Phospho-Syk (Tyr323) Antibody [G1N3]

Catalog No.: F3319

Application: Reactivity: Human

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 - 1:2000 IHC1:100 - 1:250 IF1:100 - 1:250 FCM1:2500

Application
WB, IHC, IF, FCM

Source
Rabbit Monoclonal Antibody

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
72 kDa 72 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
Phospho-Syk (Tyr323) Antibody [G1N3] detects endogenous levels of total Syk protein only when it is phosphorylated at Tyr323.

Clone
G1N3

Synonym(s)
Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK

Background
Phospho-Syk (Tyr323) refers to Spleen Tyrosine Kinase (Syk), a 72 kDa non-receptor tyrosine kinase, phosphorylated at Tyr323 within interdomain B (the SH2-kinase linker), a modification critical for immune signaling regulation. Syk consists of a tandem N-terminal SH2 domain, interdomain A, interdomain B (containing Tyr323/326, which form a Cbl-binding phosphotyrosine motif), and a C-terminal kinase domain with an activation loop (Tyr525/526). Upon activation of immune receptors such as BCR, FcεR, or ITAMs, Src kinases phosphorylate ITAMs, recruiting Syk via its SH2 domains, after which Syk autophosphorylates at Tyr323, this both relieves autoinhibition and recruits Cbl ubiquitin ligase for feedback inhibition. Further phosphorylation events activate Syk’s kinase activity, triggering downstream signaling through PLCγ2, LAT, BLNK, PI3K, and MAPK/ERK pathways, ultimately driving calcium flux, NF-κB/AP-1 activation, degranulation, phagocytosis, and B-cell maturation. Syk plays a central role in orchestrating both innate and adaptive immunity; persistent Tyr323 phosphorylation is associated with sustained signaling in autoimmune diseases like rheumatoid arthritis and B-cell lymphomas.

Background

Phospho-Syk (Tyr323) refers to Spleen Tyrosine Kinase (Syk), a 72 kDa non-receptor tyrosine kinase, phosphorylated at Tyr323 within interdomain B (the SH2-kinase linker), a modification critical for immune signaling regulation. Syk consists of a tandem N-terminal SH2 domain, interdomain A, interdomain B (containing Tyr323/326, which form a Cbl-binding phosphotyrosine motif), and a C-terminal kinase domain with an activation loop (Tyr525/526). Upon activation of immune receptors such as BCR, FcεR, or ITAMs, Src kinases phosphorylate ITAMs, recruiting Syk via its SH2 domains, after which Syk autophosphorylates at Tyr323, this both relieves autoinhibition and recruits Cbl ubiquitin ligase for feedback inhibition. Further phosphorylation events activate Syk’s kinase activity, triggering downstream signaling through PLCγ2, LAT, BLNK, PI3K, and MAPK/ERK pathways, ultimately driving calcium flux, NF-κB/AP-1 activation, degranulation, phagocytosis, and B-cell maturation. Syk plays a central role in orchestrating both innate and adaptive immunity; persistent Tyr323 phosphorylation is associated with sustained signaling in autoimmune diseases like rheumatoid arthritis and B-cell lymphomas.

References
[1] Cottat M, et al. Sci Rep. 2017 Jan 5;7:39766. [2] Buitrago L, et al. J Biol Chem. 2013 Oct 4;288(40):29160-9.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%