Phospho-MOB1 (Thr12) Antibody [F15P10]

Catalog No.: F9786

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Ramos, Lane 2: Ramos (λ phosphatase treated)

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Mouse, Rat, Monkey

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
24 kDa

Datasheet & SDS

生物学的記述

Specificity
Phospho-MOB1 (Thr12) Antibody [F15P10] detects endogenous levels of total MOB1 protein only when it is phosphorylated at Thr12.

Clone
F15P10

Synonym(s)
MOB kinase activator 1A; Mob1α; MOBKL1A; MOB1A

Background
Phospho-MOB1 (Thr12) designates the activated conformation of MOB1A/B adaptor proteins central to the Hippo tumor suppressor pathway that governs organ size, proliferation, and apoptosis through kinase cascade amplification. MOB1 organizes a compact globular fold with an N-terminal extension and core domain that undergoes phosphorylation at Thr12 by MST1/2 kinases, stabilizing an open conformation where the Switch helix dissociates to expose binding interfaces for LATS1/2 N-terminal regulatory regions. This phospho-Thr12 event allosterically activates LATS1/2 catalytic domains through dynamic scaffolding that juxtaposes MST-phosphorylated activation loops with MOB1 phospho-sites, enabling trans-phosphorylation that ramps up YAP/TAZ inhibitory serine phosphorylation for cytoplasmic retention and proteasomal degradation via β-TRCP ubiquitin ligase. Phospho-MOB1 further engages NDR1/2 kinases with differential affinity modulated by Thr12/Thr35 phosphates that pull N-terminal strands into β-sheet configurations stabilizing kinase-MOB complexes for mitotic exit and ploidy control. The resultant LATS-YAP/TAZ axis integrates upstream inputs, such as cell density and mechanotransduction, through Merlin-expanded sensing to transcriptionally repress CTGF, Cyr61, and c-Myc, thereby facilitating contact inhibition during epithelial morphogenesis. Phospho-MOB1 coordinates tissue homeostasis in liver regeneration and neural tube closure, with ubiquitous expression ensuring robust pathway fidelity across proliferative niches.

Background

Phospho-MOB1 (Thr12) designates the activated conformation of MOB1A/B adaptor proteins central to the Hippo tumor suppressor pathway that governs organ size, proliferation, and apoptosis through kinase cascade amplification. MOB1 organizes a compact globular fold with an N-terminal extension and core domain that undergoes phosphorylation at Thr12 by MST1/2 kinases, stabilizing an open conformation where the Switch helix dissociates to expose binding interfaces for LATS1/2 N-terminal regulatory regions. This phospho-Thr12 event allosterically activates LATS1/2 catalytic domains through dynamic scaffolding that juxtaposes MST-phosphorylated activation loops with MOB1 phospho-sites, enabling trans-phosphorylation that ramps up YAP/TAZ inhibitory serine phosphorylation for cytoplasmic retention and proteasomal degradation via β-TRCP ubiquitin ligase. Phospho-MOB1 further engages NDR1/2 kinases with differential affinity modulated by Thr12/Thr35 phosphates that pull N-terminal strands into β-sheet configurations stabilizing kinase-MOB complexes for mitotic exit and ploidy control. The resultant LATS-YAP/TAZ axis integrates upstream inputs, such as cell density and mechanotransduction, through Merlin-expanded sensing to transcriptionally repress CTGF, Cyr61, and c-Myc, thereby facilitating contact inhibition during epithelial morphogenesis. Phospho-MOB1 coordinates tissue homeostasis in liver regeneration and neural tube closure, with ubiquitous expression ensuring robust pathway fidelity across proliferative niches.

References
[1] Kim SY, et al. Sci Rep. 2016 Jun 23;6:28488. [2] Ni L, et al. Genes Dev. 2015 Jul 1;29(13):1416-31.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%