Phospho-SHP2 (Tyr542) Antibody [J17K6]

Catalog No.: F2201

Application: Reactivity: Mouse, Human

Lane 1: NIH/3T3, Lane 2: NIH/3T3 (PDGF, 40ng/ml, 40 min), Lane 3: NIH/3T3 (PDGF, 40ng/ml, 40 min; phosphatase-treated)

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:30 IF1:100 - 1:250

Application
WB, IP, IF

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
68 kDa 68 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-SHP2 (Tyr542) Antibody [J17K6] detects endogenous levels of total SHP2 protein only when it is Phosphorylated at Tyr542.

Clone
J17K6

Synonym(s)
PTP2C; SHPTP2; PTPN11; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3; PTP-1D; PTP-2C; SHP-2; Shp2

Background
Phospho-SHP2 (Tyr542) refers to the tyrosine-phosphorylated form at residue Tyr542 of SHP2, also known as PTPN11, a non-receptor protein tyrosine phosphatase (PTP) belonging to the classical PTP family. SHP2 comprises two N-terminal SH2 domains (N-SH2 and C-SH2), a catalytic PTP domain, and C-terminal tyrosine residues including Tyr542 and Tyr580; phosphorylation at Tyr542 enables intramolecular interaction with the N-SH2 domain, relieving autoinhibition and exposing the active site. Phosphorylated Tyr542 also binds Grb2, acting as a scaffold to recruit signaling complexes independent of phosphatase activity. Phospho-SHP2 (Tyr542) activates upon growth factor stimulation via kinases like EphA2, promoting sustained ERK activation in Ras/MAPK pathways and modulating PI3K/AKT signaling through Gab1 dephosphorylation. It coordinates cellular responses to growth factors, cytokines, and hormones, regulating proliferation, differentiation, migration, and survival. Dysregulated Phospho-SHP2 (Tyr542) contributes to cancers via enhanced ERK signaling and to LEOPARD syndrome through aberrant MAPK activation.

Background

Phospho-SHP2 (Tyr542) refers to the tyrosine-phosphorylated form at residue Tyr542 of SHP2, also known as PTPN11, a non-receptor protein tyrosine phosphatase (PTP) belonging to the classical PTP family. SHP2 comprises two N-terminal SH2 domains (N-SH2 and C-SH2), a catalytic PTP domain, and C-terminal tyrosine residues including Tyr542 and Tyr580; phosphorylation at Tyr542 enables intramolecular interaction with the N-SH2 domain, relieving autoinhibition and exposing the active site. Phosphorylated Tyr542 also binds Grb2, acting as a scaffold to recruit signaling complexes independent of phosphatase activity. Phospho-SHP2 (Tyr542) activates upon growth factor stimulation via kinases like EphA2, promoting sustained ERK activation in Ras/MAPK pathways and modulating PI3K/AKT signaling through Gab1 dephosphorylation. It coordinates cellular responses to growth factors, cytokines, and hormones, regulating proliferation, differentiation, migration, and survival. Dysregulated Phospho-SHP2 (Tyr542) contributes to cancers via enhanced ERK signaling and to LEOPARD syndrome through aberrant MAPK activation.

References
[1] Anselmi M, et al. J Chem Inf Model. 2020 Jun 22;60(6):3157-3171. [2] Miura K, et al. Oncogene. 2013 Nov 7;32(45):5292-301.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%