Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) Antibody [K13K11]

Catalog No.: F0403

Application: Reactivity: Human, Rat

Lane 1: NIH/3T3 (transfected with TrkA), Lane 2: NIH/3T3 (transfected with TrkA; NGF-treated), Lane 3: NIH/3T3 (transfected with TrkB), Lane 4: NIH/3T3 (transfected with TrkB; BDNF-treated)

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代表番号: 045-509-1970|電子メール：sales@selleck.co.jp

使用情報

Dilution
WB1:1000 IP1:50

Application
WB, IP

Source
Rabbit Monoclonal Antibody

Reactivity
Human, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
140 kDa

Datasheet & SDS

生物学的記述

Specificity
Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) Antibody [K13K11] detects endogenous levels of total TrkA only when they are phosphorylated at Tyr674/675 and TrkB protein only when they are phosphorylated at Tyr706/707, respectively.

Clone
K13K11

Synonym(s)
High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tyrosine kinase receptor A (Trk-A); gp140trk; p140-TrkA; NTRK1; MTC; NTRK2; TRKB

Background
Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) refers to the autophosphorylated activation loop tyrosines in the kinase domains of TrkA (NTRK1) and TrkB (NTRK2), members of the neurotrophin receptor tyrosine kinase family that also includes TrkC, activated by specific ligands like NGF for TrkA and BDNF/NT4 for TrkB to drive neuronal survival and development. These sites reside within the catalytic kinase domain's activation loop, where dual phosphorylation at Tyr674/675 (TrkA) or Tyr706/707 (TrkB), conserved across family members, stabilizes an active conformation by facilitating substrate access and trans-autophosphorylation, often triggered by ligand-induced dimerization involving transmembrane and juxtamembrane regions. Phosphorylation at these sites marks full kinase activation, enabling downstream signaling through cascades like Ras-MAPK/ERK for proliferation/differentiation, PLCγ for neurite outgrowth, and PI3K/Akt for survival, while mutations or overexpression cause ligand-independent activation promoting oncogenesis in neuroblastomas, breast, and other cancers. In disease contexts, aberrant Phospho-TrkA/B levels correlate with poor prognosis in tumors via autocrine loops and resistance to apoptosis.

Background

Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) refers to the autophosphorylated activation loop tyrosines in the kinase domains of TrkA (NTRK1) and TrkB (NTRK2), members of the neurotrophin receptor tyrosine kinase family that also includes TrkC, activated by specific ligands like NGF for TrkA and BDNF/NT4 for TrkB to drive neuronal survival and development. These sites reside within the catalytic kinase domain's activation loop, where dual phosphorylation at Tyr674/675 (TrkA) or Tyr706/707 (TrkB), conserved across family members, stabilizes an active conformation by facilitating substrate access and trans-autophosphorylation, often triggered by ligand-induced dimerization involving transmembrane and juxtamembrane regions. Phosphorylation at these sites marks full kinase activation, enabling downstream signaling through cascades like Ras-MAPK/ERK for proliferation/differentiation, PLCγ for neurite outgrowth, and PI3K/Akt for survival, while mutations or overexpression cause ligand-independent activation promoting oncogenesis in neuroblastomas, breast, and other cancers. In disease contexts, aberrant Phospho-TrkA/B levels correlate with poor prognosis in tumors via autocrine loops and resistance to apoptosis.

References
[1] Middlemas DS, et al. J Biol Chem. 1994 Feb 18;269(7):5458-66. [2] Cunningham ME, et al. J Biol Chem. 1997 Apr 18;272(16):10957-67.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%