Phospho-Src (Tyr419) Antibody [D21H6]

Catalog No.: F2480

Application: Reactivity: Mouse, Rat, Human

Lane 1: A431, Lane 2: A431 (Pervanadate, 50 mM, 5 min), Lane 3: NIH/3T3, Lane 4: NIH/3T3 (Pervanadate, 50 mM, 5 min)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:5000 FCM1:100

Application
WB, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
60 kDa 60 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-Src (Tyr419) Antibody [D21H6] detects endogenous levels of total Src protein only when it is phosphorylated at Tyr419.

Clone
D21H6

Synonym(s)
SRC1; SRC; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Background
Phospho-Src (Tyr419), the activation loop phosphorylation marking full catalytic competence of the Src family kinase prototype, unlocks bidirectional tyrosine kinase signaling from virtually all integrin, growth factor, and GPCR stimuli across adherent cells and platelets. Dephosphorylation of inhibitory Tyr530 by CD45/PTP1B relieves SH2 domain clamping, permitting trans-autophosphorylation at Tyr419 that rigidly positions the activation loop for substrate access while relieving steric occlusion of the ATP-binding cleft; this catalyzes hierarchical phosphorylation cascades where Src primes receptor tyrosine kinases through Y1009/1101 autophosphorylation on EGFR while amplifying FAK at Y397/576/577/861 to scaffold paxillin/PI3K recruitment. Active p-Tyr419-Src drives Ras-GRF1/ERK for proliferation, STAT3/5a for survival, and cortactin/pyk2 for invadopodia maturation via sequential Arp2/3 activation; feedback engages Csk/Shp1 through C-terminal docking to restore Tyr530 phosphorylation, while PP2A/PP1 dephosphorylate the activation loop for signal termination. In platelets, shear-induced clustering at podosome-like adhesions generates local p-Tyr419 gradients that license αIIbβ3 outside-in signaling for clot retraction, while fibroblasts exploit Src for durotaxis through myosin-IIA/Y1183 feedback with tension sensors.

Background

Phospho-Src (Tyr419), the activation loop phosphorylation marking full catalytic competence of the Src family kinase prototype, unlocks bidirectional tyrosine kinase signaling from virtually all integrin, growth factor, and GPCR stimuli across adherent cells and platelets. Dephosphorylation of inhibitory Tyr530 by CD45/PTP1B relieves SH2 domain clamping, permitting trans-autophosphorylation at Tyr419 that rigidly positions the activation loop for substrate access while relieving steric occlusion of the ATP-binding cleft; this catalyzes hierarchical phosphorylation cascades where Src primes receptor tyrosine kinases through Y1009/1101 autophosphorylation on EGFR while amplifying FAK at Y397/576/577/861 to scaffold paxillin/PI3K recruitment. Active p-Tyr419-Src drives Ras-GRF1/ERK for proliferation, STAT3/5a for survival, and cortactin/pyk2 for invadopodia maturation via sequential Arp2/3 activation; feedback engages Csk/Shp1 through C-terminal docking to restore Tyr530 phosphorylation, while PP2A/PP1 dephosphorylate the activation loop for signal termination. In platelets, shear-induced clustering at podosome-like adhesions generates local p-Tyr419 gradients that license αIIbβ3 outside-in signaling for clot retraction, while fibroblasts exploit Src for durotaxis through myosin-IIA/Y1183 feedback with tension sensors.

References
[1] Irby RB, et al. Nat Genet. 1999 Feb;21(2):187-90. [2] Irtegun S, et al. PLoS One. 2013 Jul 26;8(7):e71035.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%