that plays a central role in the chromatin based signaling cascade coordinating double strand break (DSB) repair and genome integrity, particularly in yeast and mammalian systems where it functions as part of the broader H2A.X family of variant histones. The modification occurs at a C terminal SQ motif that is phosphorylated by phosphoinositide 3 kinase related kinases such as ATM and ATR in response to DNA breaks, generating a localized phospho epitope that spreads over kilobase scale chromatin regions flanking the lesion and thereby serving as a nucleation platform for downstream repair factors. H2A S129ph directly recruits chromatin modifying complexes such as the NuA4 histone acetyltransferase and Ino80/Swr1 type ATP dependent remodeling complexes, which are anchored through specific subunits that recognize the phosphorylated tail, thus promoting acetylation and restructuring of nearby nucleosomes to enhance accessibility of damaged DNA to repair enzymes. This phospho H2A–dependent recruitment supports efficient homologous recombination and non homologous end joining by facilitating the assembly and retention of DSB sensing and processing factors at the break site, while also interfacing with cell cycle checkpoints to delay progression until repair is complete. H2A S129ph is widely used as a sensitive, high resolution readout for DSB generation and repair efficiency, and dysregulation of its phosphorylation or recognition machinery contributes to defective repair responses, increased genomic instability, and hypersensitivity to genotoxic agents, so that H2A S129ph dependent signaling is ultimately dysregulated in DNA repair deficient and cancer prone backgrounds.

Background

Phospho Histone H2A at Ser129 (H2A S129ph) is a conserved, DNA damage–induced histone mark that plays a central role in the chromatin based signaling cascade coordinating double strand break (DSB) repair and genome integrity, particularly in yeast and mammalian systems where it functions as part of the broader H2A.X family of variant histones. The modification occurs at a C terminal SQ motif that is phosphorylated by phosphoinositide 3 kinase related kinases such as ATM and ATR in response to DNA breaks, generating a localized phospho epitope that spreads over kilobase scale chromatin regions flanking the lesion and thereby serving as a nucleation platform for downstream repair factors. H2A S129ph directly recruits chromatin modifying complexes such as the NuA4 histone acetyltransferase and Ino80/Swr1 type ATP dependent remodeling complexes, which are anchored through specific subunits that recognize the phosphorylated tail, thus promoting acetylation and restructuring of nearby nucleosomes to enhance accessibility of damaged DNA to repair enzymes. This phospho H2A–dependent recruitment supports efficient homologous recombination and non homologous end joining by facilitating the assembly and retention of DSB sensing and processing factors at the break site, while also interfacing with cell cycle checkpoints to delay progression until repair is complete. H2A S129ph is widely used as a sensitive, high resolution readout for DSB generation and repair efficiency, and dysregulation of its phosphorylation or recognition machinery contributes to defective repair responses, increased genomic instability, and hypersensitivity to genotoxic agents, so that H2A S129ph dependent signaling is ultimately dysregulated in DNA repair deficient and cancer prone backgrounds.

References
[1] Downs JA, et al. Mol Cell. 2004 Dec 22;16(6):979-90. [2] Fink M, et al. Mol Cell Biol. 2007 May;27(10):3589-600.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%